45 results about "Rat Bone Marrow" patented technology

The invention discloses a magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 and a preparation method thereof. The method for preparing the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 comprises the steps of: firstly preparing hydrophobic monodisperse Fe3O4 nano-particles by adopting a chemical oil-phase high-temperature method, and modifying the surfaces of the hydrophobic Fe3O4 nano-particles to ensure that the hydrophobic Fe3O4 nano-particles are dispersed in a water phase; preparing luminescent CdTe quantum dots of which the surfaces are provided with carboxyl groups, and precipitating the luminescent CdTe quantum dots on the surfaces of the magnetic Fe3O4 nano-particles through the co-precipitation; then utilizing ligand exchange to modify a silane coupling agent on the surfaces of the luminescent CdTe quantum dots; and finally forming an outermost SiO2 coating layer through silane or silicon ester hydrolysis. The diameter of the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 is between 30 and 50nm; the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2has double functions of magnetism and fluorescence at the same time, has strong and durable fluorescence intensity after labeling rat bone marrow-derived mesenchymal stem cells, and apparently reduces cellular magnetic resonance signals. The particle has broad application prospect in the fields such as biological labeling, bioseparation and the like.

Cellulose and sulfated cellulose fibrous meshes exhibiting robust structural and mechanical integrity in water were fabricated using a combination of electrospinning, thermal-mechanical annealing and chemical modifications. The sulfated fibrous mesh exhibited higher retention capacity for human recombinant bone morphogenetic protein-2 than the cellulose mesh, and the retained proteins remained biologically active for at least 7 days. The sulfated fibrous mesh also more readily supported the attachment and osteogenic differentiation of rat bone marrow stromal cells in the absence of osteogenic growth factors. These properties combined make the sulfated cellulose fibrous mesh a promising bone tissue engineering scaffold.

Intravenous administration of bone marrow cells collected from rat bone marrow or peripheral blood to a rat cerebral infarction model was found to be effective in treating cerebral infarction. Human and murine bone marrow stem cells showed similar effects. Mesenchymal cells such as bone marrow cells, cord blood cells, or peripheral blood cells can be used as agents for in vivo administration against cranial nerve diseases.

The invention discloses a method of induced differentiation of rat bone marrow mesenchymal stem cells into adipocytes, which includes: choosing and inoculating third-generation bone marrow mesenchymal stem cells into a cell culture flask according to a concentration of 1 multiplied by 106mL<-1>, and after the cells attaching to a wall grow until cell density reaches 80 percent, adding different concentrations of oleic acid as inducers; according to a volume proportion of 10 percent, adding fetal bovine serum into a basic culture medium DMEM (Dulbecco modified Eagle medium), and respectively adding 25mM of oleic acid, 50mM of oleic acid, 100mM of oleic acid and 200mM of oleic acid as induction culture media for forming adipocytes; replacing the solution once every three days, and with a cell culture flask without a induction culture solution as a control, performing oil red O staining assay after 3 weeks. According to the method, different concentrations of oleic acid can be used to directly induce adipocytes, a novel induction method is established, the configuration of the method is simpler, the induction speed is high, and the induction rate is high. In addition, the method can be used for inducing adipocytes of different sizes and researching the functional differences of the adipocytes of different sizes.

The invention discloses a method for inducing gelation and biomimetic mineralization of a fibroin solution by alkaline phosphatase. According to the invention, the micromolecular polypeptide which issensitive to ALP and has good biocompatibility and self-assembly property is introduced as a gelator precursor; phosphoric acid groups on molecules can be removed under the catalytic action of ALP togenerate NY, supermolecule self-assembly is triggered, and therefore SF co-self-assembly is synergistically induced, and finally SF hydrogel is rapidly formed. ALP wrapped in an SF-NY hydrogel networkstill maintains the catalytic activity of the SF-NY hydrogel network, and free phosphate ions are released by catalyzing beta-glycerophosphate so that formation of apatite minerals is induced in thegel. Due to the mild gelation process and the formation of apatite minerals in the gel matrix, the biomimetic mineralized SF gel can be used as a biomimetic scaffold to promote the adhesion, proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells in vitro, and can also promote the natural healing of femoral defects in a rat model.

The invention relates to a method for extracting mesenchymal stem cells. The method includes the steps: performing pretreatment to obtain a thigh bone and a shin bone; flushing the thigh bone and theshin bone, extracting marrow cavity contents to obtain flushing liquid containing crumby marrow cavity contents; and collecting stem cells in the flushing liquid; blowing and beating remaining crumbymarrow cavity contents to obtain single-cell suspensions, and collecting stem cells in the single-cell suspensions; grafting and culturing the acquired stem cells. The method can be used for extracting mesenchymal stem cells of rats, and the acquired mesenchymal stem cells are applied to research of cell biology. The method has the advantages of simplicity in operation, low cost, pollution probability and stem cell loss, more cell extraction amount, high purity, good repeatability and the like, and the method is of great significance for biological research in the field.

The invention discloses a rat bone marrow tumour cell NSO and a preparation method and an application thereof. The rat bone marrow tumour cell NSO can grow in a serum-free and fat-free culture substrate, the preservation number of one strain (NSO-Ch1) is CGMCC No.2127. If the rat bone marrow tumour cell NSO of the invention, which can grow in a serum-free and fat-free culture substrate, is used to express foreign protein, cell culture efficiency and expression efficiency can be ensured, and other animal source ingredients and unsafe factors can be avoided; thus, the invention can effectively lower purification cost and loss and improve the yield and profitability of biological products.

The invention discloses a method for alkaline phosphatase to induce gelation and biomimetic mineralization of silk protein solution. The invention introduces a small molecule polypeptide that is sensitive to ALP and has good biocompatibility and self-assembly properties as a gelling factor The precursor, which can remove the phosphate group on the molecule under the catalysis of ALP to generate NY, triggers supramolecular self-assembly, thereby synergistically inducing SF co-self-assembly, and finally leads to the rapid formation of SF hydrogel. The ALP encapsulated in the SF‑NY hydrogel network still maintains its catalytic activity and induces the formation of apatite minerals in the gel by catalyzing β‑glycerophosphate to release free phosphate ions. Due to the mild gelation process and the formation of apatite minerals in the gel matrix, the biomimetic mineralized SF gel could not only be used as a biomimetic scaffold to promote the adhesion of rat BMSCs in vitro, Proliferation and osteogenic differentiation, and can also promote natural healing of femoral defects in a rat model.

The invention discloses a magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 and a preparation method thereof. The method for preparing the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 comprises the steps of: firstly preparing hydrophobic monodisperse Fe3O4 nano-particles by adopting a chemical oil-phase high-temperature method, and modifying the surfaces of the hydrophobic Fe3O4 nano-particles to ensure that the hydrophobic Fe3O4 nano-particles are dispersed in a water phase; preparing luminescent CdTe quantum dots of which the surfaces are provided with carboxyl groups, and precipitating the luminescent CdTe quantum dots on the surfaces of the magnetic Fe3O4 nano-particles through the co-precipitation; then utilizing ligand exchange to modify a silane coupling agent on the surfaces of the luminescent CdTe quantum dots; and finally forming an outermost SiO2 coating layer through silane or silicon ester hydrolysis. The diameter of the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2 is between 30 and 50nm; the magnetic luminescent composite nano-particle Fe3O4 / CdTe / SiO2has double functions of magnetism and fluorescence at the same time, has strong and durable fluorescence intensity after labeling rat bone marrow-derived mesenchymal stem cells, and apparently reduces cellular magnetic resonance signals. The particle has broad application prospect in the fields such as biological labeling, bioseparation and the like.

The invention relates to the technical field of biological implant materials, in particular to a titanium implant with a nanometer bionic three-dimensional porous titanium trabecula structure and a preparation method and application thereof.The method comprises the steps that in the presence of electrolyte, a titanium substrate M0 is subjected to anodic oxidation, and a titanium substrate M1 with a deposited titanium dioxide nanotube array layer is obtained; removing the titanium dioxide nanotube loaded on the titanium substrate M1 to obtain a titanium substrate M2 loaded with a nano bowl structure; the titanium matrix M2 is placed in an alkaline solution to be subjected to etching treatment, and the titanium implant with the nanometer bionic three-dimensional porous titanium trabecular structure is obtained. The titanium implant with the nano bionic three-dimensional porous titanium trabecula structure prepared on the basis of the method provided by the invention can promote adhesion, proliferation and differentiation capacities of rat bone marrow stromal stem cells in vitro and promote osseointegration of the titanium implant in vivo.

The invention provides a immortal stromal cell systme of rat's marrow and preparation thereof. Immortal stromal cell of rat's marrow can be obtained in short time by the inventive method. The inventive hTERT immortal stromal cell systme of rat's marrow is stable, can be sequentially passed through generations and keep diploid karyotype and characteristics of normal stromal cell of rat's marrow, has extremely strong proliferation ability, and realize immortalization. Immortal stromal cell system of rat's marrow established by the inventive method can conveniently and quickly obtain large amount of stromal cell of rat's marrow with diploid karyotype. This no doubt has great promotion to research of introduction and bone marrow stromal cell in vitro and in vivo.

The invention relates to a method for improving the efficiency of differentiating rat bone marrow mesenchymal stem cells into myocardial cells, which comprises the following steps: (1) constructing alentiviral interference vector G9a-shRNA, and transfecting the G9a-shRNA to the rat bone marrow mesenchymal stem cells; and (2) inducing the bone marrow mesenchymal stem cells transfected with the interference vector through 5-azacytidine, and detecting the positive rate of troponin positive cells by flow cytometry on the 21st day. According to the invention, in the process of inducing the bone marrow mesenchymal stem cells to differentiate into the myocardial cells by the 5-azacytidine, the H3K9me2 methylase G9a is knocked down, and the final induction efficiency is observed, so that a convenient and rapid way is expected to be found in the process of differentiating the bone marrow mesenchymal stem cells into the myocardial cells. According to the method, the rat bone marrow mesenchymalstem cells are transfected by adopting FuGENE (Promega) lipidosome mediated G9a-shRNA lentivirus recombinant plasmids to reduce the expression of H3K9me2, so that the induction efficiency is improved,the verification result is reasonable and accurate, and the experimental process is rigorous and rigorous.

The invention discloses a method for constructing a rat bone mesenchymal stem cells (BMSCs) cell strain for expressing c-met protein through regulation of doxycycline. The method comprises the steps that an rtTA gene lentiviral expression vector is constructed and subjected to transfection into BMSCs so that a BMSCs cell strain (a Tet-On Advanced BMSCs cell strain) for stably and efficiently expressing rtTA protein can be obtained; then, a Len-TRE min CMV-c-met / ALB-GFP lentiviral vector is constructed and subjected to transfection into the Tet-On Advanced BMSCs, and a flow cytometry is used for screening GFP positive BMSCs; and finally the GFP (+) Tet-on-c-met BMSCs cell strain is obtained. The doxycycline can be added into the obtained BMSCs cell strain to induce the obtained BMSCs to express the c-met protein, and the method has important significance for study on blood doping of the cell strain for treating hepatic failures, the directional homing and differentiation capacities of the BMSCs along with the HGF concentration and the dose-effect relationship between the capacities and the HGF / c-met axis expression level.

The invention discloses an SD rat-derived bone marrow mesenchymal stem cell induction medium and an induction method. The induction medium is composed of α-MEM medium and additives. In terms of final concentration, the additives are 5-100ng / ml Composition of transforming growth factor-alpha, 10-200ng / ml regulatory protein beta 1, 100-3000nM trehalose and 50-5000nM dibutyryl cyclic adenosine monophosphate. The SD rat-derived bone marrow mesenchymal stem cell induction medium and induction method of the present invention can significantly increase the secretion of BDNF, HGF, VEGF, GDNF and other factors of rat bone marrow mesenchymal stem cells, and the types of secreted factors are also relatively abundant and It can be secreted stably for a long time; the method of cytokine induction avoids the risks and uncertainties brought about by other methods such as genetic modification, and is more suitable for industrialization and clinical application.

The invention provides a ZIF-8-loaded Janus electrospun fibrous membrane as well as a preparation method and application thereof, and belongs to the technical field of dental restoration materials. The method comprises the following steps: mixing ZIF-8 with a polyvinylpyrrolidone solution, mixing the mixed solution with a poly (epsilon-caprolactone) solution in an isovolumetric manner, and obtaining the Janus electrospun fiber membrane by utilizing a phase separation phenomenon between the polyvinylpyrrolidone solution and the poly (epsilon-caprolactone) solution through a single-shaft electrospinning technology. The invention further provides the Janus electrospun fiber membrane of the ZIF-8, which is obtained by the preparation method. According to the Janus electrospun fibrous membrane, slow release of ZIF-8 is achieved, proliferation of bacteria can be inhibited, osteogenic differentiation of rat bone marrow mesenchymal stem cells is effectively promoted, and the Janus electrospun fibrous membrane has a good treatment effect on periodontal defects caused by periodontitis.

The invention belongs to the technical field of medicine and relates to uses of various traditional Chinese medicine mixed compounds, in particular to application of an effective part group of yang-tonifying recuperating decoction in inducing mesenchymal stem cells to differentiate into nerve cells.The effective part group includes effective parts extracted from medicinal materials, including glucoside compounds, total alkaloids, Angelica sinensis polysaccharide, carthamin yellow and naringenin.Researches show that this effective part group can induce in vitro rat mesenchymal stem cells to differentiate into neuron-like cells and is applicable to drugs for treating nervous system diseases.