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Enterococcus faecium and application thereof

A technology of Enterococcus faecium and Escherichia coli, which is applied in the direction of bacteria, medical preparations containing active ingredients, microorganisms, etc., can solve the problems of not being widely used as feed additives, acid resistance, high temperature difference, etc., and achieve enhanced non-specific immune function , strong stress resistance, promote digestion and absorption

Active Publication Date: 2013-03-20
北京龙科方舟生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Enterococcus faecium (Enterococcus Faecium) widely exists in nature and in the gastrointestinal tract of livestock and poultry. It is a beneficial microorganism. Common Enterococcus faecium has not been widely used in feed additives due to its poor acid resistance and high temperature resistance.

Method used

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  • Enterococcus faecium and application thereof
  • Enterococcus faecium and application thereof
  • Enterococcus faecium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Example 1, Isolation and Identification of Enterococcus Faecium Kang 80

[0050] The enterococcus faecium of the invention is obtained by extracting, separating, screening and purifying from feed samples.

[0051] (1) Isolation and purification of Enterococcus faecium Kang 80 (NFER-5)

[0052] 1.1 Isolation and cultivation of strains

[0053]Take 1g of feces from healthy weaned piglets and put them in a test tube filled with 9mL of sterile normal saline, vortex and oscillate to make a 1:10 dilution, and then take the dilution for ten-fold incremental dilution, and then choose 3 appropriate gradient dilutions 1mL of each solution was spread on MRS agar medium and M17 agar medium containing 10ppm cycloheximide, anaerobically cultured at 37°C for 48-72h, the colony morphology was observed and recorded, and characteristic single colonies with good growth were picked and cultured in MRS liquid In the base, streak separation and purification were carried out. Observe wheth...

experiment example 2

[0126] Experimental Example 2, Application of Enterococcus Faecium Kang 80CGMCC No.5058

[0127] First, the impact on piglet performance

[0128] 1.1 Materials and methods:

[0129] experimental animals

[0130] A total of 100 Du long three-way hybrid piglets weaned on 28±2 days were selected, with an average weight of 9.0±0.32kg. They were randomly divided into 2 treatment groups according to body weight, with 5 replicates in each group and 10 piglets in each replicate.

[0131] 1.2 Culture of Enterococcus faecium Kang 80

[0132] Enterococcus Faecium (Enterococcus Faecium) Kang 80CGMCC No.5058 was inoculated into the MRS medium at an inoculation amount of 1%, and cultured at 37°C for 20 hours to obtain a culture of Kang 80.

[0133] 1.3 Test grouping

[0134] The experiment was divided into two treatments. Treatment 1 was the test group, and 100 g / t of Kang 80 culture was added to the basal diet; treatment 2 was the control group (only basal ration). The composition and...

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Abstract

The invention discloses an enterococcus faecium and application thereof. An enterococcus faecium strain provided by the invention is enterococcus faecium suppressant 80, and the collection number is CGMCC No.5058. As proved by experiments, the enterococcus faecium suppressant 80 is obtained by separating, identifying and screening; and the enterococcus faecium suppressant 80 has high resistance, high bacterial resistance and probiotic characteristics, and can be taken as an additive for preparing animal feeds; and the animals include but is not limited to various animals such as pigs, cows, sheep, chicken and the like. The feeds have similar functions to antibiotic feeds, but do not have the side effects of the antibiotic feeds.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Enterococcus faecium and its application. Background technique [0002] While the addition of antibiotics in feed has made a significant contribution to the development of intensive animal husbandry, its side effects such as endogenous infection and superinfection, the emergence of drug resistance, the destruction of normal intestinal flora and the Residues, etc. pose a serious threat to aquaculture, animal and human food safety. Prebiotics are mainly living microorganisms. After animals ingest a certain amount, a certain number of strains can adhere, colonize and grow in specific parts of the gastrointestinal tract. Mainly by regulating the structure and balance of intestinal microbial flora, the animal The preparation that plays a beneficial role in growth and health is a new green and safe additive that is a good alternative to antibiotics. [0003] Enterococcus faec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01A61K35/744
Inventor 谯仕彦丁修良王炜宏宋青龙
Owner 北京龙科方舟生物工程技术有限公司
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