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Pinctada maxima mantle cell in-vitro culture solution as well as preparation method and use method thereof

A technology of mantle cells and Pinctada maxima, which is applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve the problems of unreported and incomplete operation, so as to improve the survival rate, reduce the difficulty of culture, The effect of prolonging the growth life

Pending Publication Date: 2021-10-01
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the in vitro culture of mantle cells, the cells often enter the decay stage prematurely, resulting in the inability to complete subsequent operations
[0004] So far, there has been no report on the use of Chinese herbal ingredients to improve the survival rate of cells in vitro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Basal cell culture medium 400ml / L, isatis root extract 90ml / L, honeysuckle extract 120ml / L, fetal bovine serum 4.5ml / L, pearl oyster body fluid 12ml / L, kanamycin 0.05g / L, balanced salt solution 150ml / L L, glucose 223.45ml / L. After being sterilized by ultraviolet light, all the mixtures are evenly mixed, and can be used for in vitro culture of the mantle cells of Pinctada maxima under the condition of ensuring sterility and constant temperature.

Embodiment 2

[0025] Basal cell culture medium 450ml / L, Radix Radix Radix Extract 95ml / L, Honeysuckle Extract 125ml / L, Fetal Bovine Serum 3.5ml / L, Pearl Oyster Body Fluid 11ml / L, Kanamycin 0.05g / L, Balanced Salt Solution 100ml / L L, glucose 215.45ml / L. After being sterilized by ultraviolet light, all the mixtures are evenly mixed, and can be used for in vitro culture of the mantle cells of Pinctada maxima under the condition of ensuring sterility and constant temperature.

Embodiment 3

[0027] Basal cell culture medium 353.42ml / L, isatidis root extract 100ml / L, honeysuckle extract 130ml / L, fetal bovine serum 4.5ml / L, pearl oyster body fluid 12ml / L, kanamycin 0.08g / L, balanced salt solution 150ml / L, glucose 250ml / L, add double distilled water to make up to 1L. After being sterilized by ultraviolet light, all the mixtures are evenly mixed, and can be used for in vitro culture of the mantle cells of Pinctada maxima under the condition of ensuring sterility and constant temperature.

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Abstract

The invention belongs to the technical field of cell culture, and discloses a pinctada maxima mantle cell in-vitro culture solution, a preparation method and a use method. The cell in-vitro culture solution comprises the following components: a basic cell culture medium, an isatis root extracting solution, a honeysuckle extracting solution, fetal calf serum, pearl oyster body fluid, kanamycin, a balanced salt solution and glucose. According to the invention, Chinese herbal medicines such as isatis root and honeysuckle, pearl oyster body fluid and other components are added into a basic cell culture medium, and mantle cell culture and in-vitro culture are realized through optimal proportioning of the components. The added isatis root extracting solution and honeysuckle extracting solution play a role in inhibiting harmful bacteria in the external environment in the whole culture process, and meanwhile, the growth life of cells is prolonged.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to an in vitro culture solution, a preparation method and a use method of mantle cells of Pinctada maxima. Background technique [0002] Pinctada maxima jameson (Pinctada maxima jameson), also known as white butterfly shell, belongs to Mollusca, Lamellibranchia, Pterioida, Pteriidate, Pearl oyster in terms of animal taxonomy. Pinctada is the largest marine pearl oyster. Large pearl oysters are mainly distributed in the South Pacific and Western Pacific waters. In my country, they are mainly distributed in Hainan, Leizhou Peninsula, Xisha Islands and other waters; abroad, they are distributed in the coastal waters of Southeast Asian countries. Since the 1960s, my country has successfully carried out the scientific research on the key technology of artificial seedling cultivation and intercalation of Pearl Oyster oyster, which has promoted the rapid development of se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07
CPCC12N5/0601C12N2500/76C12N2500/82C12N2500/34
Inventor 方伟陈明强李有宁王雨于刚
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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