High-performance small and dense low-density lipoprotein cholesterol detection kit

Abstract

The invention discloses a high-performance small and dense low-density lipoprotein cholesterol detection kit. The kit comprises the following raw materials in parts by weight; a reagent A: 90 to 110 mmol / L of a Good's buffer solution; 1-3 ku / L of cholesterol esterase, 1-2 ku / L of cholesterol oxidase, 0.7-0.9 ku / L of phospholipase, 400-600 ku / L of catalase, and 1-3 mmol / L N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt (TOOS); and a reagent B: 90-110 mmol / L of the Good's buffer solution, 2-3 ku / L of peroxidase, 3-5 mmol / L of 4-aminoantipyrine and 0.04-0.06% of sodium azide. Theinvention relates to the technical field of lipoprotein cholesterol detection, and discloses the high-performance small and dense low-density lipoprotein cholesterol detection kit. The non-sdLDL-C components are removed through cholesterol lipase, cholesterol oxidase, phospholipase and catalase; the interference of other lipoprotein cholesterol on the detection process is avoided, the capacity ofthe small and dense low-density lipoprotein cholesterol in serum can be directly determined by using a full-automatic biochemical analyzer, and the kit is suitable for the requirements of clinical andlaboratory on the determination of the content of the small and dense low-density lipoprotein cholesterol.

Description

technical field [0001] The invention relates to the technical field of lipoprotein cholesterol detection, in particular to a high-performance small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Sterols often exist in the form of lipoproteins in the blood, and low-density lipoproteins in plasma are the main carrier for transporting endogenous cholesterol, which are degraded and transformed by binding to low-density lipoprotein receptors on the cell membrane. Low-density lipoprotein cholesterol is composed of LDL-C, which is the main lipoprotein in fasting plasma, accounting for about 2 / 3 of plasma lipoproteins, and is the main vehicle for transporting cholesterol to extrahepatic tissues; LDL-R dysfunction can cause plasma LDL The scavenging ability of -C is reduced, which eventually leads to the formation of atherosclerotic plaque in the arterial intima. Therefore, the content of LDL-C is related to the incidence of cardiovascular...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a high-performance small and dense low-density lipoprotein cholesterol detection kit, which solves the problem of the existing small and dense low-density lipoprotein cholesterol detection kit, directly through the detection of sdLDL-C Carry out coloring to detect its content, and other lipoprotein cholesterol will be partially transformed during the catalytic process of the detection reagent

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