Method for virus detection by surface plasma resonance technology and chip used in the same

Abstract

The invention provides a method for detecting viruses through a surface plasma resonance technology (SPR), which comprises the steps as follows: a sample to be detected is mixed with a buffer solution including an SSPE solution and a dextran sulfate solution, and the formed mixture contains a solution of 1-10 multiplied by SSPE and 0.5 to 10 percent of the dextran sulfate solution; the mixture isled to flow through an SPR biosensor chip; a probe special to the viruses is connected with the chip; the signal difference of corresponding flowing pools before and after the mixture flows through the chip is measured through the SPR; and the signal difference and the signal difference of a comparison flowing pool are compared so as to determine whether the viruses exist. The method is especially suitable for detecting human papillomavirus (HPV). In addition, the invention further provides the SPR biosensor chip used in the method.

Description

field of invention [0001] The invention belongs to the field of virus detection. Specifically, it relates to a virus detection method using surface plasmon resonance technology (Surface Plasmon Resonance, SPR), and the method is particularly useful for detecting human papillomavirus (Human Papillomavirus, HPV). The present invention also relates to an improved SPR biosensor chip used in the above method, and a kit comprising the chip and nucleic acid probes. Background technique [0002] Surface Plasmon Resonance (SPR) is a technology that uses the physical optics phenomenon caused by the total reflection of light at the metal film / liquid interface to analyze the interaction between molecules. The SPR instrument consists of an automatic sampling manipulator, a high-resolution CCD camera, and a gold-plated or silver-plated SPR biosensor chip with one or more flow cells on it. SPR technology has been widely used in the detection and analysis of the interaction between biomol...

AI Technical Summary

Problems solved by technology

[0014] (3) Real-time fluorescent quantitative PCR (real-time quantitative PCR, RQ-PCR): For the typing detection of HPV, the general real-time fluorescent quantitative PCR uses type-specific primers, which are equivalent to specific PCR, only with quantitative functions, However, for HPV typing, the workload of type-specific real-time PCR is too large, and the effectiveness of scorpion primers and probes for real-time PCR based on molecular probes needs a lot of verification and improvement, and the current real-time fluorescent PCR The cost of the experiment is relatively high, which also limits its wide application

However, the cost is high, and the reliability and practicability of the detection have yet to be verified.

Images