129 results about "Enzyme activator" patented technology

The invention provides methods for treating or decreasing the likelihood of developing a stress-granule related disorder and / or cancer by administering one or more poly-ADP-ribose polymerase (PARP) inhibitors, one or more PARP activators, one or more poly-ADP-ribose glycosylase (PARG) activators, and / or one or more poly-ADP-ribose glycohydrolase ARH3 activators. The invention also provides corresponding methods of decreasing stress granule formation and / or proliferation in a cell or a population of cells. The invention further provides methods of increasing the number of stress granules and proliferation in a cell or a population of cells by administering one or more PARP activators, one or more PARP inhibitors, one or more PARG inhibitors, and / or one or more ARH3 inhibitors. The invention also provides methods for screening for agents for treating or decreasing the likelihood of developing a stress granule-related disorder or cancer, and methods for determining the propensity for developing a stress granule-related disorder or cancer, as well as compositions and kits containing one or more PARP inhibitors, one or more PARP activators, one or more PARG activators, and one or more ARH3 activators.

Disclosed is a multifunctional color bar enzymatic toothpaste and preparation method. The toothpaste is formed by one color regulation cream which containing chemical medicament additive and biologic enzyme activator, and multi-color cream containing biologic enzyme and biologic enzyme stabilizer. The preparation method is separately preparing regulation cream and enzyme cream and canning to color bar toothpaste. Functions of the toothpaste is to prevention and cure oral problem such as preventing and curing decayed tooth, diminishing inflammation, sterilization, desensitization, eliminating halitosis and whitening teeth. Simultaneously the toothpaste employing stabilizer and color bar to ensure the stability, and effectively exerting biology function of enzyme in release process by adding definite activator.

The invention relates to the field of an external diagnostic reagent, and particularly relates to a high-sensitivity kit for detecting 5'-nucleotidase. The kit for detecting 5'-nucleotidase comprises a 5'-nucleotidase R1 reagent, a 5'-nucleotidase R2 reagent and a 5'-nucleotidase calibration product, wherein the 5'-nucleotidase R1 reagent comprises a buffer solution, a stabilizing agent, a preservative agent, an enzyme activator, 4-AAP(4-aminoantipyrine), 18-crown ether-6 and UAO (Uricase), PNP (Purine Nucleoside Phosphorylase), XOD (Xanthine Oxidase) and POD (Peroxidase) reaction enzyme systems; the 5'-nucleotidase R2 reagent comprises a buffer solution, a stabilizing agent, a preservative agent, PO3- and a Trinder developing system; and the 5'-nucleotidase calibration product comprises a buffer solution, a stabilizing agent, a preservative agent and 5'-nucleotidase. The detection kit provided by the invention is enhanced in detection sensitivity by 40% compared with a 'PNP-XOD-POD' enzyme system, and has good application prospects.

The present invention belongs to the field of food-derived peptides and discloses a collagen peptide, a production method, a production device and an application thereof. The production method of thecollagen peptide comprises steps of coarse collagen solution heating, cooling, high-efficiency compound enzyme preparation enzymatic hydrolyzing, membrane filtering, concentrating, ultra-high pressurehomogenizing, and ultra-high temperature instantaneous sterilizing and drying. The high-efficiency compound enzyme preparation enzymatic hydrolyzing uses a compound enzyme preparation comprising compound protease, alkaline protease, neutral protease, flavor protease, animal proteolytic enzyme and an enzyme activator, and the enzyme activator is dithiothreitol and / or beta-mercaptoethanol. The provided method does not need to adjust pH value of the enzymatic hydrolyzing, does not need to control enzymatic hydrolyzing temperatures, is short in enzymatic hydrolyzing time and small in enzyme addition amount, and enables the enzymatic hydrolyzed product to be low and concentrated in molecular weight. The prepared collagen peptide powder is free of fishy smell, extremely excellent in water resolubility and also not liable to absorb moisture and agglomerate during storage process, and can be used in solid beverages, effervescent tablets, chewable tablets, health-care foods and feature-beautifying and skin-protecting products.

The invention discloses a preparing method of feeding enzymolytic protein and shrimp case powder through waste shrimp head in the feeding additive preparing domain, which comprises the following steps: (1) squeezing the shrimp head to separate the case from meat; (2) breaking case and liquid meat; homogenizing; adding water to soak; (3) adding pH value buffer and enzyme activator; heating; stirring continuously; (4) condensing the filtrate; drying; obtaining the product. The invention saves cost to accelerate the manufacturing speed with high amino acid rate and stable quality, which utilizes the waste shrimp head sufficiently to avoid environment pollution.

The present invention relates to biological technology, and is especially biological enzyme activator for promoting the activity of biological enzyme. The biological enzyme activator has acetic acid as carrier and contains mixing of Ca ion in 14-18 wt%, Mg ion in 6.5-9 wt% and Mn ion in 0.5-1 wt%. The biological enzyme activator may find its wide application in agriculture, animal husbandry and other industry.

The invention discloses an environment-friendly straw fermenting agent and a preparation method thereof. The environment-friendly straw fermenting agent is prepared from the following raw materials inparts by weight: 26 to 30 parts of bacillus subtilis, 21 to 25 parts of trichoderma viride, 10 to 14 parts of saccharomyces cerevisiae, 10 to 14 parts of aspergillus niger, 1 to 5 parts of pectinase,5 to 8 parts of amylase, 1 to 3 parts of rayleigh mold, 5 to 10 parts of acid protease, 5 to 10 parts of neutral protease, 8 to 15 parts of arthrobacterium, 5 to 10 parts of cellulase, 1 to 3 parts of anti-aging agent, and 1 to 3 parts of composite fungicide, wherein the composite fungicide is prepared from the following raw materials in parts by weight: 1 to 3 parts of low-temperature cellulase-producing bacteria solution, 1 to 2 parts of normal-temperature cellulase-producing bacteria solution, 1 to 2 parts of normal-temperature amylomyces-producing bacteria solution, 2 to 4 parts of normal-temperature acetic bacteria-producing solution, 1 to 3 parts of normal-temperature yeast bacteria solution, and 3 to 5 parts of enzyme activator. The fungicide can accelerate the rapid fermentation of straws under a low-temperature condition, and the microorganisms of the fungicide have good synergistic reaction to have high-efficiency degradation performance for the straw which is difficult to degrade.

The invention discloses a rapid library building method for identifying a target protein chromatin binding map, which comprises the following steps: collecting sample cells, adding activated magneticbeads into the cells, incubating, and incubating the magnetic beads with a primary antibody; pre-binding a secondary antibody with recombinant fusion transposase; incubating the magnetic beads with the pre-combined secondary antibody and recombinant fusion transposase, adding a transposase activator and a cell perforating agent into the magnetic beads, and incubating; and terminating the transposase reaction, and carrying out library amplification. According to the method, the process of a traditional method is remarkably simplified, the library building time is shortened, the library buildingefficiency is improved, the loss is small, and the requirement for library building materials is lower. Meanwhile, three DNA standard substances with different concentrations are doped into the library building process, so that the effect of accurately quantifying the DNA copy number in the library can be achieved. The method is very suitable for researching a small amount of precious samples orsamples with different treatment states.

Peptides that increase melanin synthesis are provided. These peptides include pentapeptides YSSWY, YRSRK, and their variants. The peptides may activate the enzymatic activity of tyrosinase to increase melanin synthesis. The pharmaceutical, cosmetic, and other compositions including the peptides are also provided. The methods of increasing melanin production in epidermis of a subject are provided where the methods include administering compositions comprising an amount of one or more peptides effective to increase the melanin production. The methods also include treating vitiligo or other hypopigmentation disorders with compositions including one or more peptides.

This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant density, and by affinity purification.

The invention discloses spinosyn A and a derivative thereof as an activator of argininosuccinate synthetase 1 (ASS1) and an activator of mutant argininosuccinate synthetase ASS1G362V and application of the Spinosyn A and the derivative thereof. The spinosyn derivative has a structural general formula (I). The spinosyn and the derivative thereof are used as a medicine and an anti-tumor medicine fortreating I-type citrullinemia by activating the argininosuccinate synthetase 1 (ASS1) and the mutant argininosuccinate synthetase ASS1G362V in a targeted manner.

The invention discloses a bio-enzyme-based efficient water purifying agent. The water purifying agent is prepared from the following components in parts by weight: 30-35 parts of bio-enzyme, 10-12 parts of microorganism, 5-8 parts of microbial flocculant, 5-8 parts of moringa seed flocculant, 3-5 parts of bio-enzyme preparation, 10-12 parts of chitin, 3-5 parts of chitosan, 5-8 parts of sodium carboxymethyl starch, 1-3 parts of functional oligopeptide, 5-8 parts of white polyaluminum chloride, 20-25 parts of water, and 20-25 parts of filler. The bio-enzyme-based efficient water purifying agent has the beneficial effects that the functional oligopeptide can be combined with enzyme so as to serve as an enzyme activator, thereby improving enzyme activity, allowing enzyme to degrade a substrate faster, and remarkably improving the water purification efficiency of the water purifying agent; and a small amount of water purifying agent can remove a wide range of impurities in to-be-purified water, the water purifying agent has high purification efficiency, and leaves no residual toxic or subsidiary substance, therefore, the bio-enzyme-based efficient water purifying agent has high economic and social benefits.

The invention discloses a maifanite-containing double-effect multi-component organic / inorganic compound mixed fertilizer and a manufacturing method thereof. The maifanite-containing double-effect multi-component organic / inorganic compound mixed fertilizer is low-carbon and environment-friendly and produced by organic combination of natural components including humic acid, maifanite, bentonite, potassium oxide, ammonium phosphate, ammonium chloride, borax, zinc sulfate, aluminum potassium sulfate, and traditional Chinese medicines for agricultural use (including aloe, Tripterygium wilfordii, onion juice, tobacco stem, Radix Glycyrrhizae, Aconitum kusnezoffii and Pyreyhrum), biological enzyme activators and a PASP plant growth promoter at normal temperature and pressure. The manufacturing method comprises the following steps: grinding multiple raw materials in the formula to powder of about 120 to 200 meshes, thoroughly mixing according to the given ratio in a stirring machine, conveying to a granulating machine through a conveyor, chelating by spraying a PASP aqueous solution and curing and drying at a high temperature by use of a drying machine, sieving to obtain the fertilizer product which has a diameter of 2.5 to 4.5 mm, a strength of 5 to 6 n and a free moisture of less than 10%, and quantitatively bagging after the product passes the quality test. The fertilizer product has complete nutrients and balanced nutrition, is rich in organic matters and amino acids, can activate and utilize residual fertilizers in the soil, improves yield and quality of crops, produces green and organic agricultural products, helps increase farmer's incomes, well solves the problems and difficulties caused by long-term use of chemical fertilizers in agricultural production and farming, overcomes the defects of chemical fertilizers, and truly meets the national industrial policy requirements for economic and social benefits of green, organic and environment-friendly sustained- / controlled-release fertilizers.