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Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof

A technology of Pseudomonas fluorescens and strains, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as no reports on the application of hemp fiber glial decomposition, and shorten the degumming period and culture. The conditions are extensive and the effect is conducive to promotion

Inactive Publication Date: 2012-07-25
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are relevant patents on Pseudomonas fluorescens and its fermentation and cultivation method, it is mostly used to promote crop growth and prevent and control plant soil-borne diseases (CN200510112542.9), but it has not been seen in the decomposition of hemp fibers such as flax. application report

Method used

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  • Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof
  • Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof
  • Fluorescent pseudomonad DA4 strain as well as acquisition method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Screening and Obtaining of Flax Degumming Strains

[0045] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 37°C for 1 to 4 days, obtained from the wild-type degumming dominant strain;

[0046] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of sterile water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g,K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 9 with NaOH;

[0047] (2) Inoculate 4 loops of the bacterial strain obtained in step (1) into pectin nutrient medium (petri dish diameter 10cm), cultivate at 28°C for 72h, after the formation of colo...

Embodiment 2

[0055] In order to enable the strain to maintain a stable high-yielding characteristic, beef extract peptone medium is used as the slant preservation medium. The raw materials and preparation methods of the medium are: 0.5g beef extract, 1g peptone, and 0.5g sodium chloride (pH7.4-7.6) , add water to 100ml constant volume, culture overnight at 37°C 200rpm, add 100ml of freezing buffer; among them, the freezing buffer consists of 0.0627g of potassium dihydrogen phosphate, 0.0177g of dipotassium hydrogen phosphate, 0.0588g of sodium citrate, sulfuric acid heptahydrate Magnesium 0.02645g, glycerin 10ml, add water to volume to 100ml; divide nutrient broth medium (beef extract 0.3g, peptone 1g, sodium chloride 0.5g, add water to 100ml volume, pH7.4-7.6) In 250mL containers, 50mL in each container, after sealing and bandaging, sterilize at 121°C for 20min; after cooling, insert the bacteria loop cultured on a slant for 24h, and shake the flask at a speed of 200r / min and a temperature...

Embodiment 3

[0065] The flax degumming process is as follows:

[0066]Put 5g of the sterilized flax stem into a 250ml conical flask, divide the flax flax and the fermented bacteria liquid into the conical flask at a bath ratio of 1:20, and fill the liquid with a volume of 100ml. Respectively degumming for 1 day, 2 days and 4 days; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5 minutes, rinse with tap water several times to remove bacteria on the flax, and stop degumming.

[0067] Through the observation of degummed flax, it was found that the flax fibers were obviously dispersed after 1 day, and the color of the fermentation broth became dark and turbid; after 2 days of degumming, the original bundled flax was completely dispersed into fibers, and the fermentation broth was obviously thickened; after 4 days of degumming, the degumming rate of flax reached more than 90 percent.

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Abstract

The invention relates to a fluorescent pseudomonad DA4 strain as well as an acquisition method and application thereof. The fluorescent pseudomonad DA4 strain has pectase and hemicellulase producing capacity and flax degumming activity. The fluorescent pseudomonad strain screened by the invention has fast propagation, high antipollution capacity and good heat and alkali resistance; since a composite degumming enzyme solution is used for flax degumming, the degumming period is shortened greatly, and the system and the culturing process is safe for operation without toxicity or environmental pollution; the invention can shorten the flax degumming period, enhance the flax yield and the strength of flax fibers, improve the quality of the flax fibers by adopting the composite degumming enzyme solution for flax microbial degumming, thereby being beneficial to popularization; in addition, the invention can not only be used for flax raw stem degumming, roving scouring and the biological pretreatment of flax fabrics, but also can be used for the degumming and the biological pretreatment of hemp bast fibers, i.e. ramie, jute, hemps, and the like.

Description

technical field [0001] The invention belongs to the field of Pseudomonas fluorescens strains and applications thereof, in particular to a DA4 strain of Pseudomonas fluorescens and its obtaining method and application. Background technique [0002] At present, the traditional flax warm water retting, rain dew retting and natural water retting methods are commonly used in the production of flax industry, all of which use natural microorganisms and their enzymes to degrade the pectin, hemicellulose and lignin of flax , the strains are mixed, it is difficult to effectively control, resulting in long degumming time (warm water retting is about 4d; rain dew retting is 15-20d; natural water retting is 5-7d), the comprehensive growth rate is low, and the quality is unstable (bundle Many problems such as low fiber strength) and heavy environmental pollution have seriously restricted the vigorous development of the flax industry. It is worth noting that the deficiencies in flax degum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20D01C1/04D06M16/00C12S11/00C12R1/39D06M101/06
Inventor 丁若垚张兴群郁崇文董政娥
Owner DONGHUA UNIV
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