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Method for separating major monomeric compoent of soybean isoflavone

A technology of soybean isoflavones and monomer components, applied in the direction of purification/separation/stabilization of organic compounds, organic chemical methods, chemical instruments and methods, etc., can solve the problem of not being able to obtain glycosides of soybean isoflavones, and obtaining soybeans that cannot be separated Problems such as isoflavones and conditions are difficult to control, to achieve the effect of promoting refined utilization, mild conditions, and low equipment requirements

Inactive Publication Date: 2004-09-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example: USP 5932221 invented a method of extracting soybean meal with acetone, and then using ice water precipitation to separate genistin in the extract, but this technology cannot separate other monomeric components of soybean isoflavones
USPA 20010010930 discloses a method for preparing soybean isoflavone aglycones by using defatted soybeans as raw materials through steps such as enzymolysis, ultrafiltration, centrifugation, and pickling, but this method cannot obtain soybean isoflavone glycosides, and the process is complicated and the conditions are difficult. control
CN 1375492A discloses a method for extracting soybean isoflavone glucosides and aglycones with different solvents using soybean hypocotyls as raw materials, but it cannot separate different glycosides or different aglycones

Method used

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  • Method for separating major monomeric compoent of soybean isoflavone
  • Method for separating major monomeric compoent of soybean isoflavone

Examples

Experimental program
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Effect test

Embodiment 1

[0034] In this embodiment, a mixture of soybean isoflavones with a content of 40.75% is used as a raw material, 37-49 μm silica gel is used as an adsorbent, and a chloroform-methanol solution of chloroform:methanol=5:1 (volume ratio) is used as an eluting system. The process steps are as follows.

[0035] 1. Sample solution preparation

[0036] The mixture of soybean isoflavones was dissolved in methanol to prepare a methanol solution of soybean isoflavones with a concentration of 1.0 mg / ml.

[0037] 2. Adsorbent treatment and packing

[0038] The adsorbent silica gel was washed with acid (1Mol / L HCl) and neutralized with alkali (1Mol / L NaOH), washed with water to pH 6.8, activated at 110°C for 2 hours, then soaked in chloroform-methanol solution for 24 hours to reach equilibrium, and routinely Wet-load the separation column. The separation column is a glass column of φ24×300mm.

[0039] 3. Sample loading

[0040] Add 10ml of the sample solution to the separation column. ...

Embodiment 2

[0048] In this embodiment, a mixture of soybean isoflavones with a content of 62.52% is used as a raw material, 37-49 μm silica gel is used as an adsorbent, and a chloroform-methanol solution of chloroform:methanol=5:1 (volume ratio) is used as an eluting system. The process steps are as follows.

[0049] 1. Sample solution preparation

[0050] The mixture of soybean isoflavones was dissolved in methanol to prepare a methanol solution of soybean isoflavones with a concentration of 1.5 mg / ml.

[0051] 2. Adsorbent treatment and packing

[0052]The adsorbent silica gel was washed with acid (1Mol / L HCl) and neutralized with alkali (1Mol / LNaOH), washed with water to pH 6.8, activated at 110°C for 2h, then soaked in chloroform-methanol solution for 24h to reach equilibrium, and routinely wet into the separation column. The separation column is a glass column of φ24×300mm.

[0053] 3. Sample loading

[0054] Add 10ml of the sample solution to the separation column.

[0055] 4....

Embodiment 3

[0062] In this embodiment, a mixture of soybean isoflavones with a content of 79.32% is used as a raw material, 37-49 μm silica gel is used as an adsorbent, and a chloroform-methanol solution of chloroform:methanol=5:1 (volume ratio) is used as an eluting system. The process steps are as follows.

[0063] 1. Sample solution preparation

[0064] The mixture of soybean isoflavones was dissolved in methanol to prepare a methanol solution of soybean isoflavones with a concentration of 2.0 mg / ml.

[0065] 2. Adsorbent treatment and packing

[0066] The adsorbent silica gel was washed with acid (1Mol / L HCl) and neutralized with alkali (1Mol / L NaOH), washed with water to pH 6.8, activated at 110°C for 2 hours, then soaked in chloroform-methanol solution for 24 hours to reach equilibrium, and routinely Wet-load the separation column. The separation column is a glass column of φ24×300mm.

[0067] 3. Sample loading

[0068] Add 10ml of the sample solution to the separation column. ...

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Abstract

The invention is a method to separate main monomer components in soy isoflavone, using the mixture with not less than 40% soy isoflavone content as raw material, silica gel as sorbent, and chloroform-methanol solution as eluting system, its technical steps: preparing sample solution; processing sorbent and placing in column; applying sample, where the added quantity of sample solution is 5-15% of effective volume of separating column; eluting, where the eluent flow velocity is controlled at 0.5-1.5% of effective volume of separating column each minute, and collecting the eluent in sections; qualitatively detecting the collected eluents, respectively and compared with the contrast product; obtaining monomer components, combining the eluents with the same component, and respectively vacuum condensing and drying to obtain four main monomer components: genistein, daidzein, genistin and daidzin. It is detected by high efficiency liquid phase chromatography that each monomer component's content can reach above 90%.

Description

technical field [0001] The invention relates to a method for separating and obtaining four main monomer components of genistein, daidzein, genistin and daidzin from soybean isoflavones. Background technique [0002] Soy isoflavones are secondary metabolites of soybean, which are a mixture of various monomer components. According to different chemical structures, soybean isoflavones can be divided into two categories: free aglycon and bound glycoside, of which aglycon accounts for about 2-5% of the total amount, mainly including genistein and daidzein; Glycosides account for about 95-98% of the total, mainly in the form of genistin and daidzin. The chemical structural formulas of the four main monomer components of soybean isoflavones are as follows. [0003] [0004] Genistein Genistein [0005] [0006] daidzein [0007] Soy isoflavones have a variety of biological activities, such as inhibiting lipid peroxidation, scavenging ac...

Claims

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Application Information

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IPC IPC(8): C07B63/00C07D311/30C07H1/06C07H17/07
Inventor 姚开贾冬英何强吕远平潘廖明
Owner SICHUAN UNIV
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