38 results about "Pinctada margaritifera" patented technology

The invention relates to a breeding method for a rapid growing line of seawater pearl shells, which comprises the steps of: taking a certain population of Pinctada martensii Dunker as a basic population, firstly selecting individuals with shell width being 10-40 percent higher than average value from the basis population by taking shell width as a selection index, then selecting individuals with shell height or weight being 10-60 percent higher than average value as breeding parents, dissecting the breeding parents, taking sexual glands to conduct artificial insemination, selecting and reserving preparative parents at a young shell stage according to a proportion of 20-30 percent, and finally conducting more than three generations of continuous selection to obtain the rapid-growing large excellent Pinctada martensii Dunker line (variety). The breeding method has the advantages that the method is simple, convenient and feasible, the cost for nursing the parents can be reduced, the growth speed and the individual size of the Pinctada martensii Dunker can be effectively improved and the pearl producing effect is improved.

The invention relates to a method for processing a mantle piece and for improving the quality of sea-pearl. The method is characterized as follows: chitobiose after isolation and purification is selected and is prepared into solution with the density of 0.5 to 3 percent by degerming seawater; the outer margin of non-pigment particles of the mantle which has strong fissionability is cut from the mantle piece shell, after the mantle piece is soaked for 5 to 10 minutes or placed for about 10 minutes after being applied with solution with similar density, the mantle piece is cut into a mantle piece with the length and width of both 4.5 to 5.5mm; the mantle piece which is processed by the chitobiose solution and a pearl core are put into a pearl culture bag that cultures pinctada margaritifera, and after 15 days, the pearl culturing shell which is implanted with the pearl core and the mantle piece which is processed by the chitobiose solution is transferred to mariculture water to culture pearl. The method can promote the division of the cell of the mantle piece effectively, can fasten the formation of the pearl sac and inhibit bacterial infection of operation, and the survival rate of the pear culturing shell, the core reserving rate and the excellent pearl rate can be increased by 20 percent, 15 percent and 15 percent respectively. The method has the advantages of simple implementation and obvious effect.

The invention provides methods and compositions useful in pearl oyster cultivation. Polynucleotide and polypeptides relating to the nacre gene of Pinctada margaritifera are provided. Antibodies related to these polypeptides, and compositions comprising polynucleotides, polypeptides and / or antibodies of the invention are also provided. The invention provides methods of using these polynucleotides, polypeptides and antibodies, including methods related to pearl oyster cultivation. Arrays comprising polynucleotides, polypeptides and / or antibodies of the invention are also provided.

The invention discloses a method for cultivating pinctada martensi. In the method, the pinctada martensi and chlamys nobilis are cultivated in proportion in the cultivation process. By utilizing the method for cultivating the pinctada martensi disclosed by the invention, the growth rate and survival rate of the pinctada martensi can be obviously improved, biomass liveweight adhered to the pinctada martensi is lowered, the cultivation time of the pinctada martensi is shortened and the economic benefits of products can be enhanced; and the method is suitable for popularization and application in aquaculture.

The invention provides methods and compositions useful in pearl oyster cultivation. Polynucleotide and polypeptides relating to the nacre gene of Pinctada margaritifera are provided. Antibodies related to these polypeptides, and compositions comprising polynucleotides, polypeptides and / or antibodies of the invention are also provided. The invention provides methods of using these polynucleotides, polypeptides and antibodies, including methods related to pearl oyster cultivation. Arrays comprising polynucleotides, polypeptides and / or antibodies of the invention are also provided.

The invention relates to aquatic feeds, and particularly discloses pinctada martensii micro-encapsulated diet and a preparation method thereof. The pinctada martensii micro-encapsulated diet is prepared from core raw materials and wall raw materials, wherein the core raw materials comprise the following components in percentage by weight: 10-15 percent of fish powder, 5-15 percent of enzyme hydrolyzed soybean meal, 40-60 percent of kelp powder, 5-10 percent of yeast autolytic powder, 5-15 percent of spirulina powder, 10-20 percent of alpha-starch, 1-2 percent of compound minerals, 0.3-0.6 percent of vitamin complex and 0.1-0.3 percent of choline. According to the preparation method, the core raw materials and the wall raw materials are grinded and mixed to prepare the micro-encapsulated diet by utilizing a spray-drying method. The micro-encapsulated diet is fully-artificial feed for feeding habits and nutritional requirement of pinctada martensii, and is used for providing raw material guarantee for establishing large-scale industrial aquaculture and pearl production mode of pinctada martensii.

The invention relates to the technical field of marine product shellfish breeding, in particular to a method for cultivating a new variety of pinctada fucata small shellfish. The method specifically comprises the following steps: (1) establishing a basic group, (2) selecting parent shellfish, (3) obtaining gametes, (4) constructing a first-generation breeding group F1, and (5) constructing and breeding generations F2 to F4 or above. According to the method, through specialized breeding, a new pinctada fucata small shellfish variety with high nacre secretion capacity and high nacre glossiness is obtained, and the defects in the prior art are overcome.

The invention discloses an artificial seedling raising method of Pinctada margaritifera, which comprises the steps of treating water for seedling raising, selecting parent Pinctada margaritifera, obtaining fertilized eggs, breeding larvae, putting seedling collectors and the like. Relatively stable environmental conditions are provided for the growth and development of the larvae and the seedlings by controlling the illumination intensity, the water changing frequency and the feeding amount, and the survival rate and the metamorphosis rate of the larvae are improved. By the adoption of the seedling raising method, the seedling raising success rate is remarkably increased, the production cost is low, the method is easy to popularize and apply in seawater pearl shell artificial seedling raising production enterprises, and remarkable social benefits and economic benefits are achieved.

The invention discloses a quality-guaranteed fresh keeping processing method of pinctada martensii meat, and belongs to the technical field of processing of marine products. The quality-guaranteed fresh keeping processing method of pinctada martensii meat comprises the working procedures of performing soaking and cleaning in seawater, removing surface mucosa, performing fishy smell removal treatment, performing sterilization treatment, performing precooling treatment, performing soaking treatment in quality-guaranteed fresh keeping liquid, performing vacuum packing and the like. The quality-guaranteed fresh keeping processing method of pinctada martensii meat provided by the invention can perform quality-guaranteed fresh keeping processing on the pinctada martensii meat, people in inland remote districts can taste the primary taste of the pinctada martensii meat, nutrients and moisture can be guaranteed not to flow out, at the premise that mouth feel is guaranteed, storage time can beprolonged, the haul distance can be enlarged, the supply cycle of seafood can be prolonged, and the transport cost can be saved. In the processing treatment course, the quality-guaranteed fresh keeping processing method is free from any materials harmful to human bodies, and the eating safety of the pinctada martensii meat can be guaranteed.

The invention discloses a staged low-specific-gravity mariculture method for pinctada martensii pearl culturing shellfish. The method comprises the steps as follows: selecting a culture pond, treating the culture pond, culturing the pearl culturing shellfish, harvesting pearls and the like, and the method specifically comprises the following steps: cleaning the pearl culturing shellfish which is cultured for 7-8 months in a sea area and is 3-4 months before pearl harvesting, and transferring the pearl culturing shellfish to the culture pond for hanging culture. The specific gravity of seawater in a culture water body is reduced, rapid growth and reproduction of unicellular algae in the water body are promoted through low-specific-gravity seawater, sufficient bait supply is provided for growth of the pinctada martensii, growth of the pinctada martensii is promoted, and the nacre secretion speed of the pinctada martensii is increased so as to increase the thickness of a nacre layer; and the pinctada martensii pearls produced with the culture method have large pearl layer thickness, the economic benefits of pinctada martensii pearl culture can be increased, and the staged low-specific-gravity mariculture method for the pinctada martensii pearl culturing shellfish is easily popularized and applied in pearl production enterprises and farmers and has remarkable social benefits and economic benefits.

The invention provides application of pinctada martensii mucus to extraction of exosomes, the exosomes and an extraction method and application of the exosomes. According to the method, the vesicular nanoparticles are separated from the pinctada martensii mucus by adopting an ultracentrifugation method and are identified as the exosome, so that reference is provided for extracting the bioactive exosome from marine organisms, and meanwhile, the utilization value of the pinctada martensii is also improved. The exosome extracted from the pinctada martensii mucus can enhance the cell viability, reduce the ROS level in cells, inhibit the activation of NF-kappa B and NLRP3 pathways and reduce the expression level of related inflammatory factors mRNA, and has a certain anti-inflammatory effect.

The invention relates to a culture method of luminous pearl. The culture method comprises the following steps: selecting immature and healthy seawater pinctada martensi or freshwater hyriopsis cumingii of one year's old as a mother shell; cutting down cell slices from the pinctada martensi or hyriopsis cumingii; taking fluorite granules ground into round as pearl nucleus; implanting the fluorite granules and the cell slices as pearl nucleuses in the stomach of the mother shell; culturing the mother shell with seawater or freshwater for 7-9 months to form nacre on the pearl nucleus, and takingout the mother shell to obtain the luminous pearl. Fluorite is used as the pearl nucleus, the cultured pearl can shine in a dark place, the cultured pearl has high hardness and is not easy to wear, the product gloss is high, bleaching and brightening tretament is not required, and the luminous pearl is good in environmental protection performance, harmless to the human body and suitable for wearing.

The invention belongs to the technical field of genetic engineering, and particularly discloses pinctada martensi lectin PmCLEC-1 and application thereof. The amino acid sequence of the pinctada martensi lectin PmCLEC-1 is as shown in the SEQ ID NO:1; and the nucleotide sequence of the pinctada martensi lectin PmCLEC-1 is as shown in the SEQ ID NO:2 or 3. Furthermore, the pinctada martensi lectin PmCLEC-1 has an effect of inhibiting growth of colibacillus and micrococcus luteus, and has excellent application prospects.

The invention discloses a method for producing shell-attached pearls and regenerative cell pearls by using pinctada martensii golden lip system pearl culture shellfish. When pearls of the pinctada martensii golden lip system pearl culture shellfish are harvested, pearl culture shellfish which are large in size, regular in shell shape, full in visceral mass and good in growth in the same batch of pearl culture shellfish are selected, the nucleus-reserved shellfish is inspected through an X-ray machine and subjected to anesthesia treatment, free pearls meeting the quality requirement are suckedout through a minimally invasive operation, and pearl sacs are stored; regenerated cell pears are generated by utilizing the preserved pearl sac secreted nacres, and simultaneously to be pasted on a shell-attached pearl model to produce shell-attached pearls; the shell-attached pearl model is directly attached to produce shell-attached pearls after anesthesia treatment of the enucleated shells, the pearl-cultured shells are returned into a sea area for culturing pearls after operation, and harvesting of the shell-attached pearls and regenerated cell pearls / or the shell-attached pearls is realized after culturing for 12-15 months. According to the method, the utilization rate of pearl culture of pinctada martensii can be increased, and the economic benefit of pearl culture is increased.

The invention relates to the technical field of pearl cultivation, in particular to a novel method for cultivating large free pearls by pinctada maxima. The novel method mainly comprises the following steps of: cutting off 30-40% of retractor of pinctada maxima pearl cultivating shells, reducing the extrusion strength of the retractor on pearl nucleuses, implanting only one perfect circular pearl nucleus at a left bag nucleus position for the first nucleus implantation, and during the second and third nucleus implantation, implanting one pearl nucleus at the left bag nucleus position and a right bag nucleus position respectively. The pinctada maxima belongs to rare protected species, in order to make full use of the pearl cultivation potential of the pinctada maxima, nucleus implantation is carried out three times in total, and meanwhile, the diameter of harvested pearls is 2.0-3.0 mm larger than that of pearls obtained in the previous step every time nucleus implantation is added; and by adopting the nucleus implantation cultivation method, the pearl cultivation capacity of the pinctada maxima is utilized to the maximum extent, the survival rate, the nucleus remaining rate and the superior pearl rate of the pinctada maxima are increased, and large free pearl industrial cultivation of the pinctada maxima can be achieved.

The invention discloses a cultivation method of golden yellow seawater pearls. The cultivation method comprises the following steps that S1, pearl shells with yellow inner side layers of shells are selected and mantle cell small pieces are obtained; S2, pinctada martensii of which the inner side layer of the shell is yellow is selected, the pearl nucleus is implanted into the pinctada martensii through an operation, and the obtained mantle cell small pieces are attached to the pearl nucleuses; S3, the pinctada martensii after the operation is put into a seawater pond for recuperation for 25-35days; dead shellfishes are removed, attachments of live shellfishes are removed, and then cultivation is continued; S4, after culturing for 2-3 months, the dead shellfishes are removed and the attachments are cleaned, and then cultivation is continued; the steps are repeated for 4-5 times to complete the cultivation process. The pearl shells with yellow inner side surface layers are selected to obtain the mantle cell small pieces, the mantle cell small pieces and the pearl nucleuses are implanted into the nucleus positions of the pinctada martensii with yellow inner side surface layers through cell transplantation, in the cultivation process, pearl sacs are formed through endocrine decomposition and melting of the pinctada martensii, and golden yellow nacre layers are formed, and therefore the golden yellow pearls are produced.

The invention belongs to the technical field of cell culture, and discloses a pinctada maxima mantle cell in-vitro culture solution, a preparation method and a use method. The cell in-vitro culture solution comprises the following components: a basic cell culture medium, an isatis root extracting solution, a honeysuckle extracting solution, fetal calf serum, pearl oyster body fluid, kanamycin, a balanced salt solution and glucose. According to the invention, Chinese herbal medicines such as isatis root and honeysuckle, pearl oyster body fluid and other components are added into a basic cell culture medium, and mantle cell culture and in-vitro culture are realized through optimal proportioning of the components. The added isatis root extracting solution and honeysuckle extracting solution play a role in inhibiting harmful bacteria in the external environment in the whole culture process, and meanwhile, the growth life of cells is prolonged.

The method for cultivating 3D Baroque pearls of the present invention is characterized in that it comprises the following steps: cutting the mantle tissue cell sheets of river mussels or mother-of-pearl oysters for tissue culture to provide implanted cell sheets for cultivating pearls; The pearl oyster is used as the female parent of pearl; adopt the method of "combined modeling of multiple implanted cell sheets" or "combined modeling of multiple implanted cell sheets and bead nuclei", and implant the pearl breeding position of the female parent through surgery. Then put the postoperative mother pearls back into the water to cultivate pearls, and the pearls produced are 3D Baroque pearls with nuclei or no nuclei. The 3D baroque pearl is a personalized pearl product with uneven surface, 3D shape inside and outside, and irregular outline. All kinds of high-end jewelry.

The invention discloses a pinctada martensii pearl cultivation shellfish recuperation method, and belongs to the technical field of aquaculture. The method comprises the following steps: transferring pearl shells subjected to a nucleus implantation operation to a recuperation pool for cultivation, keeping microwave inflation, starting to feed from the third day after the operation, feeding in the morning and evening every day, feeding chrysophyceae and microcapsule feed as food for the pearl shells, and feeding vitamin D and lysine hydrochloride as nutrition additives, wherein the cultivation density of the pearl shells is 100-120 / m < 3 >, and the hanging water depth is 1.0-1.2 m; in the nucleus implanting and pearl culturing process of the pinctada martensii, seawater filtered by a fine sand layer and shell powder is used as postoperative recuperation water for nucleus implanting of the pinctada martensii, and the specific gravity of the seawater is set to be 1.020-1.025; by adopting the method for post-operation recuperation of the Pinctada martensii, the survival rate of the Pinctada martensii is high, the nucleus reserving and pearl forming rate is high, and the method is suitable for post-operation recuperation of the seawater Pinctada martensii, easy to popularize and apply in production enterprises and good in economic benefit and social benefit.

The invention relates to a 3D multi-nucleus special-shaped pearl and a culture method thereof. The 3D multi-nucleus special-shaped pearl comprises a 3D modeling nucleus and a pearl outer layer, wherein the 3D modeling nucleus is formed by arranging a plurality of pearl nucleuses, and the pearl outer layer wraps the nucleuses. The culture method comprises the following steps of cutting mantle tissue cell sheets of freshwater mussels or pinctada martensii, and providing cell sheets for tissue culture to form nucleus implantation and pearl breeding or directly utilizing the cell sheets for nucleus implantation and pearl breeding; selecting pearl breeding mussels or pearl breeding shellfishes as pearl breeding parents; and adopting an implantation manner of 'combined modeling of the plurality of cell sheets and the plurality of pearl nucleuses' or 'combined modeling of the single cell sheet and the plurality of pearl nucleuses', implanting the cell sheets and the pearl nucleuses into pearl breeding positions of the pearl breeding parents, then putting the pearl breeding parents back into water to breed and breed pearls, and enabling produced pearls to be the 3D multi-nucleus special-shaped pearl. The 3D multi-nucleus special-shaped pearl is a personalized pearl product with an uneven surfaces, a multi-nucleus 3D form in the surfaces and the interior and an irregular outline, and can be widely applied to various design creation and inlaying into various special jewelries.

The invention discloses cDNA of antimicrobial peptide gene AP from pinctada fucata, the nucleotide sequence is as shown in SEQ ID NO. 1, and the amino acid sequence can be deduced to be as shown in SEQ ID NO.2. The invention further discloses an expression vector containing the antimicrobial peptide gene from the pinctada fucata and a recombinant microbe transformed by utilizing the vector, discloses a method for preparing the antimicrobial peptide gene from the pinctada fucata by utilizing the microbe, and simultaneously discloses an application of the antimicrobial peptide gene from the pinctada fucata in the preparation of drugs for treating or preventing sea water pearl oyster type anti-gram-negative bacteria. The invention adopts the genetic engineering technology for producing recombinant antimicrobial peptide protein from the pinctada fucata, and the recombinant antimicrobial peptide protein is utilized for preparing sea water pearl oyster antibacterial agents or fungicides.

The invention belongs to the technical field of cell culture, and discloses a pinctada fucata pallium cell in-vitro culture solution, a preparation method and a use method, and the cell in-vitro culture solution comprises the following components: a basic cell culture medium, tea polyphenol, fetal calf serum, pearl oyster body fluid, kanamycin, a balanced salt solution and glucose. The Chinese herbal medicine extract tea polyphenol, pearl oyster body fluid and other components are added into the basic cell culture medium, the purposes of bacteriostasis, oxidation resistance and cell aging resistance are achieved in a reasonable proportion, and the pearl oyster body fluid culture medium has the advantages of being free of toxic and side effects, not prone to causing environmental pollution and free of residues; and the survival rate of mantle cell culture is increased, the operation cost is reduced, the operation difficulty is reduced, and the bottleneck of mantle cell in-vitro culture is broken through.

The invention discloses a method for cultivating black pearls by autotransplantation of pinctada palustris, which comprises the following steps: selecting pinctada palustris, performing anesthesia treatment on the pinctada palustris before an operation, performing a nucleus implantation operation, recuperating the pinctada palustris, cultivating and managing the pinctada palustris and the like, and comprises the following steps: selecting healthy pinctada palustris with a pearl layer with strong luster on the inner surface of the pinctada palustris as the nucleus implantation pinctada palustris; the method comprises the following steps of: anesthetizing the pearl shells before a nucleus implanting operation, taking down a mantle small piece by using a special tool, implanting the taken-down mantle small piece and pearl nucleuses into connective tissues of the pearl shells with the small pieces taken, and then recuperating the pearl shells after the operation in a fish pond and cultivating the pearl shells in a pearl breeding sea area in sequence. By adopting the method to cultivate black pearls through nucleus implantation of the pinctada shells, the survival rate of the pinctada shells is high, the nucleus-remaining pearl-forming rate is high, the nacre secretion speed is high, the pearl luster is strong, the method is suitable for artificial pearl cultivation production of the pinctada shells, the method is easy to popularize and apply in pearl production enterprises and farmers, and the social benefit and the economic benefit are good.

The invention discloses a seedling collecting method for treating pinctada maxima attached seedlings through high-specific-gravity seawater soaking, and belongs to the technical field of aquaculture. The method comprises the steps of water storage container preparation, high-specific-gravity seawater preparation, high-specific-gravity soaking seawater preparation, attached seedling soaking treatment, seedling collection treatment and seedling culture. According to the method provided by the invention, the high-specific-gravity seawater is adopted to soak and treat the seedlings of the pinctada maxima attached to a seedling collector, the seedlings fall off automatically, damage to the seedlings during manual seedling collection is reduced, the breeding survival rate of the seedlings of the pinctada maxima is increased, and the method is easily popularized and applied in pinctada maxima seedling production units and has good economic benefits and ecological benefits.

The invention relates to the field of commodity manufacturing, and especially relates to a production method and a production apparatus of negative hydrogen ion water. The production method of negative hydrogen ion water comprises the following steps: mixing 30-60 wt% of zeolite, 20-45 wt% of clay for chinaware, 1-25 wt% of mother pearl shellfishes, 2-35 wt% of coral calcium and 10-30 wt% of ferric oxide, and carrying out high temperature oxidation sintering to form ceramic balls, carrying out high temperature reduction sintering in a hydrogen and nitrogen mixed gas with the hydrogen / nitrogen volume ratio of 1:9 to form ceramic balls containing CaH2 and Fe3O4, carrying out short distance irradiation on the ceramic balls containing CaH2 and Fe3O4 by using magnets with the magnetic induction intensity of 2T to make the ceramic balls have magnetic force, and placing the ceramic balls having magnetic force in water to form negative hydrogen ions (H<->). The production apparatus of negative hydrogen ion water comprises a silt filter, a carbon block filter, an antibacterial filter and a negative hydrogen ion generator which are sequentially connected from an inlet to an outlet. The apparatus and the method greatly reduce the production cost of negative hydrogen ion products.

The invention discloses a seed production method for improving growth of pinctada martensii with a golden shell color. The method comprises the following steps: (1) selecting female individuals as female parents from a pinctada martensii population with a rapid growth characteristic, performing artificial hybridization by taking pinctada martensii male individuals as male parents, and expanding the population number through self-reproduction in the population; and (2) selecting female individuals with mature gonads from the propagation expanding population obtained in the step (1) as female parents, selecting male individuals with yellow shells from the golden shell color Pinctada martensii strains as male parents, and carrying out artificial fertilization, cultivation and breeding to obtain the improved golden shell color Pinctada martensii. The method can improve the size of the yellow shell individual, can guarantee the shell color purity, and is convenient for production and application.

The invention discloses a movable floating mat deepwater staged breeding method for pinctada palustris, and belongs to the technical field of pinctada palustris breeding, the breeding method comprises the steps of manufacturing a movable floating mat, manufacturing a breeding net bag, carrying out double-layer net bag hanging breeding management on pinctada palustris (young, young, small and middle pinctada palustris), moving the pinctada palustris to a conical cage for breeding when the height of the pinctada palustris reaches 70 mm or above, and the like. The breeding method is suitable for staged deepwater sea area artificial breeding of the pinctada fries, the young pinctada, the small pinctada and the medium pinctada, a stable growth environment can be provided for the breeding period from the fries to the medium pinctada in the sea area, the survival rate and the growth speed are increased, and the method is also easy to popularize and apply in production units and farmers and has wide application prospects. The method has good social benefits, economic benefits and ecological benefits.

The invention discloses a staged low-specific-gravity mariculture method for pearl oyster cultivation of Pinctada martensii, comprising the steps of selecting a culture pond, treating the culture pond, culturing pearl oyster and harvesting pearls, and specifically including cultivating 7-8 in the sea area. Pearl oysters that are 3-4 months away from harvesting pearls are cleaned and moved to the culture pond for hanging culture. By reducing the proportion of seawater in the culture water, the low proportion of seawater is used to promote the rapid growth and reproduction of single-cell algae in the water body. The growth of mother-of-pearl provides sufficient bait supply, promotes the growth of Pinctada martensii, improves the secretion rate of pearl of Pinctada martensii to increase the thickness of nacre; The thickness of the nacre is large, which can improve the economic benefits of Pinctada martensii pearl cultivation. The staged low-specific gravity seawater culture method for Pearl oyster martensii provided by the invention is easy to popularize and apply in pearl production enterprises and farmers. It has significant social and economic benefits.