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Thelephora ganbajun Zang exopolysaccharide, preparation method thereof and application of exopolysaccharide

A technology of extracellular polysaccharide and dry bacteria cell, which is applied in the field of dry bacteria polysaccharide and can solve the problems of insufficient utilization and the like

Active Publication Date: 2017-05-10
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of insufficient utilization of exopolysaccharide EPS in the above prior art in the extraction and application of D. Thelephoraganbajun Zang) Mycelium exopolysaccharide EPS obtained by fermentation of TG-1

Method used

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  • Thelephora ganbajun Zang exopolysaccharide, preparation method thereof and application of exopolysaccharide
  • Thelephora ganbajun Zang exopolysaccharide, preparation method thereof and application of exopolysaccharide

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Experimental program
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Embodiment 1

[0039] The acquisition of the strain of embodiment 1

[0040] 4 fresh dry fungus fruiting bodies were collected from different areas in Yunnan Province, and the surface was disinfected with 75% alcohol, and then rinsed with sterile water for several times. On the potato dextrose (PDA) medium plate, it was transferred to a 24 °C incubator for cultivation. After the hyphae grew, the purification was repeated until the pure culture of dry bacteria was obtained. Four dry bacteria strains were screened from the four dry bacteria fruiting bodies, and named TG-1, TG-2, TG-3, TG-4 respectively.

Embodiment 2

[0041] Example 2 Extraction of dry bacteria exopolysaccharide (EPS)

[0042]Carry out fermenter culture experiments, ① activation of strains. The dry bacteria TG-1, TG-2, TG-3 and TG-4 were inoculated on the activated medium plates (potato 200 g / L, glucose 20 g / L, magnesium sulfate 1 g / L, potassium dihydrogen phosphate 1.5 g / L) and cultured at 24 °C for 7 d. Take 0.5 cm 2 Dried bacteria activated strains were added to liquid medium (potato 200 g / L, glucose 20 g / L, peptone 2 g / L, magnesium sulfate 1 g / L, potassium dihydrogen phosphate 1.5 g / L), 500 mL triangular bottle The liquid volume was 300 mL, and then cultured with shaking at 24 °C for 10 d. ② Culture conditions. Loading volume: 7.5 liters; inoculation volume: 10%; fermentation temperature: 24°C; stirring speed: 350 r / min; foam control: add two drops of defoamer after inoculation. ③ Determination of pH value and DO value during fermentation. Use pH electrode and dissolved oxygen electrode to automatically measure an...

Embodiment 3

[0044] Example 3 In vitro antioxidant experiments

[0045] (1) Determination of reducing power

[0046] The reducing power was measured using the Prussian blue method. The exopolysaccharides of dry bacteria were diluted with deionized water in a gradient manner, and a series of polysaccharide aqueous solutions of different concentrations were prepared. Take 1 mL of polysaccharide sample into a test tube, add 2.5 mL of phosphate buffer (pH 6.6, 0.2 mol / L) and 1 mL of potassium ferricyanide solution (10 g / L), mix well and place it in a constant temperature water bath at 50 °C 20min. After the water bath, 2 mL of trichloroacetic acid (100 g / L) and 1.2 mL of ferric chloride solution (1 g / L) were added. After mixing, the absorbance at 700 nm was measured, and 2,6-di-tert-butyl-4-methylphenol (BHT) was used as a positive control.

[0047] (2) Determination of hydroxyl radical scavenging ability

[0048] The hydroxyl radical scavenging ability was determined by the Fenton method...

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Abstract

The invention relates to the technical field of thelephora ganbajun Zang polysaccharides, in particular to a thelephora ganbajun Zang exopolysaccharide. A preparation method of the thelephora ganbajun Zang exopolysaccharide includes the steps: fermenting thelephora ganbajun Zang strains TG-1; extracting the thelephora ganbajun Zang exopolysaccharide from fermentation broth; separating mixture by the aid of an anion exchange column chromatography; eluting by the aid of deionized water, 0.1 mol / L of NaCl solution and 0.3 mol / L of NaCl solution to respectively obtain thelephora ganbajun Zang exopolysaccharide components such as EPS-1, EPS-2 and EPS-3. The preservation number of the thelephora ganbajun Zang strains TG-1 is CGMCC No.12977. The thelephora ganbajun Zang EPS has good humidity preservation and antioxidant activity, antioxidant ability of the EPS-2 in the three components is highest, and the EPS is high in the humidity preservation ability. The thelephora ganbajun Zang exopolysaccharide can be widely applied to anti-aging health care products and cosmetics.

Description

technical field [0001] The present invention relates to the technical field of dry pasteurium polysaccharide, in particular to a dry pasteurium exopolysaccharide, and also relates to a preparation method and application of the dry pasteurium exopolysaccharide. Background technique [0002] dry mushroom ( Thelephora ganbajun Zang) is a rare wild edible fungus of the genus Thelephora unique to my country, with good taste and high nutritional value. The dry mushroom has an ectomycorrhizal relationship with the pine tree, and artificial cultivation is difficult to succeed. Therefore, the disorderly harvesting of the dry mushroom is serious, and the output decreases year by year. Due to the limitation of its distribution, the researches on the dry bacteria at home and abroad are extremely limited, mainly focusing on the classification of dry bacteria, bacterial species isolation, bacterial species identification and ecological environment. Therefore, dry bean is a strain r...

Claims

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Application Information

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IPC IPC(8): C12P19/04A23L33/125A61K31/715A61P39/06A61K8/9728A61K8/73A61Q19/00A61Q19/08C12R1/645
Inventor 孟庆军郑岚杨艳蔡雷马耀宏刘庆艾
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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