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Bacillus and its use of preparation of gama-polycysteine

A technology of Bacillus subtilis and polyglutamic acid, applied in bacteria, fermentation and other directions, can solve the problems of long production cycle, high consumption of raw materials and high production cost, and achieves optimization of culture conditions, good moisture absorption performance and moisturizing performance, high efficiency The effect of inexpensive preparation

Inactive Publication Date: 2005-07-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the fermentative production of γ-PGA has made great progress, there are still problems of consuming more raw materials, longer production cycle, low production efficiency and higher production cost. Therefore, efficient and low-cost production of γ-PGA still remains pending further study

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Bacillus subtilis (Bacillus subtills) zju-7 was activated on a slant at 37°C, and the slant medium: glucose 1.5%, peptone 1%, glutamic acid 3%, MgSO 4 0.1%, NaCl 1%, K 2 HPO 4 0.01%, activated for 8 hours.

[0043] Shake flask medium: glucose 2%, peptone 1.5%, glutamic acid 3%, MgSO 4 0.1%, NaCl 1%, K 2 HPO 4 0.01%, pH7, filling volume 50ml / 500ml shake flask, sterilized at 115℃ for 20 minutes.

[0044] After the sterilization, cool down, inoculate the above-mentioned Bacillus subtilis (Bacillus subtills) zju-7, the inoculum amount is 3%, culture in a shake flask at 37° C. with a rotation speed of 200 rpm, carry out a shake flask fermentation experiment under the above conditions, and cultivate for 24 hours.

[0045] After the fermentation, the bacteria in the fermentation broth were removed by centrifugation, the pH value of the supernatant was adjusted to 4 with hydrochloric acid, and then 4 times the volume of methanol was added for precipitation. Redissolve...

Embodiment 2

[0047] Activate Bacillus subtills (Bacillus subtills) zju-7 CGMCC No.1250 on the slant medium, as in Example 1;

[0048] Shake flask medium: glucose 2%, peptone 2%, glutamic acid 5%, MgSO 4 0.2%, NaCl 2%, K 2 HPO 4 0.05%, pH7, liquid volume 30 / 250ml shake flask, sterilized at 115°C for 20 minutes. After sterilization, cool and inoculate, the inoculum amount is 3%, the strain is Bacillus subtills (Bacillus subtills) zju-7, cultivated at 37° C. for 24 hours, and the rotation speed of the shaker flask is 200 rpm.

[0049] After the fermentation was finished, the gamma-polyglutamic acid was separated and purified as in Example 1, and the yield could reach 18 g / L.

Embodiment 3

[0051] Activate Bacillus subtills (Bacillus subtills) zju-7 CGMCC No.1250 on the slant medium, as in Example 1;

[0052] Glucose 2%, peptone 2%, glutamic acid 8%, MgSO 4 0.1%, NaCl 2%, K 2 HPO 4 0.05% is shake flask culture medium, pH 7, liquid volume 30ml / 200ml shake flask, sterilized at 115°C for 20 minutes, after sterilization, cool down, inoculate Bacillus subtilis (Bacillus subtills) zju-7, inoculum volume is 3% , cultured in shake flasks at 37°C, 200rpm, cultivated for 24 hours,

[0053] After the fermentation, the γ-polyglutamic acid was isolated and purified according to the method of Example 1, and its yield could reach 23g / L.

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Abstract

Production of gama-polyglutamic acid by fermentation is carried out by: culturing bacillus subtills zju-7 strain, i.e., CGMCC No. 1250, in medium with carbon and nitrogen sources, glutamic acid, MgSO4, NaCl and K2HPO4 to obtain ferment liquid, and making it to pass organic solvent settlement and dialysis. The process is easy, yields highly, and costs low with quality products.

Description

technical field [0001] The invention relates to a high-yield gamma-polyglutamic acid (gamma-PGA) microbial strain and a method for preparing gamma-polyglutamic acid. Background technique [0002] γ-polyglutamic acid (Poly γ-glumatic acid, γ-PGA) is a homo-amino acid compound that can be synthesized by microorganisms. As a biopolymer material, γ-PGA has the advantages of good biodegradability, edible, non-toxic to human body and environment. Therefore, γ-PGA and its derivatives are widely used in food, cosmetics, medicine and water treatment. [0003] At present, the methods for synthesizing γ-PGA mainly include chemical synthesis, extraction and microbial synthesis. However, the chemical synthesis method has a long synthesis route, many by-products, low yield, and the product has a small molecular weight, which is difficult to meet the requirements as a new type of drug carrier material, and has no industrial application value. However, due to the low concentration of γ-P...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/00
Inventor 徐志南石峰岑沛霖
Owner ZHEJIANG UNIV
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