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61results about How to "Strong secretory ability" patented technology
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Sludge composting method
ActiveCN101781131AEasy to getShort fermentation timeBio-organic fraction processingClimate change adaptationEcological environmentSludge compost
The invention provides a sludge composting method, and relates to a sludge treatment method which comprises the steps of preferable accessory selection, CK21 fungus spraying inoculation, heavy metal passivation and powder granulation, and then three-dimensional composite biological organic fertilizer with organic, inorganic and microbial active constituents is obtained. Mushroom compost and pig manure are used as accessories which are easily obtained and degraded, the stacker fermentation time is short, only one time of fermentation is needed, the C / N ratio can be effectively adjusted, and the water content of the sludge is reduced; and the efficient beneficial bio-fungus community CK21 is adopted. The microbial concentration is high, the secretion ability of the enzyme is strong, organic matters can be quickly decomposed so as to prevent the corruption of the organic matters, no odor exists, sludge is used for CK21 inoculation, fermentation starts fast, and the effect is good; and the product is inoculated with the CK21 fungus secondarily so as to further balance and activate nutrients, and the microbial natural reproduction is utilized to realize the is called flower dipping absorbing of the plants to nutrients. The invention has the advantages of optimizing the composting conditions, shortening the composting time, reducing energy consumption of turning, and reducing the damage of the heavy metal on the ecological environment.
Owner:厦门市政环境科技股份有限公司
Culture method of mesenchymal stem cells
ActiveCN112522191AImprove proliferative abilityComplete formCulture processSkeletal/connective tissue cellsMesenchymal stem cellSecretion
The invention relates to the technical field of cell culture, and particularly relates to a culture method of mesenchymal stem cells. In the culture method, a serum-free culture medium optimized by the invention is used for culturing the mesenchymal stem cells. In addition, the invention further provides a better culture method for 3D culture. The cells obtained by the culture method disclosed bythe invention are strong in proliferation capacity, intact in shape and strong in secretion capacity, and are in accord with the international quality control standards of the mesenchymal stem cells.Meanwhile, according to the culture method disclosed by the invention, a large number of mesenchymal stem cells (particularly human umbilical cord mesenchymal stem cells) can be obtained by amplifyinga small number of mesenchymal stem cells under the conditions of small space occupation, less culture medium consumption, simpler and more convenient operation and greatly reduced workload; and a solid technical scheme and a theoretical basis are provided for obtaining a large number of high-quality mesenchymal stem cells (especially human umbilical cord mesenchymal stem cells) and secretions thereof in the clinical field.
Owner:YUNNAN KEY LAB OF PRIMATE BIOMEDICINE RES
Serum-free cartilage cell culture solution
ActiveCN104630138AClear outlineGood refractive indexSkeletal/connective tissue cellsGlucanTransferrin
A serum-free cartilage cell culture solution is obtained by adding exogenous additives to a basic culture solution. The basic culture solution is a DMEM culture solution or an F12 culture solution or a DMEM culture solution-F12 culture solution mixed culture solution, and the exogenous additives are nonessential amino acids, glucan, vitamin C, glutamine, sodium pyruvate, insulin or insulin-like growth factor, transferrin, growth hormone, triiodothyronine, hydrocortisone, dexamethasone, sodium selenite, beta-mercaptoethanol, lipid concentrate, progesterone, succinimide, a basic fibroblast growth factor, an epidermal growth factor, a transforming growth factor, a bone morphogenetic protein and a platelet derived growth factor respectively. The serum-free cartilage cell solution can avoid potential risks due to the use of serum, improves the extracellular matrix secreting ability of cartilage cells, makes the growth propagation state approach the growth propagation state of the cells in a serum-containing culture solution, and allows the dedifferentiation rate and the matrix secreting ability of low density inoculation cartilage cells to be obviously better than those of the cells in the serum-containing culture solution respectively.
Owner:SHAANXI RUISHENG BIOTECH
Alginate lyase, host cells capable of secreting alginate lyase and application of alginate lyase and host cells
ActiveCN107904223AExpression realizationImprove securityCosmetic preparationsBacteriaAlginate lyaseHigh activity
The invention provides alginate lyase, host cells capable of secreting alginate lyase and application of the alginate lyase and the host cells. The alginate lyase has any one of amino acid sequences shown as (I) and (II): (I) an amino acid sequence shown as SEQ ID NO. 1; (II) an amino acid sequence obtained by carrying out substitution, deletion or addition of 1 to 20 amino acid residues on the amino acid sequence shown as SEQ ID NO. 1; the obtained amino acid sequence has the activity of the alginate lyase. According to the alginate lyase, whole genes of the alginate lyase are truncated to reduce the molecular weight, so that the expression amount of the host cells on the alginate lyase is remarkably improved; the secreted alginate lyase has high activity, good stability and strong degradation capability on sodium alginate.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Trichoderma reesei strain and application thereof
InactiveCN104263658AStrong secretory abilityMaximum value increasedFungiMicroorganism based processesBiotechnologySecreted substance
The invention discloses a trichoderma reesei strain of which the preservation number is CGMCC No.9644. In addition, the invention also discloses an application of the trichoderma reesei strain in production of cellulose. The trichoderma reesei strain disclosed by the invention has a stronger cellulase production capacity and a stronger protein secretion capacity; compared with original strains, the filter paper activity of the trichoderma reesei strain is increased by 13-31%.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Trichoderma reesei engineering bacterium high in secretion of endoglucanase and beta-glucosidases and construction method and application of trichoderma reesei engineering bacterium
The invention discloses a trichoderma reesei engineering bacterium high in secretion of endoglucanase and beta-glucosidases. The trichoderma reesei engineering bacterium is named as trichoderma reesei(Trichoderma reesei) QMEB5, the genotype of the bacterial strain is QM9414 delta Eegl2 delta Ebgl1, trichoderma reesei QM9414 is used as an original strain, the expression vector pTeg2 of a fusion gene of a gene expression box containing endoglucanase 2 is transformed, then the plasmid pTHB of a gene expression box containing beta-glucosidases is transformed, and screening and verifying are performed, so that the trichoderma reesei engineering bacterium is obtained. The invention further discloses an application of the engineering strain to fermentation production of cellulases. The experiment confirms that the egl2 expression quantity of the engineering strain is 27 times higher than that of the original strain, the bgl1 expression quantity of the engineering strain is 410 times higher than that of the original strain, the activity of the endoglucanase is 117.5IU / mL, and the activity of the beta-glucosidases is 34.5IU / mL. The cellulase system produced through fermentation can be efficiently applied to degradation of corn cobs and other lignocellulolyticenzyme materials, and has favorable industrial development and application prospects.
Owner:SHANDONG UNIV
Method for preparing recombinant duck interleukin-2 protein and its application
InactiveCN1544630AImprove stabilityHigh purityAntibody ingredientsDepsipeptidesDiseaseEscherichia coli
The invention discloses a recombinant duck interleukin-2 protein preparing method and its use, the DNA fraction of RT-PCR amplified duck interleukin-2c of different ducks and prokaryotic expression carrier pBAD / HisB, as well as eukaryotic expression carrier pMET alpha A to compose high-efficacy expression particles, recombining and converting these carriers to Escherichia coli LMG194, and microzyme strains PMAD11 and PMAD16, respectively and then inducing expression. After inducing, making ultrasonic wave cracking and deposit elimination by centrifugation on Escherichia coli to obtain crude products of duck interleukin-2 and using protein purifying system to obtain duck interleukin-2 pure products. The pure or crude of duck interleukin-2 can act as immune assistant, anti-disease additive and disease-curing drug. The duck interleukin-2 monoclonal and polyclonal antibodies have simple preparing procedure, and can act as good immune inhibitor. It applied genetic engineering technique to prepare recombinant protein gsIL-2, and has simple technical flow, low production cost, good stability, high bioactivity and other characters.
Owner:ZHEJIANG UNIV
Thelephora ganbajun Zang exopolysaccharide, preparation method thereof and application of exopolysaccharide
ActiveCN106636252AStrong secretory abilityImproves antioxidant activityCosmetic preparationsOrganic active ingredientsThelephora ganbajunFermentation broth
The invention relates to the technical field of thelephora ganbajun Zang polysaccharides, in particular to a thelephora ganbajun Zang exopolysaccharide. A preparation method of the thelephora ganbajun Zang exopolysaccharide includes the steps: fermenting thelephora ganbajun Zang strains TG-1; extracting the thelephora ganbajun Zang exopolysaccharide from fermentation broth; separating mixture by the aid of an anion exchange column chromatography; eluting by the aid of deionized water, 0.1 mol / L of NaCl solution and 0.3 mol / L of NaCl solution to respectively obtain thelephora ganbajun Zang exopolysaccharide components such as EPS-1, EPS-2 and EPS-3. The preservation number of the thelephora ganbajun Zang strains TG-1 is CGMCC No.12977. The thelephora ganbajun Zang EPS has good humidity preservation and antioxidant activity, antioxidant ability of the EPS-2 in the three components is highest, and the EPS is high in the humidity preservation ability. The thelephora ganbajun Zang exopolysaccharide can be widely applied to anti-aging health care products and cosmetics.
Owner:BIOLOGY INST OF SHANDONG ACAD OF SCI
Preparation method and application of oxalate decarboxylase
ActiveCN106939315AStrong secretory abilityHigh densityVectorsFermentationBiotechnologySequence signal
The invention discloses a preparation method of oxalate decarboxylase. The method comprises the following steps: constructing a recombinant expression plasmid vector comprising Bacillus-containing acid induced promoter, a secretion signal peptide, and a fungus-derived oxalate decarboxylase gene prepared under the control of the secretion signal peptide, transforming bacilli normally growing in a pH value being not more than 4.5 by the vector to prepare oxalate decarboxylase recombinant bacteria; and carrying out culture and acid feeding on the recombinant bacteria in a fermentation medium, inducing the recombinant bacteria, and carrying out simple purification to recover generated extracellular oxalate decarboxylase. The extracellular oxalate decarboxylase prepared through the method is used in diagnosis reagent raw materials, medicinal compositions, healthcare foods or formulated foods with special medical uses. The mutated acid induced promoter is adopted to substitute an original acid induced promoter in the recombinant expression vector, so the transformed bacilli have a high expression level.
Owner:WUHAN KANGFUDE BIOTECH CO LTD
Concentrated adipose-derived stem cell culture supernatant preparation and preparation method and application thereof
InactiveCN107714727APromote repairLittle side effectsPeptide/protein ingredientsUnknown materialsPositive controlCuticle
The invention belongs to the technical field of medical treatment and health and particularly relates to a concentrated adipose-derived stem cell culture supernatant preparation to treat skin scalding. The concentrated adipose-derived stem cell culture supernatant preparation is prepared with supernatant of a cell culture fluid cultured via adipose-derived mesenchymal stem cells; cell factors hrEGF, VEGF and bFGF are added to the preparation. The invention also provides a preparation method and application of the concentrated adipose-derived stem cell culture supernatant preparation. The concentrated adipose-derived stem cell culture supernatant preparation of the invention can significantly promote healing of scald wounds, and the results lie in the aspect: after the use of the preparation, epidermis and dermis can heal and restore their functionality faster than those treated in a positive control and other embodiment groups. In addition, the concentrated adipose-derived stem cell culture supernatant preparation of the invention is free of living cell ingredients, no ethic issues or immune safety issues occur, compounding the preparation when it is needed is not required, and thepreparation is convenient to apply.
Owner:成都远山博桥生物科技有限公司
Method for inducing human umbilical cord mesenchymal stem cells into testicular interstitial cells and application thereof
InactiveCN102533659AImprove proliferation efficiencyLow immunogenicitySkeletal/connective tissue cellsViruses/bacteriophagesTesticular Interstitial CellsMesenchymal stem cell
The invention discloses a method for inducing human umbilical cord mesenchymal stem cells into testicular interstitial cells and application thereof. The method comprises the following steps: cloning mouse steroidogenic factor-1 (SF-1) gene into an adenovirus shuttle plasmid pAdtrack-CMV-EGFP to construct a recombinant vector pAdtrack-CMV-SF-1; recombining the recombinant vector pAdtrack-CMV-SF-1 and an adenovirus carrier plasmid pAdEasy-1 to obtain an adenovirus plasmid pAd-SF-1 carrying SF-1 gene; digesting and linearizing the adenovirus plasmid pAd-SF-1, and packaging with human embryonic kidney cells AD-293 to obtain adenovirus; adding the adenovirus into an induction culture medium to infect human umbilical cord mesenchymal stem cells, and carrying induced differentiation of the human umbilical cord mesenchymal stem cells to testicular interstitial cells. Through the induction using the method of the invention, human umbilical cord mesenchymal stem cells can in vitro differentiate into testicular interstitial cells only in one week, so as to provide an important cell source for treatment of testosterone deficiency using cell replacement or genetic method.
Owner:JINAN UNIVERSITY
Transglutaminase mutant expressed in active form
ActiveCN107746836AAchieve active expressionEasy to separate and purifyFungiMicroorganism based processesBiotechnologyAmine transaminase activity
The invention discloses a transglutaminase mutant expressed in an active form, belonging to the fields of enzyme engineering and fermentation engineering. According to the technical scheme disclosed by the invention, site-directed mutation is carried out on a mature region of transglutaminase, so that amino acid residues near the active site of the transglutaminase are changed, the catalytic efficiency of transglutaminase is improved, and recombinant bacterium hpro-E300W with the enzyme activity of the transglutaminase further improved is obtained. The fermentation enzyme activity of a recombinant bacterium hpro-E300W fermentation tank can reach 59.85U / mL, which is the maximal value of the currently reported fermentation level. In addition, by inserting yarrowia lipolytica endogenous proteinase Kex2 recognition sites in transglutaminase, the active expression of the transglutaminase in the yarrowia lipolytica is embodied. During the fermentation production, little impure protein is generated, the purification is simple, and the cost of industrial production of transglutaminase can be reduced.
Owner:JIANGNAN UNIV
Preparation method of DCs, and application of DCs in preparing anti-tumor cell preparation
InactiveCN103602634AExcellent adhesionIncrease the number ofMammal material medical ingredientsBlood/immune system cellsDendritic cellPolyvinyl chloride
The invention discloses a preparation kit and a preparation method of adherent mononuclear cells, immature DCs (Dendritic Cells) and mature DCs, and an application of the mature DCs prepared by the method in preparing an anti-tumor cell preparation, and belongs to the biological technical field. According to the preparation kit, the preparation method and the application of the mature DCs, which are disclosed by the invention, the adherent mononuclear cells are prepared from CD14 monoclonal antibodies through a culture bottle dish made of a polystyrene, polyvinyl chloride or polyethylene material; the immature DCs are prepared from GM-CSF (granulocyte-macrophage colony-stimulating factor), IL-4 (interleukin-4) and IFN (interferon)-alpha; the mature DCs are prepared from TNF (tumor necrosis factor)-alpha and OK-432. The method disclosed by the invention is capable of increasing the adherence ability of the adherent mononuclear cells and increasing the number of the adherent cells, and thus being advantageous for reducing the influence of impurity cells on the transformation of the adherent mononuclear cells into the immature DCs; besides, the method is capable of increasing the yield of the immature DCs; the method is capable of obtaining mature DCs which are higher in maturity, and better in cell migration ability and IL-12p70 secretion ability.
Owner:山东迪博生物科技股份有限公司
Mesenchymal stem cell composite active factor freeze-dried powder as well as preparation method and application thereof
ActiveCN110974944ASufficient sourceEasy accessPowder deliveryOrganic active ingredientsCulture mediumsCutis
The invention relates to mesenchymal stem cell composite active factor freeze-dried powder as well as a preparation method and application thereof. In the preparation process of the composite active factor freeze-dried powder, a specific pre-coating solution is used for treating a cell culture container; and mesenchymal stem cells are cultured by adopting a first culture medium and a second culture medium under specific culture conditions, so the mesenchymal stem cells can be still kept at a normal state in the processes of hunger culture and induction and secrete more active factors. The composite active factor freeze-dried powder prepared by the invention can promote cell division and cell proliferation, repair damaged skin or skin with thin congenital cuticle, tighten the skin and reduce wrinkles; moreover, the immunoregulation function of the skin is activated, the metabolism of the skin is promoted, and an anti-aging effect is achieved.
Owner:广州赛加生物科技有限公司
Neutral proteinase gene constructed by molecular chaperone prsA, protein, bacillus subtilis, preparation and application
InactiveCN108070606AImprove expression levelStrong secretory abilityBacteriaHydrolasesNeutral proteinaseMicrobiology
The invention belongs to preparation of a new gene and construction of an engineering bacterium, and particularly relates to a neutral proteinase gene constructed by a molecular chaperone prsA, a protein, bacillus subtilis, preparation and an application. The molecular chaperone PrsA and the neutral proteinase gene are integrated to construct pHT43-npr-PrsA recombinant plasmid by a molecular biology technology and finally converted into a host bacterium, namely the bacillus subtilis WB800N, to construct a new bacillus subtilis engineering bacterium CGMCC No. (China General Microbiological Culture Collection Center number) 14837; the molecular chaperone PrsA and the neutral proteinase gene are co-expressed; and an expression level of neutral proteinase is effectively increased.
Owner:河北省微生物研究所有限公司 +1
Neutral protease gene built by molecular chaperone DnaK, protein, bacillus subtilis, and preparation and application of neutral protease gene built by molecular chaperone
InactiveCN107881181AImprove expression levelStrong secretory abilityBacteriaHydrolasesNeutral proteaseBacillus subtilis
The invention belongs to new gene preparation and engineering bacterium building, and particularly relates to a neutral protease gene built by a molecular chaperone DnaK, protein, bacillus subtilis, and preparation and application of the neutral protease gene built by the molecular chaperone DnaK. The molecular biology technology is used for integrating the molecular chaperone DnaK with the neutral protease gene; the pHT43-npr-DnaK recombinant plasmids are built and are finally converted into a host bacterium of bacillus subtilis WB800N; a novel bacillus subtilis engineering strain CGMCC (China General Microbiological Culture Collection Center) No.14836 is built, so that the molecular chaperone DnaK and the neutral protease gene realize the coexpression; the expression level of the neutralprotease is effectively improved.
Owner:河北省微生物研究所有限公司 +1
Method for preparing recombinant duck interleukin-2 protein and its application
InactiveCN1231585CImprove stabilityHigh purityAntibody ingredientsDepsipeptidesDiseaseEscherichia coli
Owner:ZHEJIANG UNIV
Transformation method for bacillus amyloliquefaciens
InactiveCN110423784ASolve hard-to-transform problemsEfficiently obtainedOther foreign material introduction processesGlycineCompetent cell
The invention relates to a transformation method for bacillus amyloliquefaciens, and belongs to the technical field of gene engineering. In order to solve the problem of difficult transformation of the bacillus amyloliquefaciens, the invention provides a transformation method for the bacillus amyloliquefaciens. The transformation method includes the following steps that the bacillus amyloliquefaciens are cultured by an LB+0.5 M sorbitol liquid medium, glycine is added to make the final concentration reach 7-8 mg / mL when culturing is carried out for 3-5 h, the bacillus amyloliquefaciens are continuously cultured to OD 600 to be 0.9, thallus is collected centrifugally, the thallus is placed into an electrotransfer medium to obtain efficient competent cells after rinsing, plasmids are conducted into the competent cells through electrotransformation, and after the electrotransformation, positive transformants can be obtained rapidly and efficiently by screening LB plates containing corresponding antibiotics. The invention provides a method for transformation in the bacillus amyloliquefaciens. According to the method, more positive transformants can be obtained rapidly, and the positive rate can reach about 80%.
Owner:INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
Mesenchymal stem cell serum-free medium and application thereof
ActiveCN112608891AClear chemical compositionAvoid instabilityCulture processSkeletal/connective tissue cellsSerum freeMesenchymal stem cell
The invention relates to the technical field of cell culture, and particularly relates to a mesenchymal stem cell serum-free medium and application thereof. The mesenchymal stem cell serum-free medium comprises a basic medium and additive components, wherein the basic medium is an IMDM medium; the additive components comprise TGF-[beta]3 and ascorbic acid, the concentration of TGF-[beta]3 is 1-5 [mu]g / L, and the dosage ratio of ascorbic acid to TGF-[beta]3 is 25-200mg: 1-5[mu]g. The medium disclosed by the invention has a good cell adherence promoting effect, and the cultured cells are strong in multiplication capacity, complete in form and strong in secretion capacity. Compared with a traditional serum culture system and an existing serum-free system, the serum-free medium is clear in chemical components, free of human-derived or animal-derived protein and free of serum, and the instability of animal-derived components and batches is avoided.
Owner:YUNNAN KEY LAB OF PRIMATE BIOMEDICINE RES
Recombinant bacillus subtilis of exocytosis PNAG polysaccharide and application of recombinant bacillus subtilis
ActiveCN110184231AExtensive culture conditionsClear genetic backgroundBacteriaMicroorganism based processesMetabolic engineeringProbiotic
The invention discloses recombinant bacillus subtilis of exocytosis PNAG polysaccharide. A PNAG polysaccharide synthetase coding gene and expression plasmid are connected, recombinant expression plasmid is constructed, then the recombinant expression plasmid is transferred into bacillus subtilis, and the recombinant bacillus subtilis is obtained. The invention further discloses a construction method of the recombinant bacillus subtilis. A new metabolism synthetase gene is guided into bacillus subtilis, the recombinant bacillus subtilis is successfully constructed, PNAG polysaccharide can be produced through fermentation, the produced exocytosis PNAG polysaccharide can reach 136mg / L, and a base is established for further reforming the bacillus subtilis for producing the PNAG polysaccharidethrough metabolic engineering. The invention further discloses an application of the recombinant bacillus subtilis to preparation of products containing the PNAG polysaccharide through fermentation indifferent fields of foods, medicines and chemical industry. The PNAG polysaccharide produced from recombinant strains can promote growth of probiotics and restrain harmful bacteria, and has good application prospects in many fields.
Owner:杭州丰海生物科技有限公司
Recombinant pichia pastoris strain for regulating and controlling transglutaminase expression of zea mays through TEF1 promoter and construction method
InactiveCN109749948AEasy to trainStrong secretory abilityFungiTransferasesPichia pastorisBiotechnology
The invention relates to a recombinant pichia pastoris strain for regulating and controlling transglutaminase expression of zea mays through a TEF1 promoter. The recombinant pichia pastoris strain isa genetically engineered strain obtained in the mode that transglutaminase from the zea mays is transferred to pichia pastoris. According to the recombinant pichia pastoris strain, an alcohol oxidasepromoter PAOX1 in a pPIC9K plasmid is replaced by a promoter PTEF1 of a translation extension factor 1-alpha, the promoter PTEF1 is connected with a transglutaminase gene to form a loop, and a recombinant expression vector is constructed. The recombinant strain is easy to culture, high in secretion capacity and beneficial for mass transglutaminase expression, the enzyme activity can reach 479 mU / mL, and the recombinant pichia pastoris strain has the advantages of being safe and reliable and has important application prospects.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
A transglutaminase mutant with improved enzyme activity
ActiveCN107739734BEasy to separate and purifyEasy to trainFungiAcyltransferasesGlutamine aminotransferaseSite-directed mutagenesis
The invention discloses a glutamine transaminase mutant with improved enzymatic activity, which belongs to the field of enzyme engineering. The site-specific mutagenesis is performed on glutamine transaminase, an amino acid residue close to an active site of the glutamine transaminase is changed, so that the catalytic efficiency of the glutamine transaminase is improved, and the enzymatic activityof the glutamine transaminase is further improved. Recombinant yarrowia lipolytica with improved enzymatic activity is established by the glutamine transaminase mutant, the enzymatic activity of theglutamine transaminase is improved by 1.45 times compared with an original strain, the enzymatic activity of the shake flask fermentation can reach 16.995U / mL, and the enzymatic activity of the fermenting tank fermentation can reach 59.85U / mL which is the highest fermentation value reported at present.
Owner:TAIXING DONGSHENG FOOD TECH
Antibodies to tigit and uses thereof
ActiveCN114106182AReduce the effect of suppressing immune cellsPromote T cell activityHybrid immunoglobulinsImmunoglobulins against cell receptors/antigens/surface-determinantsAntigenAntiendomysial antibodies
The invention belongs to the field of biological medicine, and relates to an anti-TIGIT antibody and application thereof. Specifically, the invention relates to an anti-TIGIT antibody or an antigen binding fragment thereof, the antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequences of HCDR1 to HCDR3 of the heavy chain variable region are respectively shown as SEQ ID NOs: 3-5, and the amino acid sequences of LCDR1 to LCDR3 of the light chain variable region are respectively shown as SEQ ID NOs: 8-10; and according to an EU numbering system, the heavy chain constant region comprises mutation such as L234A and L235A. The antibody disclosed by the invention can be effectively combined with TIGIT, has small toxic and side effects and has the potential of being applied to tumor prevention and treatment.
Owner:AKESO BIOPHARMA
Aspergillus sydowii and application thereof in promoting plant growth and preventing and treating plant diseases
ActiveCN112680360AHas nitrogen fixationStrong secretory abilityBiocidePlant growth regulatorsBiotechnologyPlanting seed
The invention relates to the technical field of microorganisms and biology, in particular to aspergillus sydowii as well as a separation method and application thereof. The aspergillus sydowii JDQMZZ-1 has been deposited in China General Microbiological Culture Collection Center on March 12, 2020 with a deposit number of CGMCC No.19278, and has nitrogen fixation and strong secretion ability; has a strong inhibitory effect on fungi and bacteria, can also promote germination of plant seeds, and can achieve growth-promoting effects by adjusting a content of plant endogenous hormones; can promote growth of underground part of seedlings of Paris polyphylla after applying to the seedlings of Paris polyphylla, but does not activate plant autoimmunity, and effectively avoids a result of activating plant autoimmunity leading to reduced biomass. Meanwhile, the aspergillus sydowii JDQMZ-1 increases chlorophyll content of plants and reduces malondialdehyde content, which indicates that when the aspergillus sydowii JDQMZ1 acts on seedlings of Paris polyphylla, an influence of the environment on the seedlings of Paris polyphylla is reduced, damage to cell membranes is reduced, and abiotic stress resistance of the seedlings of Paris polyphylla is improved.
Owner:QINGHAI NORMAL UNIV +1
Method for preparing stomach-regulating pills
ActiveCN101947298AImprove stabilityControllable extractionDigestive systemAntiinfectivesStomachDrugs stability
The invention discloses a method for preparing stomach-regulating pills, which comprises the following steps of: respectively performing volatile oil extraction, water temperature extraction and alcohol extraction on different medicinal materials in the stomach-regulating formula, extracting effective parts therein, and mixing to obtain an intermediate for preparing the pills; and mixing the intermediate and a substrate, dispersing into the substrate, and performing a pill forming process to obtain the stomach-regulating pills. In the pill preparing process, each extraction link and the forming process have controllable quality, the content of active ingredients is stable, the pill preparation is carried out after the medicament intermediate is mixed fully with the substrate, and the medicament active ingredients are highly dispersed, so the prepared pills have high medicament stability.
Owner:陕西中药研究所
Novel method for cultivating large free pearls by pinctada maxima
ActiveCN113207769AHigh retention rateImprove survival rateClimate change adaptationPisciculture and aquariaPinctada margaritiferaHorticulture
The invention relates to the technical field of pearl cultivation, in particular to a novel method for cultivating large free pearls by pinctada maxima. The novel method mainly comprises the following steps of: cutting off 30-40% of retractor of pinctada maxima pearl cultivating shells, reducing the extrusion strength of the retractor on pearl nucleuses, implanting only one perfect circular pearl nucleus at a left bag nucleus position for the first nucleus implantation, and during the second and third nucleus implantation, implanting one pearl nucleus at the left bag nucleus position and a right bag nucleus position respectively. The pinctada maxima belongs to rare protected species, in order to make full use of the pearl cultivation potential of the pinctada maxima, nucleus implantation is carried out three times in total, and meanwhile, the diameter of harvested pearls is 2.0-3.0 mm larger than that of pearls obtained in the previous step every time nucleus implantation is added; and by adopting the nucleus implantation cultivation method, the pearl cultivation capacity of the pinctada maxima is utilized to the maximum extent, the survival rate, the nucleus remaining rate and the superior pearl rate of the pinctada maxima are increased, and large free pearl industrial cultivation of the pinctada maxima can be achieved.
Owner:BEIBU GULF UNIV
SD rat-derived mesenchymal stem cell induction medium and induction method
ActiveCN112708595ASimple ingredientsSimple procedureCulture processSkeletal/connective tissue cellsBone marrow mesenchymal stem cellsRat Bone Marrow
The invention discloses an SD rat-derived mesenchymal stem cell induction medium and induction method. The induction medium is composed of an alpha-MEM medium and an additive, wherein the additive is composed of 5-100 ng / ml of a transforming growth factor-alpha, 10-200 ng / ml of regulatory protein [beta]1, 100-3000 nM of trehalose and 50-5000 nM of dibutyryl cyclic adenosine monophosphate according to the final concentration. According to the SD rat-derived mesenchymal stem cell induction culture medium and induction method, the secretion amount of factors such as BDNF, HGF, VEGF and GDNF of rat mesenchymal stem cells can be obviously increased, and the secreted factors are relatively rich in variety and can be stably secreted for a long time; and the risks and uncertainty caused by other methods such as gene modification are avoided through a cytokine induction method, and the induction method is more suitable for industrialization and clinical application.
Owner:北京益华生物科技有限公司
A kind of preparation method and application of oxalate decarboxylase
The invention discloses a preparation method of oxalate decarboxylase. The method comprises the following steps: constructing a recombinant expression plasmid vector comprising Bacillus-containing acid induced promoter, a secretion signal peptide, and a fungus-derived oxalate decarboxylase gene prepared under the control of the secretion signal peptide, transforming bacilli normally growing in a pH value being not more than 4.5 by the vector to prepare oxalate decarboxylase recombinant bacteria; and carrying out culture and acid feeding on the recombinant bacteria in a fermentation medium, inducing the recombinant bacteria, and carrying out simple purification to recover generated extracellular oxalate decarboxylase. The extracellular oxalate decarboxylase prepared through the method is used in diagnosis reagent raw materials, medicinal compositions, healthcare foods or formulated foods with special medical uses. The mutated acid induced promoter is adopted to substitute an original acid induced promoter in the recombinant expression vector, so the transformed bacilli have a high expression level.
Owner:WUHAN KANGFUDE BIOTECH CO LTD
A kind of grouper feed additive and using method thereof
ActiveCN104186960BPromote growthImprove non-specific immune functionAnimal feeding stuffBiotechnologyLactobacillus
The invention discloses a grouper feed additive and a method for using the same. It is composed of photosynthetic bacteria liquid, Bacillus pumilus powder and Lactobacillus acidophilus liquid. First, the Bacillus pumilus powder is mixed with the Lactobacillus acidophilus liquid and then mix the obtained mixed bacteria with the photosynthetic bacteria liquid; the mass percent content ranges of the three components are: the photosynthetic bacteria liquid is 18% to 22%, the Bacillus pumilus powder is 1% to 3%, and the acidophilic The lactobacillus liquid is 75-81%; add 10g-50g of additives to 1kg of basic feed, mix well with the basic feed and wrap it with emulsified fish oil. The invention is applicable to the healthy cultivation of the grouper, can obviously improve the activity of SOD, AKP and lysozyme in the fish body, thereby effectively promoting the growth of the grouper.
Owner:GUANGZHOU UNIVERSITY
High-yield recombinant strain of trichoderma harzianum alpha-1, 3-glucanase and application thereof
ActiveCN108795781AStrong secretory abilityIncrease positive rateFungiMicroorganism based processesBiotechnologyProtein target
The invention discloses a high-yield recombinant strain of trichoderma harzianum alpha-1, 3-glucanase and an application thereof. The high-yield recombinant strain of trichoderma harzianum alpha-1, 3-glucanase and the application thereof are characterized in that alpha-1, 3-glucanase genes from trichoderma harzianum CCM F-340 conduct codon optimization; with pyr4 genes used as screening marker genes, trichoderma reesei cbh1 strong promoter and signal peptide sequence thereof and cbh1 terminator are used to construct alpha-1, 3-glucanase expression vector pSK-mutAW; highly secreted expression of the trichoderma harzianum alpha-1, 3-glucanase in trichoderma reesei is achieved. Experiments show that the high-yield recombinant strain of trichoderma harzianum alpha-1, 3-glucanase and the application thereof have the advantages that recombinant expression of the trichoderma harzianum alpha-1, 3-glucanase can effectively degrade non-water-soluble glucan mutan with fermentation enzyme activityunit reaching 2.3 milliliters and alpha-1, 3-glucanase content in fermentation broth being about 68 micrograms per milliliter; fermentation process and target protein purification process of the sameare simple and very suitable for industrial production and application.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
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