Water-soluble Chitooligosaccharide preparation method

A chitooligosaccharide and water-soluble technology is applied in the field of preparation of water-soluble chito-oligosaccharide, which can solve problems such as complicated processes, and achieve the effects of simple process and good tumor-inhibiting activity.

Inactive Publication Date: 2004-11-10
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, water-soluble chitooligosaccharides with a high degree of polymerization are prepared by deacetylating chitin first, then degrading chitosan to a certain degree of polymerization, and then partially acetylating it with acetic anhydride. The process is relatively cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Take 100 grams of crab shell chitosan with a degree of deacetylation of 80.2%, dissolve it with 2 liters of 2% acetic acid solution, adjust the pH of the solution to 5.5 with saturated sodium hydroxide, control the temperature at 47°C, and add 10 milliliters of hemicellulose Suzyme crude enzyme solution catalyzes the degradation of chitosan. When the reaction solution is dripped into 2% sodium hydroxide solution without turbidity, use NMWL 10kDa ultrafiltration membrane to remove enzymes, and the solution that passes through the 10kDa ultrafiltration membrane is further fractionated with 3kDa and 1kDa ultrafiltration membranes to obtain solutions of three components . CHU10-3 is the fraction that passed through 10kDa membrane but failed to pass through 3kDa membrane; CHU3-1 is the fraction that passed through 3kDa membrane but failed to pass through 1kDa membrane. After the filtrate was concentrated in vacuo, the pH solution was adjusted to 8-9 with NaOH, se...

Embodiment 2

[0016] Example 2: Add 50 grams of shrimp shell chitosan with a deacetylation degree of 71.2% into 2 liters of 2% acetic acid solution and stir for 8 hours, then filter, adjust the pH of the solution to 5.0, control the temperature at 40°C, and add lysozyme to catalyze the degradation of chitosan sugar. After 24 hours, microfiltration was performed first, and then the oligosaccharide fraction was separated by ultrafiltration with a NMWL 1kDa ultrafiltration membrane. The component solution that did not pass was adjusted to pH 8-9 with saturated sodium hydroxide, centrifuged, and the supernatant was concentrated in vacuum, then ethanol sedimented, washed with ethanol, and dried to obtain a water-soluble chitooligosaccharide product with a yield of 38.7%. The average degree of polymerization is 21, and the degree of dispersion is 2.65.

Embodiment 3

[0017] Example 3: Take 25 grams of shrimp chitosan with a degree of deacetylation of 73%, add it to 1 liter of 2% lactic acid solution and stir for 8 hours, then filter it, adjust the pH of the solution to 5.5, control the temperature at 45°C, and add chitosan-containing enzyme The complex enzyme with hemicellulase catalyzes the degradation of chitosan. When the reaction solution is dripped into 2% sodium hydroxide solution without turbidity, use NMWL 10kDa ultrafiltration membrane to remove the enzyme, and the solution passing through the 10kDa ultrafiltration membrane is further NMWL The oligosaccharide fraction was separated by ultrafiltration with a 1kDa ultrafiltration membrane. Passed through 10kDa membrane, the component that does not pass through 1kDa membrane, obtains chitooligosaccharide lactic acid salt sample after freeze-drying, yield 23%, average polymerization degree 25, dispersion degree 2.85; After the enzyme, the chitooligosaccharide lactate sample was obtain...

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PUM

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Abstract

The invention relates to a process for preparing water-soluble chitooligosacchrides, which is prepared by utilizing the difference of recognition capability and disengagement capability of enzymes to different glycosidic bonds and the inhomogeneous distribution of acetylaminos in chitosan raw material. The invention realizes simple process, wherein at the same time of preparing Chitosan oligosaccharide, 8-200 polymerization degree of water-soluble chitooligosacchride can be achieved simultaneously through the method of ultrafiltration membrane separation. The product obtained thereby has exhibites antineoplastic action and immunologic enhancement.

Description

technical field [0001] The invention relates to a method for preparing water-soluble chitooligosaccharides, in particular to a method for preparing water-soluble chitooligosaccharides with a polymerization degree of 8 to 200. Background technique [0002] As a natural polysaccharide, chitosan, especially the large amount of amino groups, is an alkaline polysaccharide, so it has some unique functions. Studies have revealed that water-soluble chitooligosaccharides and chitooligosaccharides with a degree of polymerization greater than 6 have better anti-tumor effects and immune-promoting effects than corresponding oligosaccharides. When the so-called chitin (degree of deacetylation is less than 20%) and chitosan (degree of deacetylation greater than 70%) are greater than 7, they directly affect digestion and absorption in the body because of their poor water solubility; , many researchers are committed to the research of high degree of polymerization water-soluble chitosan oli...

Claims

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Application Information

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IPC IPC(8): B01D71/08C08B37/08
Inventor 杜予民覃彩芹肖玲李瑾
Owner WUHAN UNIV
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