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Yeast surface atom transfer imprinted adsorbent, and preparation method and application thereof

A technology of imprinted adsorption and atom transfer, which is applied in chemical instruments and methods, adsorption water/sewage treatment, and other chemical processes, can solve problems that have not been reported, and achieve the effect of cheap price, good adsorption performance, and high yield

Inactive Publication Date: 2012-06-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to reduce the cost of synthetic materials and improve the compatibility of materials, biological materials are ideal imprinting matrix materials. As a kind of cheap, easy to obtain and safe industrial microorganisms, the abundant groups on their surface can improve the compatibility with polymers. Stability, recently, our atom transfer initiator is loaded on the surface of yeast, and then the imprinting polymerization process is carried out on its surface. At present, the research on obtaining imprinted polymeric adsorbents by imprinting on the surface of yeast by atom transfer radical polymerization has not been reported.

Method used

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  • Yeast surface atom transfer imprinted adsorbent, and preparation method and application thereof
  • Yeast surface atom transfer imprinted adsorbent, and preparation method and application thereof
  • Yeast surface atom transfer imprinted adsorbent, and preparation method and application thereof

Examples

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Embodiment 1

[0025] (1) Preparation of yeast surface-loaded initiator

[0026] Add yeast to a mixture of thionyl chloride and benzene at a volume ratio of 50:10 (mL), wherein yeast and benzene are added at a mass-to-volume ratio of 1 g:10 mL, and react at 70°C After 24 h, wash three times with tetrahydrofuran, dry in vacuum at 25 ℃, take the dried product and disperse it in a mixed solution of tetrahydrofuran and anhydrous triethylamine with a volume ratio of 30:1 (mL). Water triethylamine is 1 g: 1 mL according to the mass and volume ratio, and isobutyryl bromide with a volume ratio of 1:1 (mL) to anhydrous triethylamine is added dropwise after passing nitrogen gas in an ice bath to evacuate oxygen. React at room temperature for 12 h, wash with ethanol three times, and vacuum-dry at 25 °C to obtain the yeast initiator.

[0027] (2) Preparation of yeast surface imprinted adsorbents (MIPs)

[0028]Add the template molecule cephalexin to the mixed solution of methacrylic acid and ethylene...

Embodiment 2

[0032] (1) Preparation of yeast surface-loaded initiator

[0033] Add yeast to a mixture of thionyl chloride and benzene at a volume ratio of 100:10 (mL), wherein yeast and benzene are added at a mass-to-volume ratio of 1.5 g:10 mL, and react at 80 °C 28 h, washed three times with tetrahydrofuran, dried under vacuum at 35 °C, and dispersed the dried product in a mixed solution of tetrahydrofuran and anhydrous triethylamine with a volume ratio of 40:1 (mL), wherein the dried product was mixed with no The water triethylamine is 1.5 g: 1 mL according to the mass and volume ratio, after passing nitrogen and evacuating oxygen in an ice bath ， Add isobutyryl bromide with a volume ratio of 2:1 (mL) to anhydrous triethylamine dropwise, react at room temperature for 16 h, wash with ethanol three times, and vacuum-dry at 40 °C to obtain the yeast initiator.

[0034] (2) Preparation of yeast surface imprinted adsorbents (MIPs)

[0035] Add the template molecule cephalexin to the mixed...

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Abstract

The invention relates to the technical field of preparation of an environmental material, in particular to a yeast surface atom transfer imprinted adsorbent, and a preparation method and application thereof. The preparation method comprises the following steps of: through the atom transfer radical polymerization process, firstly, loading an initiator into a yeast surface to obtain a matrix material with the initiator; and then preparing the yeast surface imprinted adsorbent by using cefalexin as template molecules, using methacrylic acid (MAA) as a functional monomer, using ethylene glycol dimethacrylate (EGDMA) as a cross linking agent and using CuCl as a catalyst. The spherical imprinted adsorbent has obvious thermal and magnetic stability. The research on the effect between the template molecules and the functional monomer by ultraviolet rays shows that the acting force exists. A static adsorption experiment is used for researching the adsorption equilibrium, the dynamics and the selective recognition performance of the prepared imprinted adsorbent. A result shows that the yeast surface imprinted adsorbent obtained by utilizing the preparation method disclosed by the invention has high adsorption capacity, high-speed adsorption dynamics property and obvious cefalexin molecular recognition performance.

Description

technical field [0001] The invention relates to the technical field of environmental material preparation, in particular to a yeast surface atom transfer imprinting adsorbent and its preparation method and application. Background technique [0002] Molecular imprinting technology uses the target analyte as a template molecule, combines structurally complementary functionalized polymer monomers with the template molecule through covalent or non-covalent bonds, and adds a cross-linking agent for polymerization reaction. After the reaction is completed The template molecules are eluted to form a molecularly imprinted polymer (MIPs) technology with a fixed hole size and shape and a definite arrangement of functional groups. The surface imprinting technology establishes molecular recognition sites on the surface of the matrix material. , which better solve some serious defects in the traditional molecular imprinting technology as a whole, such as too deep embedding of active site...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/06C08F2/44C08J9/28B01J20/26B01J20/28B01J20/30C02F1/28C02F1/58
Inventor 李秀秀潘建明戴江栋孟敏佳徐龙城李春香闫永胜
Owner JIANGSU UNIV
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