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Vaccine for inducing specific immunity of tumor and application thereof

A tumor-specific, vaccine-based technology, applied in the field of tumor treatment and prevention, can solve problems such as poor clinical effects, achieve better results, prevent tumor recurrence, and improve anti-tumor activity

Inactive Publication Date: 2013-05-22
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the safety of replication-selective oncolytic viruses alone is good, but their clinical effect is poor, improving the therapeutic effect of these oncolytic viruses is urgent

Method used

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  • Vaccine for inducing specific immunity of tumor and application thereof
  • Vaccine for inducing specific immunity of tumor and application thereof
  • Vaccine for inducing specific immunity of tumor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0045] Example 1: Effects of HPD-1NR and HaK golden hamster tumor models treated with oncolytic virus combined or alone in vivo

[0046] Will 1×10 6 HPD-1NR or 5×10 6 HAK cells were inoculated subcutaneously on the right dorsal side of 6-8 week-old golden hamsters. Ten days later, when the tumor diameter reached 6-7mm, they were divided into five groups, with 8 golden hamsters in each group. , 2, 4, 6, 8 and 10 days with intratumoral injection of PBS; group B with intratumoral injection of 5×10 8 PFU adenovirus Ad5; Group C was intratumorally injected with 5×10 7 PFU vaccinia virus VVLister; group D was injected with 5×10 8 PFU adenovirus Ad5 followed by 5×10 injections on days 6, 8 and 10 7 PFU vaccinia virus VVLister; Group E was injected with 5×10 7 PFU of vaccinia virus VVLister followed by 5×10 injections on days 6, 8 and 10 8 PFU adenovirus Ad5. The injection volume of PBS, adenovirus Ad5, and vaccinia virus VVLister is 100 microliters each time.

[0047] T...

example 2

[0049] Example 2: Sequential immunization with oncolytic adenovirus and vaccinia virus induces lymphocyte infiltration and tumor cell apoptosis in tumors.

[0050] As in Example 1, the golden hamsters were killed on the 10th day after the last virus treatment, and the tumor tissues were collected for further histological and immunohistochemical analysis to observe the infiltration of immune cells and tumor cells in different groups of tumor tissues. of apoptosis. The results showed that in the adenovirus and vaccinia virus sequential immunotherapy group on day 10, there were a large number of infiltrating lymphocytes in the tumor ( Figure 5 ), inducing more tumor cell apoptosis ( Image 6 ).

example 3

[0051] Example 3 : Comparison of humoral and tumor-specific immune responses induced by sequential immunization with oncolytic adenovirus and vaccinia virus, reverse combination, or viral therapy alone.

[0052] As in the treatment method of Example 1, on the 10th day after the virus treatment, the golden hamster was sacrificed and serum and spleen were collected. Humoral and tumor-specific immune responses induced by sequential combination of oncolytic adenovirus and vaccinia virus, reverse combination, or virus alone were examined.

[0053] Detection of total antibodies in serum: spread A549 or CV1 cells in a 96-well plate, dilute the serum by 2 times on the second day, then add adenovirus or vaccinia virus (100PFU), culture in the incubator for one week, observe each Cytopathy in each well. Detection of tumor-specific immune response: After the spleen cells were isolated in vitro, tumor cells treated with mitomycin C (2 mg / ml) were added to stimulate HPD1NR. Three days l...

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Abstract

The invention relates to a vaccine comprising an adenovirus vector and a vaccinia virus vector and an application of the vaccine in treating tumor. The vaccine comprises oncolytic adenovirus and vaccinia virus; the two viruses are used in sequence, wherein the oncolytic adenovirus is the reproductive human adenovirus type 5 and the vaccinia virus is the vaccinia virus for smallpox vaccine. According to the vaccine, the oncolytic adenovirus and the vaccinia virus are prepared into the vaccine by sequential combination, so the antineoplastic activity can be improved, the stronger cellular immune response can be generated compared with the effect of reversely associated virus or separate virus, the using effect is better and the long-term specific immunity of tumor is shown. The tumor vaccine can eradicate the tumor and generate the long-term specific immunity of tumor and can be used in the treatment of various tumors of the human or animals; and meanwhile, the vaccine can effectively prevent the tumor from relapsing and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of tumor treatment and prevention, in particular to a vaccine composed of two viral vectors and its application in treating tumors. Background technique [0002] Today, tumor has become a major disease that threatens human health. There are about 10 million new tumor patients in the world every year. Although we have a deep understanding of the molecular mechanism of tumor occurrence, development and metastasis, tumor surgical treatment, chemotherapy, Radiotherapy, targeted therapy, and other treatments have also made great progress, but for most solid tumors, the overall survival rate has not improved significantly for decades. Therefore, there is a need to develop new approaches to treat tumors and prevent tumor recurrence after treatment. [0003] Tumor immunotherapy is a potential tumor treatment method, because the mechanism of immunotherapy's anti-tumor effect is completely different from conventional chemother...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/275A61P35/00A61K39/235
CPCA61K39/275A61K39/235A61K35/761C12N2710/10132C12N2710/10134C12N2710/24034A61K39/0011A61K2039/5256A61K2039/545A61K2039/55538C12N2710/10043C12N2710/24143A61K39/12A61P35/00
Inventor 王尧河王鹏举杜攀王盛典黎小珠高冬玲袁明尼克莱蒙
Owner ZHENGZHOU UNIV
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