A method for coagulation treatment of mining wastewater with microbial flocculant and surfactant
A technology of microbial flocculants and surfactants, applied in the field of wastewater treatment, can solve the problems of large amount of microbial flocculants and long flocculation time, and achieve good treatment effect, extensive culture conditions, and increase the effect of settling speed.
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Embodiment 1
[0029] Prepare simulated wastewater: prepare a suspension of 30 g / L with pure quartz minerals with a particle size of less than 0.074 mm, and the pH value is 7.1. The negatively charged quartz on the surface is difficult to settle naturally due to electrostatic repulsion.
[0030] The specific steps for treating wastewater are as follows:
[0031] ⑴ Silicate bacteria are used as strains, and the strains are inoculated into 100 mL of liquid medium (sucrose 5.0 g / L, Na 2 HPO 4 2.0 g / L, MgSO 4 •7H 2 O 0.5 g / L, FeCl 3 0.005 g / L, CaCl 2 0.1 g / L, kaolin 0.5g / L) in a 250 mL Erlenmeyer flask, adjust the pH of the medium to 7.3, culture in a shaker at 150 r / min for 72 h to obtain a silicate bacteria culture solution;
[0032] The culture solution was centrifuged at 6000 r / min for 20 min, then added twice the volume of pre-cooled ethanol and mixed until a flocculent precipitate was formed, centrifuged at 3000 r / min for 15 min, washed three times with pre-cooled ethanol, and agai...
Embodiment 2
[0037]Wastewater to be treated: the concentration of slime particles in the slime water is 34g / L, the pH value of the slime water is 8.3, the concentration of suspended solids in the slime water is high, the content of fine particles is high, the ash content is low, and natural settlement is difficult. The particle size and composition analysis are as follows:
[0038]
[0039] The specific steps for treating wastewater are as follows:
[0040] ⑴ Silicate bacteria are used as strains, and the strains are inoculated into 100 mL of liquid medium (sucrose 6.0 g / L, Na 2 HPO 4 3.0 g / L, MgSO 4 •7H 2 O 0.7 g / L, FeCl 3 0.005 g / L, CaCl 2 0.2 g / L, kaolin 0.25 g / L) in a 250 mL Erlenmeyer flask, adjust the pH of the medium to 6.5, and culture in a shaker at 200 r / min for 96 h to obtain a silicate bacteria culture solution. After centrifuging at 5000 r / min for 30 min, add twice the volume of pre-cooled ethanol and mix until flocculent precipitates are formed, centrifuge at 4000...
Embodiment 3
[0045] Preparation of simulated wastewater: kaolin with a particle size of less than 0.045mm accounting for 83% was prepared into a 10 g / L suspension with a pH value of 6.8. Natural settlement of such clay mineral suspensions is difficult.
[0046] The specific steps for treating wastewater are as follows:
[0047] (1) Silicate bacteria are used as strains, and the strains are inoculated into 100 mL of liquid medium (sucrose 4.0 g / L, Na 2 HPO 4 1.5g / L, MgSO 4 •7H 2 O 0.5g / L, FeCl 3 0.008 g / L, CaCl 2 0.15 g / L, kaolin 0.70 g / L) in a 250 mL Erlenmeyer flask, adjust the pH of the medium to 7.3, and culture it in a shaker at 180 r / min for 72 h to obtain a silicate bacteria culture solution. After centrifuging at 6000 r / min for 30 min, add twice the volume of pre-cooled ethanol and mix until flocculent precipitates are formed, centrifuge at 2000 r / min for 25 min, wash with pre-cooled ethanol three times, and centrifuge again at 4000 r / min After 50 minutes, vacuum-dry at 42°...
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