Cytomegalovirus antibody aptamer specific to cytomegalovirus, and biochip formed by same

Abstract

The invention discloses a cytomegalovirus antibody aptamer specific to cytomegalovirus, and a biochip formed by the cytomegalovirus antibody aptamer simultaneously. Compared with the antibody in the traditional detection technique, the oligonucleotide aptamer disclosed by the invention has the advantages of stronger binding force with a target molecule, good stability, high accuracy, good repeatability, shorter screening period and the like, and can be repeatedly used, stored for a long term and used for accurate site modification. When put into an SPR (surface plasmon resonance) biosensor for detection, the obtained biochip can realize real-time detection, no need of marking samples, a very small required amount of samples, convenient, quick and highly-sensitive detection process, wide range of application, high flux, and detection of a turbid or even opaque sample under most conditions.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a giant cell antibody aptamer specific to cytomegalovirus and a biological chip capable of detecting whether the giant cell is infected in the early stage of pregnant women. Background technique [0002] Birth defects bring great mental and economic burdens to families, countries and society. At present, there is no effective treatment for fetal congenital defects. Among the methods of preventing birth defects, the primary prevention in the first trimester is the most effective. Giant cells are a group of pathogenic microorganisms infected during pregnancy, which can cause intrauterine infection through the placenta or birth canal, leading to miscarriage, stillbirth or fetal growth retardation, congenital malformations, neonatal infection, and growth and development disorders of infants and young children. About 26,000 children with cytomegalovirus infection are born in my...

AI Technical Summary

Problems solved by technology

However, according to statistical data, ELISA and gold standard methods are commonly used in clinical diagnosis in my country to detect serum levels of TOX-IgG and CMV-IgG in pregnant women, and the false positive rate is high, which cannot provide accurate diagnostic basis for clinical use.

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