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Application of Pinctada martensii mucus in extraction of exosome, exosome as well as extraction method and application of exosome

A technology of Pinctada martensii and an extraction method, applied in the biological field, can solve problems such as lack, and achieve the effect of improving the utilization value

Pending Publication Date: 2022-05-13
GUANGDONG OCEAN UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies on the extraction of exosomes from marine organisms are still relatively scarce compared with exosomes from human and plant sources

Method used

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  • Application of Pinctada martensii mucus in extraction of exosome, exosome as well as extraction method and application of exosome
  • Application of Pinctada martensii mucus in extraction of exosome, exosome as well as extraction method and application of exosome
  • Application of Pinctada martensii mucus in extraction of exosome, exosome as well as extraction method and application of exosome

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Embodiment Construction

[0041] Pearl oyster martensii, also known as Hepu pearl oyster, is an important marine cultured shellfish, mainly used for pearl cultivation. Most of the flesh of Pinctada martensii is eaten or discarded. Studies have found that Pinctada martensii flesh contains active ingredients such as anti-oxidation, anti-inflammation, and immunity. Not only that, in addition to the flesh of Pinctada martensii, studies have also found that the mucus of Pinctada martensii contains antioxidant glycoproteins, which greatly improves the development potential of Pinctada martensii and its mucus. In addition, Pinctada martensii also has related applications in the treatment of skin diseases. Peptides isolated from Pinctada martensii have been identified as functioning in promoting wound healing. In addition, pearl extract can treat inflammation and apoptosis of human keratinocytes under ultraviolet irradiation, which indicates that pearl extract has the potential to treat dermatitis.

[0042] ...

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Abstract

The invention provides application of pinctada martensii mucus to extraction of exosomes, the exosomes and an extraction method and application of the exosomes. According to the method, the vesicular nanoparticles are separated from the pinctada martensii mucus by adopting an ultracentrifugation method and are identified as the exosome, so that reference is provided for extracting the bioactive exosome from marine organisms, and meanwhile, the utilization value of the pinctada martensii is also improved. The exosome extracted from the pinctada martensii mucus can enhance the cell viability, reduce the ROS level in cells, inhibit the activation of NF-kappa B and NLRP3 pathways and reduce the expression level of related inflammatory factors mRNA, and has a certain anti-inflammatory effect.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular, to the use of Pinctada martensii mucus for extracting exosomes, exosomes and its extraction method and application. Background technique [0002] Exosomes and extracellular vesicles are spherical nanoparticles composed of lipids, proteins, nucleic acids and other biological factors. At present, there is no clear classification of extracellular vesicles and exosomes in the world. Usually, they are called For vesicular nanoparticles. As a vesicular nanoparticle secreted by cells, exosomes can transmit material information between cells, so exosomes have been used in the treatment of various diseases, such as anti-tumor, anti-inflammation and anti-oxidation. Since exosomes are cell products, the use of exosomes as a therapeutic approach is less cytotoxic and more biocompatible than other therapeutic approaches. [0003] Exosomes are distributed in the body fluids and cells of ma...

Claims

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Application Information

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IPC IPC(8): C12N5/07A61K35/618A61P17/00A61P29/00
CPCC12N5/0601A61K35/618A61P17/00A61P29/00C12N2509/10Y02A50/30
Inventor 李承勇洪鹏志周春霞千忠吉任磊梁燕秋孙省利
Owner GUANGDONG OCEAN UNIVERSITY
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