Kit for detecting small and dense low-density lipoprotein cholesterin

Abstract

The invention discloses a kit for detecting small and dense low-density lipoprotein cholesterin. The kit is characterized by comprising polyoxyethylene fatty acid ester, cholesterol esterase, cholesterol oxidase, dodecyl polypeptide and triton X-100, wherein the small and dense low-density lipoprotein cholesterin in a serum sample is protected under the action of the polyoxyethylene fatty acid ester, reaction of the small and dense low-density lipoprotein cholesterin with the cholesterol esterase and the cholesterol oxidase is inhibited to a certain extent, and the other lipoprotein cholesterols react with the cholesterol esterase and the cholesterol oxidase under the action of the dodecyl polypeptide to be eliminated; and the triton X-100 reacts with the remaining small and dense low-density lipoprotein cholesterin to detect the content thereof. The kit can be used for quickly and conveniently detecting sdLDL-C in the serum sample. The reagent formula is simple and easy to prepare and low in cost, large-scale industrial preparation can be carried out, and meanwhile, the kit is capable of efficiently, accurately and specifically detecting the small and dense low-density lipoprotein cholesterin in the sample.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Lipoproteins in blood can be divided into four categories according to their density: high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL) and chylomicrons (CM). Different types of lipoprotein cholesterol have different abilities to cause atherosclerosis. Usually the most clinically detected components are high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Conventionally speaking, high-density lipoprotein cholesterol has a certain protective effect on arteries. The higher the content, the lower the risk of cardiovascular disease, and the low-density lipoprotein cholesterol can easily cause atherosclerosis and increase the risk of cardiovascular disease. However, recent studies have shown that even...

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Problems solved by technology

However, the steps of electrophoresis determination are cumbersome, and the precision and accuracy need to be improved, so the clinical applicability is not strong; the ultracentrifugation method is the standard method for the determination of lipoproteins, but the detection of sdLDL by this method requires an ultracentrifuge, and the detection is generally The amount is not high, and the requirements for operators are high, so this method has not been well promoted in clinical practice; although the fractional precipitation method makes the operation relatively simple, it still needs a precipitation step. The precision and accuracy of this method are relatively poor and not suitable for clinical application

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