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Method for quickly determining ethyl carbamate (EC) in alcoholic drink

A medium urethane, rapid determination technology, applied in the field of analysis and detection, can solve problems such as interference and limited applicability

Inactive Publication Date: 2012-05-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the CW / DVB head has been discontinued. Although the extraction efficiency of the DVB / CAR / PDMS three-phase head is higher than that of the PA head, the interference is serious when it is used for wines with complex wine components such as yellow rice wine. Quantification brings great difficulty, which limits the applicability of this method
At present, there is no literature or patent that discloses the use of PA extraction head and corresponding extraction and separation conditions for the determination of EC in drinking wine

Method used

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  • Method for quickly determining ethyl carbamate (EC) in alcoholic drink
  • Method for quickly determining ethyl carbamate (EC) in alcoholic drink
  • Method for quickly determining ethyl carbamate (EC) in alcoholic drink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Rapid Determination of EC in Yellow Rice Wine

[0016] The present invention selects a commercially available rice wine, dilutes the wine sample to a final alcohol content of 4% to 20% (v / v) with redistilled deionized water, draws 8 mL of the diluted wine sample and places it in a 20 mL headspace bottle , add 8μL PC internal standard solution (150mg / L), then add 3g sodium chloride, tighten the bottle cap, insert 85μm PA extraction head for headspace solid-phase microextraction and gas chromatography-mass spectrometry to detect EC content. The measured EC content is shown in Table 1.

[0017] Table 1 EC content in a commercial rice wine

[0018] wine sample EC content (μg / L) A commercially available rice wine 87.14

Embodiment 2

[0019] The rapid determination of EC in the embodiment 2 wine

[0020] The present invention selects a commercially available wine, dilutes the wine sample with redistilled deionized water to a final alcohol content of 4% to 20% (v / v), draws 8 mL of the diluted wine sample and places it in a 20 mL headspace bottle , add 8μL PC internal standard solution (150mg / L), then add 3g sodium chloride, tighten the bottle cap, insert 85μm PA extraction head for headspace solid-phase microextraction and gas chromatography-mass spectrometry to detect EC content. The measured EC content is shown in Table 2.

[0021] Table 2 EC content in a commercially available wine

[0022] wine sample EC content (μg / L) a commercial wine 10.37

Embodiment 3

[0023] Example 3 Rapid Determination of EC in Dew Wine

[0024] The present invention selects a commercially available wine, dilutes the wine sample with redistilled deionized water to a final alcohol content of 4% to 20% (v / v), draws 8 mL of the diluted wine sample and places it in a 20 mL headspace bottle , add 8μL PC internal standard solution (150mg / L), then add 3g sodium chloride, tighten the bottle cap, insert 85μm PA extraction head for headspace solid-phase microextraction and gas chromatography-mass spectrometry to detect EC content. The measured EC content is shown in Table 3.

[0025] Table 3-EC content in commercially available wine

[0026] wine sample EC content (μg / L) A commercially available liquor 25.11

[0027] Beverage wine applicable to the present invention is not limited to yellow rice wine, wine, dew wine, but also includes fruit wine, mixed wine, cooking wine and the like.

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PUM

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Abstract

The invention discloses a method for quickly determining ethyl carbamate (EC) in alcoholic drink, which comprises the following steps of: diluting an alcoholic drink sample to be tested into the alcoholic drink sample with final alcoholic content of 4 percent to 20 percent (v / v) by double distilled deionized water; concentrating EC in the alcoholic drink sample through a head space solid-phase micro-extraction technology without a complex preprocessing process or organic solvent; carrying out detection and quantification analysis through a gas chromatography-mass spectrometer technology; and comparing with a standard curve to calculate the content of EC in the alcoholic drink sample. The method disclosed by the invention can be used for quickly testing and determining the EC in the alcoholic drink, the detection limit can reach as low as 1.19 mug / L, the linear correlation coefficient is larger than 0.99, the recovery rate is 90.21 percent to 99.07 percent, and the relative standard deviation is less than 10 percent. The method overcomes defects of the prior determining method, and can be used for detection and quantification analysis of the EC in the alcoholic drink.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, in particular to a rapid detection method for ethyl carbamate in drinking wine. Specifically, it relates to a method for detecting ethyl carbamate in beverage wine by applying headspace solid-phase microextraction and gas chromatography-mass spectrometry. Background technique [0002] The content of ethyl carbamate (EC for short) in drinking alcohol is very small, mostly in the range of several μg / L to hundreds of μg / L. In the traditional determination, the pretreatment methods of solvent extraction (LLE) and solid phase extraction (SPE) are cumbersome, time-consuming and labor-intensive, and require the use of certain hazardous organic solvents, which is not conducive to the health of analysts, nor is it economical and environmentally friendly. [0003] Headspace-solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) is used to measure EC in beverage win...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 徐岩刘俊赵光鳌
Owner JIANGNAN UNIV
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