46results about How to "Extensive culture conditions" patented technology

Production of gama-polyglutamic acid by fermentation is carried out by: culturing bacillus subtills zju-7 strain, i.e., CGMCC No. 1250, in medium with carbon and nitrogen sources, glutamic acid, MgSO4, NaCl and K2HPO4 to obtain ferment liquid, and making it to pass organic solvent settlement and dialysis. The process is easy, yields highly, and costs low with quality products.

The present invention discloses one kind of Bacilus alcaligenes and its application in preparing Weilan gum. The Bacilus alcaligenes strain has the preservation number of CGMCC No. 1737. The process of preparing Weilan gum with the Bacilus alcaligenes strain includes inoculating the strain to bacteria-free culture medium containing carbon source, nitrogen source, inorganic salt and water; ventilating and stirring culturing in proper conditions; and extracting the fermented liquid to obtain Weilan gum. The Bacilus alcaligenes strain can utilize several kinds of carbon source and nitrogen source and has extensive culture conditions, and the process is simple and has high Weilan gum yield, high Weilan gum quality and low production cost.

The invention discloses application of Bacillus subtilis in high-yield adenosine and acetoin co-production. The Bacillus subtilis is CGMCC No. 4484. Screened strains can be fermented for adenosine production and acetoin co-production by multiple carbon sources and nitrogen sources. Yield of adenosine and acetoin can be evidently increased by adding sodium citrate into fermentation medium. Operation is convenient and simple, culture conditions are extensive, a higher level can be kept on a 5L fermentation tank, and potential of large-scale industrialization is provided.

The invention discloses bacillus subtilis. According to the bacillus subtilis, the category name is bacillus subtilis PG-8, the bacillus subtilis is collected at China general microbiological culture collection center (CGMCC), the registered number is CGMCC NO. 6324, and the collection data is 10, July, 2012. The invention further discloses application of the bacillus subtilis in preparation of gamma-D-polyglutamic acid. The bacillus subtilis is cultured in a culture medium containing L-glutamic acid only, and then, the gamma-D-polyglutamic acid of 30-60 g / L can be accumulated under optimized conditions. According to the bacillus subtilis, a variety of carbon and nitrogen sources can be utilized, culture conditions are extensive, the operation is convenient and simple, the yield of the gamma-D-polyglutamic acid is high, and the production cost is low, so that the bacillus subtilis can be widely applied to the fields of plant water conservation, food additives and the like.

The invention relates to a cellulomonas fimi DA 8 bacterial strain as well as an acquiring method and application thereof. The bacterial strain has the capability of producing pectinase and hemicellulase and has flax degumming activity. The cellulomonas fimi bacterial strain screened by the method has the advantages of large enzyme yield, complete enzyme system, no toxicity, rough culturing condition and the like; after used for flax degumming, the composite degumming enzyme solution not only greatly shortens a degumming period, but also reduces the cost, the fiber splitting degree is improved, and the degumming efficiency reaches 95%. The degumming method requires basically the same equipment required in the traditional warm water degumming method so that the method is especially suitable for improving a process on the basis of the traditional equipment in old enterprises; the cellulomonas fimi bacterial strain screened with the method not only can be used for flax raw-stem degumming, roving scouring, flax fabric biological pretreatment, but also can be used for bast fiber degumming and biological pretreatment of bast fiber plants, such as ramie, jute, hemp and the like.