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Method for extracting high-purity ursolic acid from rosemary

A technology for ursolic acid and rosemary, applied in the field of extracting high-purity ursolic acid, can solve the problems of unsuitability for industrialized production, harsh separation conditions, low production capacity, etc., and achieves long production cycle, reduced production cost, and production capacity. low effect

Active Publication Date: 2013-05-15
ZHONGSHAN BAILING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing chromatographic separation method has low production capacity, harsh separation conditions, and long production cycle, and is not suitable for industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present invention extracts the method for high-purity ursolic acid from rosemary, comprises the following steps:

[0025] A, 1kg of rosemary leaf leftovers after extracting carnosic acid, add 15L ethanol to extract 1 time, 0.5 hour, filter, get filtrate, add activated carbon decolorization in filtrate, filter, concentrate to 0.2L, add 2 under stirring Double the volume of water to precipitate and precipitate, filter to obtain the crude extract of ursolic acid; the purity is 35-40%;

[0026] B, add acetone to dissolve in the crude extract of ursolic acid in the step A, add diethylamine reflux reaction to form a salt, be cooled to room temperature, filter, obtain amine salt;

[0027] C. Add the amine salt in step B into water to dissolve it, add isopropyl ether, adjust the pH value to 2-5 with dilute hydrochloric acid, maintain 20°C for one hour, filter, and dry the filter cake; the purity is 90%;

[0028] D. Add methanol to dissolve the filter cake after drying in s...

Embodiment 2

[0030] The present invention extracts the method for high-purity ursolic acid from rosemary, comprises the following steps:

[0031] A, 0.25kg rosemary extracts carnosic acid leftovers, add 8L ethanol to extract 4 times, each time for 3 hours, filter to get the filtrate, add activated carbon to the filtrate for decolorization, filter, concentrate to 3L, add 5 Double the volume of water to precipitate and precipitate, filter to obtain the crude extract of ursolic acid; the purity is 35-40%;

[0032] B, add methyl isobutyl ketone to dissolve in the crude extract of ursolic acid in the step A, triethylamine reflux reaction becomes salt, cool room temperature, filter, obtain amine salt;

[0033] C. Add the amine salt in step B into water to dissolve it, add isopropyl ether, adjust the pH value to 2-5 with dilute phosphoric acid, maintain 40°C for one hour, filter, and dry the filter cake; the purity is 90%;

[0034] D. Add methanol to dissolve the filter cake after drying in step...

Embodiment 3

[0036] The present invention extracts the method for high-purity ursolic acid from rosemary, comprises the following steps:

[0037] A. Extract carnosic acid from 1kg of rosemary leaves, add 20L ethanol to extract 3 times, each time for 2 hours, filter to obtain the filtrate, add activated carbon to the filtrate for decolorization, filter, concentrate to 5L, add to the filtrate while stirring Precipitate with 4 times the volume of water, filter to obtain the crude extract of ursolic acid; the purity is 35-40%;

[0038] B, add tert-amyl alcohol to dissolve in the ursolic acid crude extract in step A, add diethylamine reflux reaction and become salt, cool room temperature, filter, obtain amine salt;

[0039] C. Add the amine salt in step B into water to dissolve it, add isopropyl ether, adjust the pH value to 2-5 with dilute sulfuric acid, maintain 30°C for one hour, filter, and dry the filter cake; the purity is 90%;

[0040] D. Add methanol to dissolve the filter cake after d...

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PUM

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Abstract

The invention discloses a method for extracting high-purity ursolic acid from rosemary, which is characterized by comprising the following steps of: A, adding ethanol into the leftovers obtained after grinding rosemary leaves and extracting carnosic acid for extraction; filtering and obtaining the filtrate; adding active carbon into the filtrate for decoloration; filtering and concentrating; adding 2-5 times water by volume while stirring to separate out precipitate; filtering to obtain a crude extract of ursolic acid; B, adding an organic solvent into the crude extract of ursolic acid in the step A for dissolution; adding diethylamine or triethylamine for backflow reaction to generate salt; and cooling to room temperature and filtering to obtain amine salt; C, adding water into the amine salt in the step B to dissolve the amine salt; adding isopropyl ether; adjusting the pH value to 2-5 by dilute acid; keeping the temperature at 20-40 DEG C for 1 hour; filtering; and drying the filter cake; and D, adding methanol into the dried filter cake in the step C for dissolution; adding neutral alumina for decoloration; filtering and concentrating the filtrate; cooling to minus10-20 DEG C for crystallization; and centrifuging, washing and drying to obtain the finished product of ursolic acid.

Description

technical field [0001] The invention relates to a method for extracting high-purity ursolic acid from rosemary. Background technique [0002] Rosemary (Rosmarinus offoconalis L.) is a plant of the genus Rosmarinus of the Lamiaceae, rich in a variety of efficient and safe antioxidant components. Rosemary extract is a new type of excellent natural antioxidant, which can effectively remove free radicals in the body, prevent food from going bad, and has anti-inflammatory, anti-tumor, and anti-HIV effects. [0003] Ursolic acid is a triterpenoid compound present in natural plants, which has various biological effects such as sedative, anti-inflammatory, antibacterial, anti-diabetic, anti-ulcer, hypoglycemic, etc. Ursolic acid also has obvious antioxidant function , and thus is widely used as a raw material for medicine and cosmetics. [0004] Because rosemary plant or carnosic acid leftovers contain a large amount of oleanolic acid and ursolic acid, oleanolic acid and ursolic a...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 梁待亮周海香张和平刘遗松欧松黄文军沈惠宾霍延豪阮宇锋刘骅
Owner ZHONGSHAN BAILING BIOTECHNOLOGY CO LTD
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