Storage tolerance cell cryopreservation liquid

Abstract

The invention discloses a storage tolerance cell cryopreservation liquid which comprises propylene glycol, dextran, potassium chloride, magnesium chloride, disodium hydrogen phosphate, monopotassium phosphate, sodium chloride, vitamin C, cholesterol, sodium bicarbonate, albumin, glucose, creatinine, chloramphenicol, streptomycin, glycerinum, sodium carboxymethylcellulose and sterile injection water. The components of the storage tolerance cell cryopreservation liquid disclosed by the invention are coordinated with one another and act with one another, so that the cryopreservation liquid disclosed by the invention can be easily preserved and has a relatively long shelf life, that is, the shelf life is as long as 3 months or grater, the operation is simple, the cryopreservation liquid does not need to be prepared temporarily on site, the time and the labor are saved, the cryopreservation liquid can be preserved at low temperature directly, no complex cryopreservation procedure is needed, the cell cryopreservation time is greatly shortened, the cryopreservation efficiency is improved, and the cryopreservation liquid is stable and reliable in preservation effect, convenient to transport, convenient to use, simple and easily available in raw material and worthy of market popularization and application.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a storage-stable cell cryopreservation solution. Background technique [0002] Cell cryopreservation is to store cells in a low temperature environment, so that cells can temporarily escape from the growth state and preserve their cell characteristics. The cost of such preservation is low, and the cells can be revived and cultured when needed, and at the same time, the loss of cell varieties caused by cell contamination during culture and preservation is also avoided. The most common method of long-term cryopreservation to maintain cell viability is liquid nitrogen cryopreservation, which can maintain more than 80% cell viability. However, in the transportation and clinical application of cell products, liquid nitrogen is not easy to transport by means of transportation, and there are great limitations in the transfer and transportation of frozen cells in liquid ...

AI Technical Summary

Problems solved by technology

This kind of freezing solution is generally a ready-to-use freezing solution, which needs to be prepared and used immediately, with large batch differences, short storage period, and unstable storage effect