Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Diagnostic reagent for detecting cow subclinical mastitis and preparation method thereof

A technology of subclinical mastitis and diagnostic reagents, applied in the field of veterinary inspection, can solve the problems of high toxicity, etc., and achieve the effects of stable diluent, stable product and high diagnostic sensitivity

Inactive Publication Date: 2010-09-08
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent uses the highly toxic sodium azide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

experiment example

[0031] The diagnostic coincidence rate test of the present invention

[0032] 1 test material

[0033] Diagnostic solution (prepared and diluted according to the aforementioned method), CMT diagnostic solution (imported from British COX), milk cell counter (Bently, USA), binocular biological microscope (domestic XSZ-107), glass slide, Loefflers methylene blue stain liquid, xylene, 95% ethanol.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a diagnostic reagent for detecting subclinical mastitis of mammals such as cows, sheep, pigs and the like and a preparation method for the reagent. The detection reagent is formed by the following components by mass percent: 8.8-9.2 percent of lauryl sodium sulfate, 4.8-5.2 percent of linear alkyl benzene sulfonic acid, 0.5-0.6 percent of boric acid, 0.04-0.08 percent of bromcresol purple, 0.6-0.8 percent of sodium hydroxide and remaining percent of water. The preparation method is that lauryl sodium sulfate, linear alkyl benzene sulfonic acid and boric acid are mixed first, a little of water (proper amount) is added for agitation to enable lauryl sodium sulfate, linear alkyl benzene sulfonic acid and boric acid to be fully dissolved, then bromcresol purple aqueous solution is added and is fully and evenly agitated, and finally sodium hydroxide aqueous solution and water are added in the obtained solution.

Description

technical field [0001] The invention relates to a veterinary inspection technology, specifically a diagnostic reagent for detecting subclinical mastitis in mammals such as cattle, sheep and pigs, and is particularly suitable for detecting subclinical mastitis in cows. Background technique [0002] Dairy cow mastitis is one of the common and frequently-occurring diseases of dairy cows. It can be divided into clinical mastitis and recessive mastitis according to whether there are clinical symptoms, most of which are recessive mastitis. Dairy cows suffering from subclinical mastitis have reduced productivity, and their losses account for about 10% of the total annual production capacity; and the total amount of amino acids in milk is reduced by 21%, valine, methionine, isoleucine, leucine, 6 kinds of essential amino acids such as alanine and lysine are significantly reduced; lactose, casein, fat, Ca in milk 2+ ,P 3- 、K + The content of beneficial ingredients such as milk als...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/52G01N21/82G01N21/78
Inventor 罗金印李新圃李宏胜郁杰张礼华王兴亚
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com