Method for preparing hyperin from Dogbane leaves
A technology of hypericin and ethanol, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of difficult extraction and separation, complex chemical components, low purity of flavonoids, etc., and achieves high extraction efficiency, The effect of strong operability and high extraction and separation efficiency
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Embodiment 1
[0031] The preparation method of hyperin in apocynum leaf specifically comprises the following steps:
[0032] (1) Pick, wash and dry the Apocynum leaf medicinal material, put it into an extraction tank, add 10 times the amount of methanol, heat and reflux at 85°C for 2 times, filter for 2 hours each time, combine the extracts, concentrate, and obtain Apocynum apocynum Hemp leaf extract, set aside;
[0033] (2) get the Apocynum apocynum leaf extract that step (1) obtains and add ethanol to make the concentration of ethanol reach 80%, leave standstill, precipitate, filter out solution, concentrate, obtain the alcohol precipitation solution of Apocynum apocynum leaf extract and stand-by;
[0034] (3) Get the D101 type macroporous resin column on the Apocynum leaf alcohol precipitation liquid that step (2) obtains, first wash 5 ~ 10 column volumes with water, then use 10% ethanol to elute 1 ~ 3 column volumes, and then use 60% ethanol was eluted for 3 to 5 column volumes, detect...
Embodiment 2
[0037] The preparation method of hyperin in apocynum leaf specifically comprises the following steps:
[0038] (1) Pick, wash, and dry the Apocynum leaf medicinal material and put it into an extraction tank, add 8 times the amount of 80% ethanol, heat and reflux at 85°C for 3 times, filter for 1.5 hours each time, combine the extracts, concentrate, Apocynum leaf extract is obtained, for subsequent use;
[0039] (2) get the Apocynum apocynum leaf extract that step (1) obtains and add ethanol to make the concentration of ethanol reach 85%, leave standstill, precipitate, filter out solution, concentrate, obtain the alcohol precipitation solution of Apocynum apocynum leaf extract and stand-by;
[0040] (3) AB-8 type macroporous resin column on AB-8 type macroporous resin column on the Apocynum leaf alcohol precipitation liquid that step (2) obtains, first wash 5~10 column volumes with water, then use 20% ethanol to elute 1~3 column volumes, Then eluted with 55% ethanol for 3~5 co...
Embodiment 3
[0043] The preparation method of hyperin in apocynum leaf specifically comprises the following steps:
[0044] (1) Pick, wash, and dry the Apocynum leaf medicinal material and put it into an extraction tank, add 10 times the amount of 50% ethanol, heat and reflux at 85°C for 2 times, filter for 3 hours each time, combine the extracts, concentrate, Apocynum leaf extract is obtained, for subsequent use;
[0045] (2) get the Apocynum apocynum leaf extract that step (1) obtains and add ethanol to make the concentration of ethanol reach 75%, leave standstill, precipitate, filter out solution, concentrate, obtain the alcohol precipitation solution of Apocynum apocynum leaf extract and stand-by;
[0046] (3) Get the HPD100 type macroporous resin column on the Apocynum leaf alcohol precipitation liquid that step (2) obtains, first wash 5 ~ 10 column volumes with water, then use 10% ethanol to elute 1 ~ 3 column volumes, and then use Elute with 50-70% ethanol for 3-5 column volumes, d...
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