The invention belongs to the technical field of medical detection and in particular relates to a rapid detection kit of beta 2-microglobulin and application of the rapid detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is composed of potassium dihydrogen phosphate, dodecyl glucoside, octa(ethyleneglycol)mono(dodecyl)ether, nonaethylene glycol monododecyl ether, calcium chloride, p-hydroxybenzoate and water; the reagent R2 is composed of nonaethylene glycol monododecyl ether, dodecyl glucoside, anti-human beta 2-microglobulin antibody latex, p-hydroxybenzoate and water; the diameter of the anti-human beta 2-microglobulin antibody latex is 130nm to 150nm. The rapid detection kit of the beta 2-microglobulin, provided by the invention, has the advantages of high determination sensitivity, high accuracy of a detection result, good repeatability and high stability and is a relatively ideal rapid detection kit of the beta 2-microglobulin; the kit is easy to popularize and apply.