The invention discloses a test strip for detecting creatinine by an electrochemical method. The test strip comprises: a substrate, a working electrode, a reference electrode, a counter electrode, a startup electrode, an insulating layer, a first enzyme carrier layer, a second enzyme carrier layer, a blood filtering membrane, a diffusion layer, a double faced adhesive tape layer, siphon holes and ahydrophilic layer. A formula of a first reaction solution on the first enzyme carrier layer comprises: creatininase, sarcosine oxidase, creatine hydrolase, ascorbic acid oxidase, peroxidase, a dispersing agent, a surfactant and a buffer solution; a formula of a second reaction solution on the second enzyme carrier layer comprises: sarcosine oxidase, creatine hydrolase, ascorbic acid oxidase, peroxidase, a stabilizer, a surfactant and a buffer solution; the detection result of the invention is well matched with a hospital inspection result, and the use is simple.