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A Prism-Coupled Surface Plasmon Resonance Biosensor

A biosensor and coupled technology, applied in the field of biosensors, achieves the effects of simplifying the construction and operation process, low cost, and facilitating popularization and application

Inactive Publication Date: 2011-11-30
BEIJING GP MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The invention provides a new SPR biosensor, which is a prism-coupled SPR biosensor that directly deposits a multi-metal film layer on the surface of an optical plastic prism, and solves the problems existing in the existing SPR biosensor

Method used

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  • A Prism-Coupled Surface Plasmon Resonance Biosensor
  • A Prism-Coupled Surface Plasmon Resonance Biosensor
  • A Prism-Coupled Surface Plasmon Resonance Biosensor

Examples

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Effect test

Embodiment 1

[0072] Embodiment 1 A kind of prism-coupled SPR biosensor

[0073] In this embodiment, the material of the optical plastic prism 10 is selected from polycarbonate with a refractive index of 1.586, which is processed into a semicircular prism by injection molding. Due to the high refractive index of polycarbonate, substances with large refractive indices can be measured. The water absorption rate of polycarbonate optical plastic is low, soaked in water for 24 hours, the weight gain is only 0.13%. In addition, it not only has good chemical resistance and heat resistance, but also has high impact strength, good ductility, and uniform molding shrinkage, which is convenient for injection molding.

[0074] In the present embodiment, the inner metal film layer 12 is a silver film, which is deposited on the optical plastic prism 10 by vacuum coating, and its thickness is 23nm; because the surface energy of plastics is generally relatively low and the surface polarity is poor, in orde...

Embodiment 2

[0077] Embodiment 2 A prism-coupled SPR biosensor

[0078]In this embodiment, the material of the optical plastic prism 10 is selected from cycloolefin copolymers with a refractive index of 1.535. It is processed into a semicircular cross-sectional prism by injection molding. The cycloolefin copolymer has good thermal stability and moisture resistance, and the water absorption (24h) is less than 0.01%. It is chemically stable and can reproduce fine surface contours.

[0079] In the present embodiment, the inner metal film layer 12 is a silver film, which is deposited on the optical plastic prism 10 by vacuum coating, and its thickness is 42nm; because the surface energy of plastics is generally relatively low and the surface polarity is poor, in order to prevent the inner metal film The layer 12 falls off from the surface of the optical plastic prism 10, and there is also a bonding metal film layer 11 between the optical plastic prism 10 and the inner metal film layer 12, and...

Embodiment 3

[0082] Example 3 Application of a prism-coupled SPR biosensor in the detection of human papillomavirus

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Abstract

The invention relates to a prism-coupled SPR biosensor, which comprises an optical plastic prism, an inner metal film layer, an outer metal film layer and a biological component layer; The outer metal film layer is deposited on the film layer, the inner metal film layer and the outer metal film layer can be two kinds of the same or different metal film layers, and the sum of the thickness of the inner metal film layer and the outer metal film layer is between 20-80nm The space meets the optical requirements for exciting surface plasmon resonance, and a biological component layer is fixed on the surface of the outer metal film layer. The present invention also relates to the use of the prism-coupled SPR biosensor in preparing biochips and its use in detecting interactions between biological samples.

Description

technical field [0001] The invention relates to a biosensor, in particular to a prism-coupled surface plasmon resonance biosensor. Background technique [0002] A sensor is a device that acquires and processes information. The device for sensing material composition is a chemical sensor, which is a miniaturized device that can specifically and reversibly respond to a certain chemical composition, and can generate a measurable signal proportional to the concentration of the composition. Biosensors are a special kind of chemical sensors, which use biologically active units (such as antibodies, nucleic acids, proteins, etc.) as biological sensitive units, and are highly selective detectors for the measured target. It uses various physical and chemical signal converters to capture the reaction between the target object and the sensitive element, and then express the degree of reaction with discrete or continuous electrical signals, so as to obtain information such as the concen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/41C12Q1/70C12Q1/68
Inventor 刘建军裴晓飞杨华卫陈忠吴晓东
Owner BEIJING GP MEDICAL TECH
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