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Novel method for rapid detection of aflatoxin in food

A kind of technology of aflatoxin and new method, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult separation, poor purification effect, unclear materials used, etc., and achieve simple separation operation, good purification effect and small dosage Effect

Active Publication Date: 2016-08-31
云南伦扬科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the invention patent CN104914196A has also been improved, using HC-C18+PSA as the purification material, but the material used is not clear, and there are problems of separation difficulty and poor purification effect

Method used

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  • Novel method for rapid detection of aflatoxin in food
  • Novel method for rapid detection of aflatoxin in food
  • Novel method for rapid detection of aflatoxin in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 utilizes the present invention to detect the content of aflatoxin in peanut, and the steps are:

[0031] 1. Preparation of organic acid-coated magnetic nanomaterials: Weigh 4.10 g ferrous ammonium sulfate ((NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O), 13.52g ferric chloride (FeCl 3 ·6H 2 0), after dissolving with 100mL deionized water, transfer to a 250mL three-necked bottle, under nitrogen protection, heat to 80°C in a water bath, mechanically stir for 10min (rotating speed is 1000rpm), then add 5mL methanol containing 200μL n-nonanoic acid solution, continue to stir for 5 minutes, add 10 mL of concentrated ammonia water, continue to react the mixed solution for 30 minutes, cool to room temperature naturally, collect the product with an external magnetic field and discard the reaction solution, and wash the magnetic material twice with 100 mL of deionized water and twice with 100 mL of methanol , washed three times with 100 mL deionized water, and dried in vacuum ...

Embodiment 2

[0040] Embodiment 2 Utilizes the present invention to detect the content of aflatoxin in corn, and the steps are:

[0041] 1. Preparation of organic acid-coated magnetic nanomaterials: Weigh 2.00 g of ferrous chloride (FeCl 2 4H 2 O), 5.40g ferric chloride (FeCl 3 ·6H 2 O), after dissolving with 100mL deionized water, transfer to a 250mL three-necked bottle, under nitrogen protection, mechanically stir for 15min (rotational speed is 600rpm) and water bath heating to 70°C, then add 5mL methanol containing 300μL n-decanoic acid solution, continue to stir for 5 minutes, add 15 mL of concentrated ammonia water, continue to react the mixture for 30 minutes, cool to room temperature naturally, collect the product with an external magnetic field and discard the reaction solution, and wash the magnetic material twice with 100 mL of deionized water and twice with 100 mL of methanol , washed twice with 100 mL of deionized water, and vacuum-dried at 80°C for 24 hours to obtain n-decan...

Embodiment 3

[0045] Embodiment 3 utilizes the present invention to detect the content of aflatoxin in the walnut, and the steps are:

[0046] 1. Preparation of organic acid-coated magnetic nanomaterials: Weigh 2.00 g of ferrous chloride (FeCl 2 4H 2 O), 5.62g iron sulfate (Fe 2 (SO 4 ) 3 9H2O), dissolved in 100mL deionized water, transferred to a 250mL three-necked bottle, under nitrogen protection, mechanically stirred for 12min (rotation speed: 800rpm) and heated to 85°C in a water bath, then added 5mL acetone containing 400μL oleic acid solution, continue to stir for 5 minutes, add 20 mL of concentrated ammonia water, continue to react the mixed solution for 30 minutes, cool to room temperature naturally, collect the product with an external magnetic field and discard the reaction solution, wash the magnetic material twice with 100 mL of deionized water, and wash three times with 100 mL of methanol , washed twice with 100 mL of deionized water, and dried in vacuum at 70° C. for 30 h...

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PUM

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Abstract

The invention discloses a novel method for rapid detection of aflatoxin in food. The novel method studies extraction, purification and enrichment of aspertoxin in food and detects aflatoxin in food by using a combination of high performance liquid chromatography, on-line post-column photochemical derivatization and a fluorescence detector. The method employs a combination of dispersive magnetic solid-phase micro-extraction and dispersive liquid-liquid micro-extraction techniques for extraction, purification and enrichment of aspertoxin in a sample. Compared with a conventional determination method for aflatoxin, the method provided by the invention has the characteristics of a small amount of an organic solvent used in detection, short analysis time, simple operation, low detection cost, accuracy, etc.

Description

technical field [0001] The invention relates to a new method for rapidly detecting aflatoxin in food. It belongs to the field of food testing. Background technique [0002] Aflatoxins (AFT) are a class of compounds with similar chemical structures, all of which are derivatives of dihydrofuranocoumarins. Aflatoxin (AFT) is a highly toxic fungal metabolite discovered in the early 1960s. It is mainly produced by Aspergillus flaws and Aspergillus parasiticus after infecting agricultural products. It is one of the strongest known carcinogens and is extremely toxic. In 1993, aflatoxin was classified as a first-class carcinogen by the World Health Organization Cancer Research Agency. [0003] With the frequent occurrence of aflatoxin pollution incidents in the world and in my country, the monitoring of the food field in various countries is becoming more and more strict. At present, the commonly used detection methods include enzyme-linked immunosorbent assay (ELASA), immunoaff...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/027G01N2030/062G01N2030/143
Inventor 焦扬杨亚玲赵娇
Owner 云南伦扬科技有限公司
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