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Reagent, method and kit for measuring small-and-dense lipoprotein

A lipoprotein and reagent technology, applied in the field of biomedicine, can solve the problem of lack of detection methods for small and dense lipoproteins

Active Publication Date: 2016-06-08
上海微鸿企业管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a reagent, method and kit for measuring small and dense lipoproteins in view of the lack of a detection method specifically for small and dense lipoproteins at present

Method used

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  • Reagent, method and kit for measuring small-and-dense lipoprotein
  • Reagent, method and kit for measuring small-and-dense lipoprotein
  • Reagent, method and kit for measuring small-and-dense lipoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] First reagent:

[0081] Add MOPS buffer, magnesium sulfate, sodium chloride, EDTA, BSA, PVS, sodium heparin, 4-aminoantipyrine and EmulgenA-90 into water, stir until completely dissolved, adjust the pH to 6.40-6.70, then add cholesterol esterase, cholesterol oxidase, and catalase at the following concentrations:

[0082]

[0083]

[0084] Among them, the molecular weight of PVS is 175,000.

[0085] Second reagent:

[0086] Add MOPS buffer, TOOS, sodium azide and Pluronic F-88 into water, stir until completely dissolved, adjust the pH to 6.40-6.70, then add peroxidase into the water and mix, and make the above substances reach the following concentrations:

[0087]

[0088] On the Hitachi 7180 automatic biochemical analyzer, 180 μL of the above-mentioned first reagent was reacted with 2.4 μL of clinical human serum samples for 5 minutes, and then 60 μL of the second reagent was added, and the two-point endpoint method was used at the primary / secondary 546nm / 70...

Embodiment 2

[0093] First reagent:

[0094] Add MOPS buffer, 4-aminoantipyrine, PEG6000, sodium heparin, magnesium sulfate, sodium chloride, EDTA, EmulgenA-90, EmulgenB-66 and BSA into water, stir until completely dissolved, and adjust the pH to 6.40-6.70 , then add cholesterol esterase, cholesterol oxidase and catalase, and make the above substances reach the following concentrations:

[0095]

[0096] Second reagent:

[0097]Mix MOPS buffer, TOOS, PluronicF-88 and sodium azide into water, stir until completely dissolved, adjust the pH to 6.40-6.70, then add peroxidase, and make the above substances reach the following concentrations:

[0098]

[0099] On the Hitachi 7180 automatic biochemical analyzer, 180 μL of the above-mentioned first reagent was reacted with 2.4 μL of clinical human serum samples for 5 minutes, and then 60 μL of the second reagent was added, and the two-point endpoint method was used at the primary / secondary 546nm / 700nm wavelength, and the reading point 16-34...

Embodiment 3

[0103] First reagent:

[0104] Add MOPS buffer, 4-aminoantipyrine, dextran sulfate 500, heparin sodium, magnesium sulfate, sodium chloride, EDTA, EmulgenA-90, EmulgenB-66 and BSA into water, stir until completely dissolved, and adjust the pH To 6.40-6.70, then add cholesterol esterase, cholesterol oxidase and catalase, and make the above substances reach the following concentration:

[0105]

[0106] Second reagent:

[0107] Put MOPS buffer, TOOS, PluronicF-88 and sodium azide into water, stir until completely dissolved, adjust the pH to 6.40-6.70, then add peroxidase, and make the above substances reach the following concentrations:

[0108]

[0109] On the Hitachi 7180 automatic biochemical analyzer, 180 μL of the above-mentioned first reagent was reacted with 2.4 μL of clinical human serum samples for 5 minutes, and then 60 μL of the second reagent was added, and the two-point endpoint method was used at the primary / secondary 546nm / 700nm wavelength, and the reading p...

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Abstract

The invention discloses a reagent, a method and a kit for measuring small-and-dense lipoprotein. The reagent comprises first agents and second agents, the first agents include 1-500 mg / L reagent A, 10-300 U / mL sodium heparin, 0.1-90 mmol / L divalent metal ion, 0.5-2 KU / L cholesterol esterase, 1-3.5 KU / L cholesterol oxidase, 100-300 KU / L catalase, 0.1-10 mmol / L 4-amino antipyrine, 0.05-6% KU / L surfactant A, and the second reagents include 0.2-10 KU / L peroxidase, 0.3-20 mmol / L Trinder's chromogen compound, 0.01-0.3% sodium azide and 0.05-12% surfactant B. The reagent can be used for specifically detecting sdLDL. By using the method, sdLDL can be detected specifically. The kit is simple and convenient to operate.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a reagent, method and kit for measuring small and dense lipoproteins. Background technique [0002] Lipoproteins present in the blood are roughly classified into four types according to their specific gravity: high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and chylomicrons (CM). Among them, low-density lipoprotein (LDL) is composed of a series of particles of different sizes, densities and chemical compositions. Generally, LDL with smaller particles and higher density in the LDL subfraction is called small dense Low-Density Lipoprotein (sdLDL for short); LDL with larger particles and lower density is called large and light LDL, or LgLDL for short. ; The subgroup between the two is medium-density LDL. Recent studies have found that compared with common LDL, sdLDL has a stronger ability to induce atherosclerosis, and is recognized as...

Claims

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Application Information

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IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 不公告发明人
Owner 上海微鸿企业管理有限公司
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