Microbial formulation and method of using the same promote plant growth
A technology to promote plant growth, microbial activator, applied in the direction of fertilizers, applications, fertilizer mixtures made from biological waste
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Embodiment 1
[0054] Example 1: Effect of Selected Trichoderma Strains
[0055] In this example, pot experiments were performed to show that selected Trichoderma microorganisms can increase the efficiency of organic fertilizers. Sandy loam was used as potting substrate, tomato (Lycopersiconesculentum) was used as test plant, and compost was used as organic fertilizer (0.5% w / w). The size of the flower pot is: both diameter and height are 10 cm. Four doses (i.e., 10 1 、10 4 、10 6 and 10 7 CFU / g soil, expressed as doses 1, 2, 3 and 4) Trichoderma microorganisms were added to the potting mix. Soil control samples were uninoculated seedlings where only organic fertilizers were used.
[0056] Measure plant biomass at harvest (38 days) in the form of root dry weight and shoot dry weight, as Figure 1A and Figure 1B shown. according to Figure 1A , the plant biomass in the roots of inoculated seedlings (doses 2, 3 and 4) was significantly greater than the plant biomass in the roots of uni...
Embodiment 2
[0058] Example 2: Effect of microbial blends
[0059] In this example, pot experiments were performed to show that a selected microbial blend comprising Bacillus polymyxa, Bacillus subtilis, Trichoderma harzianum, and Saccharomyces cerevisiae was able to increase the efficiency of organic fertilizers. Sandy loam was used as potting substrate, tomato (Lycopersiconesculentum) was used as test plant, and compost was used as organic fertilizer (0.5% w / w). The size of the flower pot is: both diameter and height are 10 cm. Three doses (i.e., 10 2 、10 3 and 10 4 CFU / g soil, expressed as doses M1, M2 and M3, respectively) of the microbial blends were added to the potting mix. Control samples (Ctrl) were uninoculated seedlings where only organic fertilizers were used.
[0060] Plant height, canopy, stem size and chlorophyll index were measured on day 41 and recorded in Tables 1 to 4 as Figures 2A to 2D shown. according to Figure 2B , the canopy of inoculated seedlings (M1, M2...
Embodiment 3
[0070] Embodiment 3: the effect of microbial activator on Trichoderma bacterial strain
[0071] In this example, Trichoderma microorganisms supplemented with different microbial activators were applied to tomato seedlings in pot experiments to demonstrate the efficacy of the microbial activators. The experimental setup was similar to that described in Example 1. Table 5 summarizes the composition of the various activator formulations administered in this example, which included yeast autolysate, humus powder, and micronutrients with amino acids.
[0072] The composition of the activator preparation of table 5 Trichoderma
[0073]
[0074] Measure plant height, stem size, shoot dry weight, and root dry weight on day 35 as Figures 3A to 3D shown. As shown in the figure, microorganisms supplemented with yeast autolysate, humus powder and / or micronutrients (treatment numbers P0, P2-0.2, P3-8) were compared with plants treated with microorganisms alone (treatment number P0)....
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