A kind of culture method of 3D baroque pearl
A culture method and pearl technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of unapplied cultivation pearl production practice, no pearl breeding cases or related reports, and inability to carry out industrialized production, etc., to achieve product High technical content and added value, many types of special shapes, good shape and aesthetic effect
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example 1
[0030] Example 1: Cultivation of 3D Baroque pearls using the mantle of the spinnaker mussel
[0031] First cut out the mantle tissue cell sheet of the spiny mussel, sterilize the cut tissue cell sheet, put it in a petri dish, add an equal amount of culture medium, and then put it into an incubator to cultivate the implanted cell sheet for cultivating pearls. spare. The incubation temperature of the incubator was 25°C and the humidity was 100%. The disinfection solution for tissue cell sheet disinfection is: 1 mg / ml streptomycin, 500 IU / ml penicillin, 100 IU / ml gentamicin and 2 μg / ml nystatin. Culture medium for tissue cell sheet culture: 500 μL medium (10% fetal bovine serum, 900 mg / L D-galactose, 300 mg / L L-glutamine, 550 mg / L sodium pyruvate).
[0032] Select 1-3 year old spinnaker mussels as breeding mother mussels. Before the bead breeding operation, the spinnaker mussels are washed and placed in a relatively clean breeding pond or river water body near the operating ro...
example 2
[0035] Example 2: Cultivation of 3D Baroque pearls with the visceral mass of pleated crowned mussels
[0036] First, cut the mantle tissue cell sheet of Corona rugosa, put the cut tissue cell sheet into a petri dish, add an equal amount of culture medium, and then put it into an incubator to cultivate the implanted cell sheet used for culturing pearls for use. The incubation temperature of the incubator is 25°C-28°C and the humidity is 80%-100%. The disinfectant solution for tissue cell sheet disinfection is: 1 mg / ml streptomycin, 500 IU / ml penicillin, 100 IU / ml gentamicin and 2 μg / ml nystatin. Culture medium for tissue cell sheet culture: 500 μL medium (10% fetal bovine serum, 900 mg / L D-galactose, 300 mg / L L-glutamine, 550 mg / L sodium pyruvate).
[0037] The 1-3-year-old crested mussels were selected as breeding mother mussels. Before the bead breeding operation, rinse the crepe crowned mussels and put them in a relatively clean breeding pond or river water body near the o...
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