Bacillus acidiceler SYY15 and applications thereof

A bacillus and budding technology, which is applied to Bacillus acid fast-growing bacillus SYY15 and its application fields, to achieve the effect of improving plant disease resistance, obvious effect, and enhancing stress resistance

Active Publication Date: 2020-05-15
GENLIDUO BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps create better plants that are resistant against diseases such as insects or weeds while still producing more nutrients from their roots than traditional methods like fertilizers. It does this through enzymatic processes involving specific types of microbial cells called Acid Fast Growth Bacteria(ASB). These specialized forms have been found effective at decompositing harmful chemical compounds into simpler molecules for use as food sources instead of relying solely upon animal feedings. They help protect soil resources and promote growth without causing environmental pollution.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving crop's ability to resist damage from both physically challenging conditions like high or low temperature during cold weather periods and chemical agents used for fertilizers.

Method used

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  • Bacillus acidiceler SYY15 and applications thereof
  • Bacillus acidiceler SYY15 and applications thereof
  • Bacillus acidiceler SYY15 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and Identification of Bacterial Strain SYY15

[0030] Soil was collected from the experimental demonstration base of Genliduo Biotechnology Co., Ltd. in Weixian County, Hebei Province, and 10 g of soil samples were added to a triangular flask filled with 100 ml of sterile saline. Add 1ml sample to 9ml sterile normal saline, and then dilute 10 2 , 10 3 , 10 4 Take 100 μl of the above soil suspension and spread it evenly on the plate of LB medium (yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, agar 15g / L), and culture it in the incubator at 28°C for 48h. Pick up a single bacterial colony with a sterile toothpick, streak and purify it, and save it for later use.

[0031] Based on the physiological and biochemical characteristics of the strain SYY15 and the results of molecular biology tests, it was identified as Bacillus acidiceler. The specific identification results are as follows:

[0032] 1. Molecular biological identification

[0033] Firstly,...

Embodiment 2

[0040] Example 2 Ability of bacterial strain SYY15 to produce ACC deaminase

[0041] The strain SYY15 was first activated on LB medium. The activated strain was inoculated into DFa medium (KH 2 PO 4 4g, Na 2 HPO 4 6g, MgSO 4 ·7H 2 O 0.2g, glucose 2g, gluconic acid 2g, citric acid 2g, ACC final concentration of 3mM, water 1000ml, pH 7.2), 30°C, 180r / min under shaking table culture for 24h, with the culture solution of no strain As a control, detect the absorbance value of the culture solution at 600nm, and judge whether the strain can grow in the culture solution with ACC as the only nitrogen source according to the absorbance value. If it can grow, it is transferred to DFa culture medium and cultured continuously for 3 times. The results showed that the strain SYY15 could grow in the culture medium with ACC as the sole nitrogen source, OD 600 can reach 2.35 ( figure 1 ).

Embodiment 3

[0042] Example 3 Determination of Phosphorus Solubilizing Ability of Bacterial Strain SYY15

[0043] The strains were first activated on LB medium. Inoculate the activated bacterial strain into the phosphate-dissolving medium shown in Appendix A of NY / T 1847-2010 (glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, distilled water 1000mL, pH 7.0. The organic phosphorus source is calcium phytate, and the addition amount is 2g. Solid medium: add 1.5% agar powder in proportion). Detect whether it has the function of dissolving phosphorus. After testing, the bacterial strain SYY15 has the ability to dissolve organic phosphorus, and the diameter of the dissolution circle for dissolving organic phosphorus reaches 1.34cm ( figure 2 ).

[0044] After confirming that the strain SYY15 has the phosphorus-dissolving function, the phosphorus-dissolving ability of the strain was quantitatively measured. Af...

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Abstract

The invention discloses Bacillus acidiceler SYY15 and applications thereof. The accession number of the strain SYY15 is CCTCC NO: M 2019840; and the strain can synthesize ACC deaminase, can enhance the stress resistance of crops and also can massively degrade insoluble organic and inorganic phosphorus and generate various growth-promoting substances such as IAA and protease. The strain SYY15 can obviously promote the stress resistance effects of plants by taking corns as examples; compared with a salt stress group, the seed germination coefficient of a strain SYY15 treatment group can be increased by 34.10%; and through the application of the strain SYY15 to crop planting, the yield of the crops can be increased, and the stress resistance such as disease resistance and drought resistance of the plants can be enhanced, so that the strain SYY15 has important significance on the sustainable increasing of grain production in China and the protection of farmland ecological environments.

Description

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Claims

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Application Information

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Owner GENLIDUO BIO TECH CO LTD
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