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Method for extracting and separating isoflavone from kudzu slag

A technology for isoflavones and kudzu slag, which is applied in the field of extraction and separation of isoflavones, can solve the problems of low content of puerarin products, low extraction efficiency, long operation period, etc., and achieves huge ecological environment effect, significant social benefit, and short extraction period. Effect

Inactive Publication Date: 2006-05-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems of these processes are that the operation cycle is long, the extraction efficiency is not high, the content of the puerarin product obtained is generally low, and organic solvents such as n-butanol and ethyl acetate are used in the extraction process, which leads to the difficulty of solving the solvent residue problem.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take by weighing 2 kg of dried kudzu slag, pulverize the kudzu slag twice coarsely and finely to obtain a powder of 60 to 100 meshes, and at an extraction temperature of 60° C., use 95% ethanol as an extraction solvent, and the solid-to-liquid ratio of each extraction is 1:2, extraction 3 times, extraction time is 3 hours, extraction 3 times, each extraction time is 1 hour. The extracts were combined, filtered and concentrated under reduced pressure to obtain the total isoflavones with an extraction rate of 86%.

[0026] Put the total isoflavones on a 30-60-mesh polyamide column, and elute with gradients of water and 25-95% ethanol. First, it was eluted with water and followed by TLC. After a fraction comes out, change to 25% ethanol (V / V) solution for elution. The concentration of the eluent is gradually increased, and after the puerarin is eluted, the concentration of the eluent is increased to 60% until it reaches 95%. The eluate rich in puerarin and daidzein were...

Embodiment 2

[0028] Weigh 2 kg of dried kudzu slag, pulverize the kudzu slag twice coarsely and finely to obtain a powder of 100 to 150 meshes, and at an extraction temperature of 45° C., use 70% ethanol as an extraction solvent, and the solid-to-liquid ratio of each extraction is 1:3, extraction 3 times, extraction time is 4 hours, respectively 2 hours for the first time, 1 hour for the second and third times. The extracts were combined, filtered and concentrated under reduced pressure to obtain the total isoflavones with an extraction rate of 89%.

[0029] Put the total isoflavones on a 30-60-mesh polyamide column, and elute with gradients of water and 25-95% ethanol. First, it was eluted with water and tracked by TLC. After a fraction comes out, change to 25% ethanol (V / V) solution for elution. The concentration of the eluent was gradually increased, and after the puerarin basically eluted, the concentration of the eluent was increased to 50% until it was increased to 95%. Collect th...

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Abstract

The invention discloses a process for extracting and separating isoflavones from kudzu slag, which comprises the steps of disintegrating kudzu slag to 60-150 meshes, loading into a liquid dynamic continuous flow upstream extraction device for alcohol extraction, combining the extract liquid and filtering, concentrating the filtrate to obtain total isoflavones extract, and separating the total isoflavones extract to obtain puerarin and soybean aglycone.

Description

(1) Technical field [0001] The invention relates to a method for extracting and separating isoflavones from kudzu slag (2) Background technology [0002] The extraction of flavonoids was not considered when starch was extracted from fresh Pueraria lobata, and the residues were discarded, but a large part of the active ingredients in Pueraria lobata remained in the residue. The main active ingredients are puerarin and daidzein. In recent years, a large number of literatures at home and abroad have reported the pharmacological effects of its active ingredients. Puerarin has been used as a raw material to make medicines and is widely used clinically. Puerarin is used to treat angina pectoris, hypertension, myocardial infarction, arrhythmia, and hyperviscosity; It has an expansion effect, and it also has important effects on inhibiting cancer cells and anti-aging. In particular, at present, puerarin has become one of the first-choice drugs for cardiovascular and cerebrovascul...

Claims

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Application Information

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IPC IPC(8): C07D311/36
Inventor 苏为科李坚军
Owner ZHEJIANG UNIV OF TECH
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