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Kit for detecting pepsinogen II

A pepsinogen and kit technology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of poor sensitivity, small amount of detection samples, and long measurement time, and achieves both detection sensitivity and linear range. Effects of specificity, improved stability and sensitivity

Inactive Publication Date: 2018-12-11
广州市伊川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In enzyme-linked immunosorbent assay, its detection sensitivity is high, but the operation process is slightly complicated, the determination time is long, and there are many influencing factors, and it is not suitable for the detection of emergency samples; in the determination of chemiluminescence immunoassay, its automatic operation speed Fast, high accuracy, but high cost, expensive equipment, poor stability, unable to popularize middle and primary hospitals and medical institutions; in latex immunoturbidimetric assay, its detection is fast, accurate, high sensitivity, strong specificity, precision The accuracy is good, the cost is low, and the test sample volume is small, which can be used for the determination of a small number of samples and emergency samples
[0005] However, the existing latex immunoturbidimetric method has some shortcomings: poor stability, poor sensitivity

Method used

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  • Kit for detecting pepsinogen II
  • Kit for detecting pepsinogen II
  • Kit for detecting pepsinogen II

Examples

Experimental program
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Effect test

Embodiment 1

[0030] This embodiment provides a kit for detecting pepsinogen II, including reagent R1 and reagent R2; reagent R1 is composed of the following components: phosphate buffer 150mmol / L, Tris-HCl buffer 50mmol / L , Triton X-100 1g / L, polyethylene glycol 6000 8g / L and Proclinc 300 0.5g / L, the concentration of components in reagent R1 is the final concentration of components in reagent R1; reagent R2 is composed of the following concentrations Composition: phosphate buffer 150mmoL / L, glycerin 80g / L, calf serum albumin 20g / L, anti-human pepsinogen II antibody latex solution 1.5g / L and Proclinc-300 0.5g / L, reagent R2 The component concentration is the final concentration of the component in reagent R2.

[0031] The above-mentioned anti-human pepsinogen II antibody latex solution is prepared by the following method:

[0032] S1) Get 3mL of carboxylated latex microsphere solution, dilute to 6mL with phosphate buffer; The mass percent of carboxylated latex microspheres is 5%;

[0033]...

Embodiment 2

[0038] This embodiment provides a kit for detecting pepsinogen II, including reagent R1 and reagent R2; reagent R1 is composed of the following components: phosphate buffer 150mmol / L, Tris-HCl buffer 50mmol / L , Triton X-100 1g / L, polyethylene glycol 6000 8g / L and Proclinc 300 0.5g / L, the concentration of components in reagent R1 is the final concentration of components in reagent R1; reagent R2 is composed of the following concentrations Composition: phosphate buffer 150mmoL / L, trehalose 80g / L, calf serum albumin 20g / L, anti-human pepsinogen II antibody latex solution 1.5g / L and Proclinc-300 0.5g / L, reagent R2 The middle component concentration is the final concentration of the component in reagent R2.

[0039] The above-mentioned anti-human pepsinogen II antibody latex solution is prepared by the following method:

[0040] S1) Get 3mL of carboxylated latex microsphere solution, dilute to 6mL with phosphate buffer; The mass percent of carboxylated latex microspheres is 5%; ...

Embodiment 3

[0046] This embodiment provides a kit for detecting pepsinogen II, including reagent R1 and reagent R2; reagent R1 is composed of the following components: MOPS-NaOH buffer solution 10mmoL / L, Tween 20 0.01g / L , polyethylene glycol 80005g / L and sodium azide 0.05g / L, the component concentration in the reagent R1 is the final concentration of the component in the reagent R1; the reagent R2 is composed of the following components: MOPS-NaOH buffer solution 160mmoL / L, sucrose 10g / L, casein 5g / L, anti-human pepsinogen II antibody latex solution 0.5g / L and sodium azide 0.05g / L, the concentration of components in reagent R2 is the final concentration of components in reagent R2 .

[0047] The above-mentioned anti-human pepsinogen II antibody latex solution is prepared by the following method:

[0048] S1) Get 3mL carboxylated latex microsphere solution, dilute to 6mL with phosphate buffer; Carboxylated latex microsphere is polystyrene latex microsphere, the particle diameter of carb...

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Abstract

The invention discloses a kit for detecting pepsinogen II, belonging to the technical field of biological detection. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises components of the following concentrations: buffer of 10-200mmoL / L, surfactant of 0.01-5g / L, accelerator of 5-20g / L, and preservative of 0.05-2g / L, wherein a component concentration in the reagent R1 is the final concentration of a component in the reagent R1; reagent R2 comprises the components of the following concentrations: buffer of 80-160mmoL / L, stabilizer of 10-120g / L, blocking agent5-30g / L, anti-human pepsinogen II antibody latex solution of 0.5-3.0g / L, and preservative of 0.05-2g / L, wherein a component concentration in the reagent R2 is the final concentration of a componentin the reagent R2; the stabilizer is at least one of glycerin, sucrose, trehalose and glucose. The kit of the invention has the advantages of high sensitivity, high precision, stable reagents and thelike.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a kit for detecting pepsinogen II. Background technique [0002] Pepsinogen (Pepsinogen, PG) is an inactive precursor of pepsin in gastric juice, a single-chain polypeptide with a molecular weight of 42000Da, which can be converted into active pepsin in the stomach. According to biochemical properties, immunogenicity, cell origin and tissue distribution, pepsinogen can be divided into two subgroups, pepsinogen Ⅰ (PGⅠ) and pepsinogen Ⅱ (PGⅡ). It is called PGⅠ (PGA), which is mainly secreted by the principal cells of the fundic gland and cervical mucous cells; 6-7 components are called PGⅡ (PGC), except that it is secreted by the above two cells, the mucus cells of the pyloric gland, cardia Gland and the Brunner gland in the upper part of the duodenum can also produce PGII, and the PGII synthesized by the gastric mucosa is about 25% of the total. [0003] Under norma...

Claims

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Application Information

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IPC IPC(8): G01N33/573
CPCG01N33/573G01N2333/96477
Inventor 刘光华杨玉军刘秋明许翠
Owner 广州市伊川生物科技有限公司
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