166 results about "Cholesterol oxidase" patented technology

The present invention relates to an enzyme electrode useful for estimation of cholesterol in aqueous medium, said electrode comprising: i. an electrically conductive base plate, ii. a film of sol gel derived material deposited thereon, said sol gel derived material of step b) being microencapsulated cholesterol oxidase with an electron mediator. The present invention also relates to a process for the preparation of an enzyme electrode by coating an immobilized cholesterol oxidase (ChOx) and mediator on a silicate sol gel by microencapsulation.

The present invention provides a kind of efficient enzyme combining stabilizer with high stabilizing effect on enzymes for clinical diagnosis reagent. The enzyme combining stabilizer consists of bovine serum albumin, EGTA, 1, 2-dithio threitol, potassium gluconate, sodium chloride, proclin300, magnesium acetate, etc. It has powerful stabilizing effect on lactate dehydrogenase, sarcosie oxidase, urease, creatinine enzyme, creatine hydrolase, etc.

Disclosed is an enzyme electrode having an oxidoreductase (for instance, glucose oxidase, cholesterol oxidase, fructosylamine oxidase and glucose dehydrogenase) and an electron-transfer protein (for instance, cytochrome C, cytochrome b562 and cytochrome c551), as well as a sensor utilizing the enzyme electrode as working electrode. The enzyme electrode of the invention can provide high response current values.

The invention discloses a kit for detecting high density lipoprotein cholesterol. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution I, 0.1-10 mmol / L polyanionic compound, 0.1-100 mmol / L bivalent cation, 0.1-10 mmol / L developer, 1-100 g / L surfactant I, 1-100 g / L surfactant II and 1-50 g / L preservative agent; and the reagent R2 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution II, 0.1-10 mmol / L 4-aminoantipyrine, 1-100 g / L surfactant III, 0.1-10 KU / L cholesterase, 0.1-10 KU / L cholesterol oxidase, 0.1-10 KU / L peroxidase and 1-50 g / L preservative agent. According to the invention, improvement is made on the basis of a selective inhibition method; the preferable compound surfactant pairs are added to inhibit and eliminate lipemia interference in the first reaction step, so that lipemia samples can be directly detected by a full-automatic biochemical analysis instrument without being pretreated; and the kit is simple to operate, improves the detection efficiency and lowers the detection cost.

The invention provides a 3-ketosteroid-delta 1-dehydrogenase gene, cholesterol oxidase coded by the same, an expression vector containing a gene sequence of the 3-sterone-delta 1-dehydrogenase gene, a gene engineering recombinant strain containing the expression vector and a method for preparing 3-sterone-delta 1-dehydrogenase. The 3-sterone-delta 1-dehydrogenase provided by the invention is new 3-sterone-delta 1-dehydrogenase. The recombinant strain provided by the invention can be used for transforming 3-keto steroids, wherein the optimized recombinant strain can also be used for transforming androstane-4-alkenyl-3,17-diketone into boldenone, the expressed target protein is a soluble protein, the bottleneck of membrane protein in the traditional industrial production is broken through, and the recombinant strain has great significance in the industrial production of KSDD (3-ketosteroid -delta 1-dehydrogenase). Steroids are produced through microbial transformation, the production efficiency and the product quality of a steroid medicine production system are improved, and the production cost is reduced.

The invention relates to an in-vitro diagnostic reagent for a homogeneous method of low-density lipoprotein cholesterol (LDL-C) of serum, wherein the in-vitro diagnostic reagent is capable of being widely applied to the technical field of medicine and biochemistry and is characterized in that the in-vitro diagnosis is carried out by means of a method comprising the following steps of: step one, selectively cracking chyle particles (CM), very low density lipoprotein cholesterol (VLDL-C) and high density lipoprotein cholesterol (HDL-C) within the serum by using a group of surfactant comprising trimethyl-beta-cyclodextrin, ethylene oxide octadecyl amine, poloxamer F88 and Brij-58, then generating hydrogen peroxide (H2O2) during the catalytic reaction of cholesterol esterase (COE) and cholesterol oxidase (COD), and then discomposing the H2O2 by means of a chemiluminescence clearing system of hydrogen peroxide, wherein the LDL-C particles within the serum are still kept perfectly at the moment; step two, reacting the LDL-C by catalyzing with the COE and the COD under the effect of TritonX-100 so as to generate H202, then promoting a chemiluminescence quantitative system to produce chemiluminescence by catalyzing the H2O2 with POD, and quantitating the LDL-C after measuring luminous intensity. The measuring reagent provided by the invention has the advantages that the sensitivity is high, the capacity of resisting disturbance is strong, the purpose for detecting the LDL-C of serum in batch is realized on a microporous plate chemiluminescence apparatus by measuring chemiluminescence intensity, and the reagent is suitable for the application in clinical laboratory.

Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.

The invention discloses a microelectrode array which contains microelectrodes formed by micro holes of a cell size. Single cells are positioned directly on the microelectrodes containing the micro holes of the cell size, activation cholesterol in cell membranes reacts with cholesterol oxidase in a solution to produce hydrogen peroxide on the electrode surface to cause measurable luminol electroluminescence, and the luminous intensity is related to the activation cholesterol amount in the cell membrane. By connecting with a multichannel converter and a voltage generator, pulse triangle wave voltages are successively applied to the microelectrodes in the array, when a voltage is applied to the surface of a microelectrode, the cell or the microelectrode may shine, and a photomultiplier (PMT) is used for detection of luminol chemiluminescence intensity to obtain the activation cholesterol amount in the membranes. In the microelectrode array, the single cells are selected by the voltages to detect, the microelectrode array facilitates detection automation and analysis of flux. The microelectrode array can realize the rapid detection of the activation cholesterol content in the cell membranes.

The invention belongs to the technical field of electrochemical biosensors and particularly relates to an enzyme biosensor for detecting cholesterol as well as a preparation method and an application of the biosensor. The enzyme biosensor adopts a classical three-electrode system, wherein a specific matter recognizing enzyme membrane is solidified on a working electrode and mainly formed by mixing graphene, thionine, cholesterol oxidase, horse radish peroxidase and chitosan. The preparation method of the enzyme biosensor comprises the following steps: firstly uniformly mixing a thionine solution, a chitosan solution of the graphene, a horse radish peroxidase solution and a cholesterol oxidase solution, dripping onto the processed working electrode, forming the three-electrode system together with a reference electrode and a counter electrode to obtain the enzyme biosensor. The enzyme biosensor disclosed by the invention has the advantages of economical efficiency, simplicity, rapidness, sensitivity and the like, is simple in preparation and can be used for the quantitative determination of the cholesterol.

This invention discloses an associating determination and reagent about high and low-density lipoprotein cholesterol. It utilizes affinity difference between lipoprotein and surface activator, adds agent one and agent two, and with the action of the assembling ionic surface activator, the CM, VLDL and LDL-C or HDL-C in the serum form the soluble complex, the extricate cholesterol generates H#-[2]O#-[2] with the catalytic reaction of cholesterol esterase (CHER) and cholesterol oxidase (CHO), and with the action of peroxydase (POD), the H#-[2]O#-[2] is cleared, and adds agent three, as with the action of a specific selective surface activator, only HDL-C or LDL-C granule is soluble, so with action of Trinder one can determine the content of HDL-C and LDL-C.

The invention discloses a reagent, a method and a kit for measuring small-and-dense lipoprotein. The reagent comprises first agents and second agents, the first agents include 1-500 mg / L reagent A, 10-300 U / mL sodium heparin, 0.1-90 mmol / L divalent metal ion, 0.5-2 KU / L cholesterol esterase, 1-3.5 KU / L cholesterol oxidase, 100-300 KU / L catalase, 0.1-10 mmol / L 4-amino antipyrine, 0.05-6% KU / L surfactant A, and the second reagents include 0.2-10 KU / L peroxidase, 0.3-20 mmol / L Trinder's chromogen compound, 0.01-0.3% sodium azide and 0.05-12% surfactant B. The reagent can be used for specifically detecting sdLDL. By using the method, sdLDL can be detected specifically. The kit is simple and convenient to operate.

The invention discloses a detection kit for LDL (low-density lipoprotein) cholesterol and a use method of the detection kit, relates to the field of biochemical detection and aims to provide a detection kit, having high stability, accuracy and precision as well as low toxicity, for LDL cholesterol and a use method of the detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from a surfactant, polyethylene glycol, SDS (sodium dodecyl sulfate), Emulgen A9 series, CHOD (cholesterol oxidase), CHER (cholesterol esterase), ascorbic acid oxidase, CAT (catalase), 4-AAP (4-aminoantipyrine) and the like; the reagent 2 is prepared from octylphenyl polyethylene glycol, cholate, a compound stabilizer, bovine serum albumin, POD (peroxidase), TOPS (sodium 3-(N-ethyl-3-methylanilino)propanesulfonate) and the like. The proclin series added to the kit is a novel biological preservative, has good compatibility with various enzymes, better stability and low toxicity, and the stability of the reagents is maintained.

The invention provides a small and dense low-density lipoprotein cholesterin (sdLDL-C) detection kit which comprises double liquid reagents, including a reagent R1 and a reagent R2, wherein the reagent R1 comprises an MOPS buffer liquid (the pH value is 7.0), cholesterol esterase, cholesterol oxidase, phospholipase, catalase, polyoxyethylene alkyl phenyl ether (JK-14) as a surfactant A, polyoxyethylene benzyl styrene ether (A3PK) as a protecting agent, and N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3-methylaniline sodium salt (TOOS); the reagent R2 comprises an MOPS buffer liquid (the pH value is 7.0), peroxidase, polyethylene glycol octylphenol ether (Triton X-100) as a surfactant, 4-amino antipyrine (4-AA), and sodium azide. The method provided by the invention is high in sensitivity, high in specificity, simple in reagent preparation, free of sample pretreatment, and worthy of popularization and application.

The invention provides a reagent for directly and quantitatively determining low-density lipoprotein cholesterol (LDL-C) in human serum in a homogeneous system by an enzymatic method. The reagent is suitable for automatically and quantitatively determining the LDL-C by an automatic biochemical analyzer. The reagent consists of a solution type reagent 1 and a solution type reagent 2 which are placed separately, wherein the reagent 1 contains cholesterol esterase, cholesterol oxidase, 4-amino-antipyrine, catalase, surfactant, a buffering agent and a stabilizing agent; and the reagent 2 comprises peroxidase, a color-developing agent, the other surfactant, a stabilizing agent and a buffering agent. The invention also provides a method for directly determining the content of the LEDL-C in the homogeneous system without pre-treating a serum sample, and a kit which specifically uses the method in a clinical laboratory and in which the reagent 1 and the reagent 2 are accommodated.

The invention relates to multi parameter whole blood integrated test bar. It combines the testing instrument and could measure the blood sugar, uric acid or cholesterol parameters in time-sharing or multiple steps. The invention uses the base compounded by polymer as main body, and conductive silver layer, conductive carbon layer, insulating layer, undrying layer and covering film layer are over the base. The invention set glucose oxidase, urate oxidase or cholesterol oxidase are set on the same base. The test bar side would take sample, and the reacting pools are separated, and would not disturb with each other while taking electrochemical reaction.

The invention discloses a total cholesterol detection reagent and total cholesterol detection paper. The total cholesterol detection reagent comprises 6-9 KU / L cholesterol esterase, 3-5 KU / L cholesterol oxidase, 15-20 KU / L horseradish peroxidase, 3-5 KU / L ascorbic acid oxidase, 0.20-0.35 g / L 4-ampyrone, and 0.20-0.30 g / L color-showing matter. The detection reagent contains a plurality of specific enzymes in a specific ratio, and can achieve the purposes of rapid detection and relatively accurate detection. The detection paper comprises a reaction base layer, a reaction layer, a blood filtering layer and a hydrophilic layer, which are overlapped to form a siphon system, thereby further ensuring rapid detection and relatively accurate detection.

The invention relates to a process for degrading cholesterol in yolk, which is a process method for degrading the cholesterol in the yolk by using cholesterol oxidase with the aid of ultrasonic waves. The process comprises the following steps of: performing ultrasonic treatment on the yolk by using the ultrasonic waves, adding the cholesterol oxidase and putting on a rocking bed for degrading, wherein preferably, the power of the ultrasonic waves is 200W and the frequency is 50MHz, intermittent ultrasonic treatment is performed, the treatment volume of the ultrasonic treatment is 20 to 30ml, 0.586 to 0.726U of cholesterol oxidase is used for each gram of yolk, and the degradation time is 9 to 11 hours. The process for degrading the cholesterol in the yolk has a smart design, high selectivity and efficiency of degrading the cholesterol, and low cost, is simply, conveniently and quickly operated, pollution-free and suitable for large-scale industrial production, and particularly ensures that the degradation product is single, and the loss of nutrient substances such as phospholipids and the like is low.

The invention discloses a method for determining cholesterol by using flow injection chemiluminescence with nano-copper oxide as a catalyst. The method comprises the following steps: mixing cholesterol, cholesterol oxidase and phosphatic buffer solution; warm bathing the mixture solution to obtain a reaction solution; after diluting the reaction solution, inputting the diluted reaction solution into a mixer through a peristaltic pump to mix with a nano-copper oxide colloidal solution; then mixing with a luminol solution; entering into a flow cell for reaction; and detecting the generated chemiluminescence intensity by a photomultiplier to determine the cholesterol. By using cholesterol oxidase for catalyzing the oxidation of cholesterol to generate hydrogen peroxide, combining the flow injection technique, the chemiluminescence signal in the determination of cholesterol is enhanced about 600 times. By using cholesterol-cholesterol oxidase-luminol-nano-copper oxide flow injection chemiluminescence to determine the cholesterol content, the linear range is 0.625-12.5 [mu]mol / L. the method can be used for determination of food cholesterol content and determination of the free cholesterol and total cholesterol in serum.

A method for specifically quantifying HDL cholesterol in which cholesterol in lipoproteins other than HDL is erased in the first step, and HDL cholesterol is specifically quantified in the second step, by which accurate values can be obtained even in measurements of abnormal samples such as disorder of lipid metabolism and lipoprotein abnormality, is disclosed. The method for quantifying cholesterol in high density lipoprotein according to the present invention comprises a first step of erasing cholesterol in lipoproteins other than high density lipoprotein by treating a test sample with cholesterol esterase and cholesterol oxidase in the absence of a surfactant which acts on high density lipoprotein and removing generated hydrogen peroxide; and a second step of adding a surfactant which specifically acts on high density lipoprotein to the product of said first step and quantifying hydrogen peroxide generated from cholesterol in high density lipoprotein by actions of cholesterol esterase and cholesterol oxidase. As the cholesterol oxidase used in the first step, one having a molecular weight of not more than 60 kilodaltons is used.

The invention discloses a high-density lipoprotein cholesterol detection kit which consists of a first reagent and a second reagent, wherein the volume of the first reagent is 3 times of that of the second reagent; the kit comprises a surfactant, cholesterol esterase, cholesterol oxidase, peroxidase, absolute ethyl alcohol and a thiophosphoric reagent. In the first reagent, non-high-density lipoprotein cholesterol in a serum sample is in reaction with the cholesterol esterase, the cholesterol oxidase and the peroxidase under the action of the surfactant, thereby being eliminated; in the secondreagent, the rest serum with high-density lipoprotein cholesterol is extracted through the absolute ethyl alcohol, proteins in the serum are denaturated, the solubility is degraded, then precipitateis generated, cholesterol is dissolved in the ethanol, and under the action of the thiophosphoric reagent, the cholesterol is in reaction with the thiophosphoric reagent, and then the content of the cholesterol is detected. Test results show that the kit disclosed by the invention is wide in linear range, high in accuracy and precision and in addition good in interference resistance.

The invention discloses a high-performance small and dense low-density lipoprotein cholesterol detection kit. The kit comprises the following raw materials in parts by weight; a reagent A: 90 to 110 mmol / L of a Good's buffer solution; 1-3 ku / L of cholesterol esterase, 1-2 ku / L of cholesterol oxidase, 0.7-0.9 ku / L of phospholipase, 400-600 ku / L of catalase, and 1-3 mmol / L N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS); and a reagent B: 90-110 mmol / L of the Good's buffer solution, 2-3 ku / L of peroxidase, 3-5 mmol / L of 4-aminoantipyrine and 0.04-0.06% of sodium azide. Theinvention relates to the technical field of lipoprotein cholesterol detection, and discloses the high-performance small and dense low-density lipoprotein cholesterol detection kit. The non-sdLDL-C components are removed through cholesterol lipase, cholesterol oxidase, phospholipase and catalase; the interference of other lipoprotein cholesterol on the detection process is avoided, the capacity ofthe small and dense low-density lipoprotein cholesterol in serum can be directly determined by using a full-automatic biochemical analyzer, and the kit is suitable for the requirements of clinical andlaboratory on the determination of the content of the small and dense low-density lipoprotein cholesterol.

The invention discloses a kit for detecting small and dense low-density lipoprotein cholesterin. The kit is characterized by comprising polyoxyethylene fatty acid ester, cholesterol esterase, cholesterol oxidase, dodecyl polypeptide and triton X-100, wherein the small and dense low-density lipoprotein cholesterin in a serum sample is protected under the action of the polyoxyethylene fatty acid ester, reaction of the small and dense low-density lipoprotein cholesterin with the cholesterol esterase and the cholesterol oxidase is inhibited to a certain extent, and the other lipoprotein cholesterols react with the cholesterol esterase and the cholesterol oxidase under the action of the dodecyl polypeptide to be eliminated; and the triton X-100 reacts with the remaining small and dense low-density lipoprotein cholesterin to detect the content thereof. The kit can be used for quickly and conveniently detecting sdLDL-C in the serum sample. The reagent formula is simple and easy to prepare and low in cost, large-scale industrial preparation can be carried out, and meanwhile, the kit is capable of efficiently, accurately and specifically detecting the small and dense low-density lipoprotein cholesterin in the sample.

This invention relates to a method of controlling pathogens, by use of oxalate-producing enzyme, alone or in combination with toxic protein may be applied directly to the plant or produced thereon by microorganisms or by genetically modifying the plant to produce the enzyme, and to genes, microorganisms, and plants useful in that method. In one aspect this invention relates to a method of controlling insects, including lepidopterans and boll weevils, by use of oxalate-producing enzyme, alone or in combination with a crystal protein from Bacillus thuringiensis or cholesterol oxidase which may be applied directly to the plant or produced thereon by microorganisms or by genetically modifying the plant to produce the enzyme, and to genes, microorganisms, and plants useful in that method.

The invention provides a cholesterol oxidase gene, cholesterol oxidase coded by the cholesterol oxidase gene, an expression vector containing a gene sequence of the cholesterol oxidase gene, a gene engineering recombinant strain containing the expression vector and a method for preparing the cholesterol oxidase. The cholesterol oxidase recombinant strain provided by the invention can be used for performing 3-site hydroxyl dehydrogenation or sterol metabolism on 3-hydroxyl steroids and can also be used for degrading sterols, so that the problem of low utilization ratio of mycobacteria and rhodococcus for degrading sterols in the sterol medicine industry is solved.

The invention discloses an enzymatic detection kit for small and dense low density lipo-protein cholesterol. The enzymatic detection kit for the small and dense low density lipo-protein cholesterol comprises a reagent R1, a reagent R2 and a calibrator; the reagent R1 consists of the following components at specific concentrations: a buffer solution, cholesterol lipase, cholesterol oxidase, catalase, an anti-human high-density lipo-protein antibody, a surfactant, and a preservative; the reagent R2 consists of the following components at specific concentrations: a buffer solution, a surfactant,4-aminoantipyrine, peroxidase, and a preservative; and the calibrator consists of the following components at specific concentrations: a buffer solution, glycerol, a stabilizer, a small and dense lowdensity lipo-protein cholesterol (sdLDL) antibody, and a preservative. In virtue of addition of the anti-human high-density lipo-protein antibody, the enzymatic detection kit for the small and dense low density lipo-protein cholesterol has greatly improved kit performance, better reagent stability, higher sensitivity, wider detection range and reduced detection cost.

The invention discloses a reagent for lipid typing detection. The reagent is characterized by comprising alkylphenol polyethoxylate, Triton X-100, cholesterol esterase, cholesterol oxidase, peroxidase, phosphate buffer, phenol, 4-aminoantipyrine, and PC300. For a formula of the reagent, lipoprotein can be dissolved and release cholesterol or cholesteryl ester contained internally through additionof alkylphenol polyethoxylate and Triton X-100 in a specific ratio and in a mixing ratio, cholesterol ester can generate cholesterol under the action of cholesterol esterase, cholesterol can generatecholest-4-ene-3-ketone and hydrogen peroxide under the action of cholesterol oxidase, hydrogen peroxide acts with phenol and 4-aminoantipyrine (4-AAP) under the action of peroxidase so as to generatea colored material, and contents of different lipoproteins are calculated by checking changes of absorbance.

The invention discloses a small dense low-density lipoprotein cholesterol kit. The kit consists of two independent reagents which are R1 and R2; the R1 contains a buffer solution, lipoprotein lipase,a nonionic surfactant, serum albumin, heparin sodium, magnesium ions, cholesteryl esterase, cholesterol oxidase, catalase or peroxidase, a Trinder's reactant A, a stabilizing agent and an antiseptic;and the R2 contains the buffer solution, the nonionic surfactant, a Trinder's reactant B, sodium azide and the antiseptic. The reagent R1 can be taken to mix with a specimen to eliminate cholesterol except for SD-LDLC; and reaction can be performed on the SD-LDLC after the reagent R2 is added, the Trinder's reactant B can be stained to determine absorbance, and content can be calculated. The kit is good in stability, high in accuracy, simple and fast in operation, and suitable for automatic biochemical analyzer detection.

The invention provides a lipoprotein cholesterol determination reagent and a kit, belonging to the technical field of biochemical detection. The reagent comprises poly-alpha-olefin, cholesterol oxidase and reaction promoter, and further comprises cholesterol esterase, peroxidase, catalase, catalase inhibitor, surface active agent, emulsifier, polyanion, divalent cation, stabilizer, anti-interference agent, color developing agent and preservative. The reagent and the kit provided by the invention can be used for full-automatic biochemical analyzer detection, are simple and convenient to operate, can improve detection efficiency and accuracy and can reduce detection cost.

The invention relates to a preparation method of a cholesterol oxidase modified hybrid metal organic framework tumor targeting nano preparation, which can effectively solve the problem that the treatment of tumor multidrug resistance is more accurate, safer and more efficient in targeting. The hybrid metal organic framework is a metal organic framework (MOF) with catalase-like activity, cholesterol oxidase is modified on the surface of the metal organic framework (MOF), an anti-tumor drug is physically loaded in the metal organic framework (MOF) to form nanoparticles, and finally, the nanoparticles are wrapped with a gel shell to obtain the pharmaceutical composition with the particle size of 250-300 nm. The preparation method is simple and convenient, the cost is low, and the prepared cholesterol oxidase modified hybrid metal organic framework gel shell pharmaceutical composition can enhance the anti-tumor effect, is an innovation in tumor treatment pharmaceutical preparations, and has huge economic and social benefits.

The invention relates to a high density lipoprotein cholesterol detection kit which is a liquid-type dual-reagent composed of a reagent R1 and a reagent R2. The reagent R1 comprises: 0.5-4.0 mmol / L of a coupling agent (DSBmT), 3500-8500 U / L of a cholesterol oxidase (CHOD), 1000-4000 U / L of a peroxidase (POD), 0.5-1.5 g / L of a surfactant and 50-160 mmol / L of a buffer solution (pH=6.0). The reagent R2 comprises: 1.0-4.5 mmol / L of 4-aminoantipyrine (4-AAP), 700-1300 U / L of a cholesterol esterase, 0.5-1.5 g / L of a surfactant and 50-160 mmol / L of a buffer solution (pH=6.0). The high density lipoprotein cholesterol detection kit is better in specificity, has stronger in anti-interference capability, has anti-interference capabilities of hemoglobin, bilirubin, ascorbic acid and rntralipos and is higher in linearity. The kit is suitable for a full-automatic biochemical analyzer and has a great clinical application value. Stability time of the reagents can reach two years. The kit is reasonable in design, strong in practicality and is suitable for a wide application and popularization.