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Method for extracting flavonoid glycoside compounds in herba epimedii

A technology of flavonoid glycosides and flavonoid glycosides in Huozhong, which is applied in the field of traditional Chinese medicine extraction, can solve the problems of flammability and explosion, and achieve the effect of simple operation, mild extraction conditions and low loss

Active Publication Date: 2018-08-03
SHENYANG PHARMA UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to address the problems existing in the prior art, and to provide a method for utilizing a deep eutectic solvent to extract flavonoid glycosides in Epimedium. Using the present invention to extract flavonoid glycosides can effectively improve the yield of flavonoid glycosides. Improve the extraction efficiency and solve the problems existing in the use of flammable and explosive organic solvents in the existing extraction technology of flavonoid glycosides

Method used

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  • Method for extracting flavonoid glycoside compounds in herba epimedii
  • Method for extracting flavonoid glycoside compounds in herba epimedii
  • Method for extracting flavonoid glycoside compounds in herba epimedii

Examples

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Embodiment 1

[0038] Prepare the deep eutectic solvent of choline chloride and phenol:

[0039] Choline chloride and phenol with a molar ratio of 1:2 were stirred under anhydrous conditions, heated at 60°C until a uniform and stable transparent solution was formed, and sealed for storage.

[0040] In the invention, the deep eutectic solvent is used for the treatment of flavonoid glycoside standard products, and the flavonoid glycoside substances are sensitively detected by means of high-performance liquid chromatography. Specific steps are as follows:

[0041] 1) Weigh a certain amount of labeled products of four flavonoid glycosides Epimedin A, Epimedin B, Epimedin C, and Icariin, and add methanol to form Epimedin A, Epimedin B, Epimedin B, and Epimedin B. A mixed solution of flavonoid glycosides with epimedin C and icariin concentrations of 0.040, 0.039, 0.171, and 0.273 mg / ml, respectively;

[0042] 2) Measure 2.0ml of the mixed solution obtained in step (1), add 4.0ml of deep eutectic...

Embodiment 2

[0049] A method utilizing a deep eutectic solvent to extract flavonoid glycosides in Epimedium leaves, comprising the following steps:

[0050] 1) According to the method described in Example 1, a deep eutectic solvent of choline chloride-phenol (1:2) was prepared.

[0051] 2) Grind dried Epimedium, pass through a 80-mesh sieve, take 0.05 grams of Epimedium powder, add 2ml of choline-like deep eutectic solvent, extract by ultrasonic at 40°C for 30 minutes, power 100W / cm 2 , add 2 ml of water to dilute, and vortex for 2 minutes to obtain flavonoid glycosides extract;

[0052] 3) Add water to the flavonoid glycoside compound extract obtained in step 2) to make up to 10 ml, filter through a 0.45 μm filter membrane to remove impurities, and obtain a filtered extract.

[0053] Inject 5 μL of the obtained extract into a high-performance liquid chromatograph for detection, and use the detection conditions under the item of Example 1 for detection. Chromatogram see figure 2. The ...

Embodiment 3

[0055] A method utilizing a deep eutectic solvent to extract flavonoid glycosides in Epimedium leaves, comprising the following steps:

[0056] 1) According to the method described in Example 1, a deep eutectic solvent of choline chloride-1,2-propanediol (1:2) was prepared.

[0057] 2) Take 0.05 g of Epimedium powder that has been crushed and passed through a 40-mesh sieve, add 2 ml of a choline-based deep eutectic solvent, and conduct ultrasonic extraction at 50°C for 40 minutes with a power of 100W / cm 2 , add 2 ml of water to dilute, and vortex for 2 minutes to obtain flavonoid glycosides extract;

[0058] 3) Add water to the flavonoid glycoside compound extract obtained in step 2) to make up to 10 ml, filter through a 0.45 μm filter membrane to remove impurities, and obtain a filtered extract.

[0059] The obtained extract was detected by injecting 5 μL of samples into a high-performance liquid chromatograph, and the yields of epimedin A, epimedin B, epimedin C and icariin...

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Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, and relates to a method for extracting flavonoid glycoside compounds in herba epimedii, in particular to a method for extracting flavonoid glycoside compounds in epimedium leaves with a deep eutectic solvent. According to the preparation method, the flavonoid glycoside compounds are extracted from the raw material of herba epimedii with the deep eutectic solvent, particularly a choline deep eutectic solvent, as an extracting agent and water as a diluent for reducing the system viscosity under ultrasonic enhancement. The extraction method has higher extraction efficiency for flavonoid glycoside components, is more thorough in extraction and has the extraction efficiency close to 100%. The method is mildin extraction condition and convenient to operate, has the characteristics of no blast or volatilization of the extracting agent, low loss, low energy consumption and the like and can be applied in efficient and green large-scale production of the flavonoid glycoside compounds and comprehensive utilization of the herba epimedii resource.

Description

【Technical field】 [0001] The invention relates to the technical field of traditional Chinese medicine extraction, and relates to a method for extracting flavonoid glycosides in Epimedium, in particular to a method for extracting flavonoid glycosides in Epimedium leaves by using a deep eutectic solvent. 【Background technique】 [0002] Deep eutectic solvent is a new type of solvent, which is a solvent that combines hydrogen bond donors and hydrogen bond acceptors to destroy the crystal lattice and greatly reduce the melting point. It has been a research hotspot in the field of chemical industry for several years. Due to the advantages of simple synthesis, low synthesis cost, and green performance, deep eutectic solvents have been applied in the fields of electrochemistry, materials, energy, and organic synthesis. Compared with traditional organic solvents, deep eutectic solvents have the characteristics of low melting point, low cost, low vapor pressure, degradable and renewa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/296
CPCA61K36/296A61K2236/33A61K2236/35A61K2236/53
Inventor 赵龙山王洋赵晶李坤杰
Owner SHENYANG PHARMA UNIVERSITY
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