Method for coagulation treatment of mining industry wastewater by means of microbial flocculant and surfactant
A microbial flocculant and surfactant technology, which is applied in the field of wastewater treatment, can solve the problems of long flocculation time and large amount of microbial flocculant, and achieve the effects of extensive culture conditions, good treatment effect, and shortened flocculation time
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Embodiment 1
[0029] Preparation of simulated wastewater: prepare a suspension of 30g / L with pure quartz minerals with a particle size of less than 0.074mm, the pH value is 7.1, and the negatively charged quartz on the surface is difficult to settle naturally due to electrostatic repulsion.
[0030] The specific steps for treating wastewater are as follows:
[0031] (1) Silicate bacteria are used as strains, and the strains are inoculated into 100mL liquid medium (sucrose 5.0g / L, Na 2 HPO 4 2.0g / L, MgSO 4 ·7H 2 O0.5g / L, FeCl 3 0.005g / L, CaCl 2 0.1g / L, kaolin 0.5g / L) in a 250mL Erlenmeyer flask, adjust the pH of the medium to 7.3, culture in a shaker at 150r / min for 72h to obtain a silicate bacteria culture solution;
[0032] Centrifuge the culture solution at a centrifugal rate of 6000r / min for 20min, add twice the volume of pre-cooled ethanol and mix until flocculent precipitates are formed, centrifuge at a centrifugal rate of 3000r / min for 15min, wash with pre-cooled ethanol three ti...
Embodiment 2
[0037] Wastewater to be treated: the concentration of slime particles in the slime water is 34g / L, the pH value of the slime water is 8.3, the concentration of suspended solids in the slime water is high, the content of fine particles is high, the ash content is low, and natural settlement is difficult. The particle size and composition analysis are as follows:
[0038]
[0039] The specific steps for treating wastewater are as follows:
[0040] (1) Silicate bacteria are used as strains, and the strains are inoculated into 100mL liquid medium (sucrose 6.0g / L, Na 2 HPO 4 3.0g / L, MgSO 4 ·7H 2 O0.7g / L, FeCl 3 0.005g / L, CaCl 2 0.2g / L, kaolin 0.25g / L) in a 250mL Erlenmeyer flask, adjust the pH of the medium to 6.5, culture in a shaker at 200r / min for 96h to obtain a silicate bacteria culture solution, and the culture solution is centrifuged at a speed of 5000r / min After centrifugation for 30 minutes, add twice the volume of pre-cooled ethanol and mix until flocculent precipi...
Embodiment 3
[0045] Prepare simulated wastewater: prepare a 10g / L suspension with kaolin with a particle size of less than 0.045mm accounting for 83%, and a pH value of 6.8. This type of clay mineral suspension is difficult to settle naturally.
[0046] The specific steps for treating wastewater are as follows:
[0047] (1) Silicate bacteria are used as strains, and the strains are inoculated into 100mL liquid culture medium (sucrose 4.0g / L, Na 2 HPO 4 1.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeCl 3 0.008g / L, CaCl 2 0.15g / L, kaolin 0.70g / L) in a 250mL Erlenmeyer flask, adjust the pH of the medium to 7.3, and obtain a silicate bacteria culture solution after culturing in a 180r / min shaker for 72h, and the culture solution is centrifuged at a rate of 6000r / min After centrifuging for 30 minutes, add twice the volume of pre-cooled ethanol and mix until flocculent precipitates form. Centrifuge at a centrifugal rate of 2000r / min for 25 minutes, wash with pre-cooled ethanol three times, and centrifuge ...
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