Preparation method for separating and extracting new compound from A. terreus secondary metabolite
A technology for secondary metabolites and compounds, applied in the field of separation and extraction of new compounds, can solve the problem that secondary metabolites are not new secondary metabolites, etc., and achieve the effects of improving extraction rate and accelerating the release of intracellular substances
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[0020] Example 1:
[0021] A preparation method for separating and extracting new compounds from A. terreus secondary metabolites, including spore preparation, strain fermentation, secondary metabolite extraction, and separation and purification. The specific steps are:
[0022] Spore preparation: Place the glycerol cryotube of the strain A.terreus OUCMDZ-2739 (containing 20% glycerol and 3.3% seawater in a freshly prepared PDA liquid cryopreservation solution) stored in an ultra-low temperature refrigerator at -80°C in a clean operating table. Resuscitate for 10 minutes. Dip the resuscitated cryopreservation solution with the inoculating loop after burning and sterilization, and streak evenly in the freshly prepared PDA slope solid medium, and then place the slope medium in a 28℃ constant temperature incubator 4 Day, cultivate the strain slant to mature, get plump spores, set aside;
[0023] Strain fermentation: Add the epigenetic modification regulator TSA to the fungal culture ...
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[0031] Example 2:
[0032] A preparation method for isolating and extracting new compounds from A. terreus secondary metabolites, including spore preparation, strain fermentation, secondary metabolite extraction, and separation and purification. The specific steps are:
[0033] Spore preparation: Place the glycerol cryopreservation tube of strain A.terreus OUCMDZ-2739 (containing 20% glycerol and 3.3% seawater in a freshly prepared PDA liquid cryopreservation solution) stored in an ultra-low temperature refrigerator at -80°C in a clean operating table. Resuscitate for 10 minutes. Dip the resuscitated cryopreservation liquid with the inoculating loop after burning and sterilization, and streak evenly in the freshly configured PDA slope solid medium, then place the slope medium in a 28℃ constant temperature incubator for 3 Day, cultivate the strain slant to mature, get plump spores, set aside;
[0034] Strain fermentation: Add the epigenetic modification regulator TSA to the fungal ...
Example Embodiment
[0039] Example 3:
[0040] A preparation method for isolating and extracting new compounds from A. terreus secondary metabolites, including spore preparation, strain fermentation, secondary metabolite extraction, and separation and purification. The specific steps are:
[0041] Spore preparation: Place the glycerol cryopreservation tube of strain A.terreus OUCMDZ-2739 (containing 20% glycerol and 3.3% seawater in a freshly prepared PDA liquid cryopreservation solution) stored in an ultra-low temperature refrigerator at -80°C in a clean operating table. Resuscitate for 10 minutes. Dip the resuscitated cryopreservation liquid with the inoculating loop after the burning and sterilization, and streak evenly in the freshly prepared PDA slope solid medium, and then place the slope medium in a 28℃ constant temperature incubator for culture 5 Day, cultivate the strain slant to mature, get plump spores, set aside;
[0042] Strain fermentation: Add the epigenetic modification regulator TSA ...
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