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CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof

A sensing method and biosensor technology, applied in the field of gold nanometer SPR sensor and its preparation, can solve problems such as false positives, and achieve the effects of convenient operation, low detection cost, and increased SPR angle

Active Publication Date: 2020-01-03
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is how to improve the sensitivity and specificity of detecting CTC, and how to improve the false positive problem of specific recognition by a single antigen antibody in the process of detecting CTC

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  • CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof
  • CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof
  • CTC detection SPR sensor having high sensitivity and dual selectivity and preparation method thereof

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Embodiment Construction

[0034] The following describes several preferred embodiments of the present invention with reference to the accompanying drawings, so as to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned herein.

[0035] In the present invention, the gold nanoparticles wrapped in the cell membrane can be replaced with other nanoparticles, including magnetic nanoparticles and nanometer quantum dots, so as to achieve different detection purposes.

[0036] The principle of the present invention is to simulate artificial CTC samples by overexpressing JUP and adding human breast cancer cell line MCF-7. Prolinker is a calixarene derivative containing -SH. Prolinker can bind to the chip surface through gold-sulfhydryl bonds. The calixarene cavity of Prolinker can be used to anchor JUP antibodies to the chip surface. ...

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Abstract

The invention discloses an SPR biosensing system for detecting a CTC. The SPR biosensing system includes an SPR biosensor, a cell membrane functionalized nanoparticle and a folate modified nanoparticle; the SPR biosensor includes an immunochip having a surface modified by a JUP antibody; the cell membrane functionalized nanoparticle contains a JUP protein and a folate receptor; the cell membrane functionalized nanoparticle is combined with the immunochip via an interaction of an antigen-antibody; and the folate modified nanoparticle is combined with the cell membrane functionalized nanoparticle via an interaction of a folate-folate receptor. According to the SPR biosensing system provided by the invention, the CTC is combined at dual selectivity by using the cell membrane functionalized nanoparticle and the folate modified nanoparticle, a false positive problem caused by single antigen-antibody specificity identification is improved, the detection cost is low, and the operation is convenient.

Description

technical field [0001] The invention relates to the field of detection of circulating tumor cells, in particular to an SPR sensor based on cell membrane functionalized gold nanometers and a preparation method thereof. Background technique [0002] Circulating tumor cells (CTCs), a class of cells released from neoplastic lesions into the blood system, play a key role in the spread of metastatic cancer. CTCs can reflect the risk of tumor metastatic progression and can facilitate precision therapy by detecting CTCs in blood. Therefore, the detection of CTCs is very important for cancer. Currently, CTCs are mainly enriched and detected based on their biological or physical properties, such as specific proteins on the cell surface, cell size, and charged properties of the cells. Commonly used CTC detection methods include immunomagnetic bead method and microfluidic technology. In addition, CellSearch, invented by Veridex, is the only commercial product approved by FDA and CFDA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 陈红霞
Owner SHANGHAI UNIV
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