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Rapid detection kit of beta 2-microglobulin and application of rapid detection kit

A technology for detecting kits and microglobulins, which is applied in the field of medical testing, can solve problems such as poor stability and low detection sensitivity, and achieve the effects of high stability, high detection sensitivity, and good sensitivity

Inactive Publication Date: 2017-05-10
GUANGZHHOU HUAHONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the defects of low detection sensitivity and poor stability of β2-microglobulin detection kits in the prior art, the purpose of the present invention is to provide a rapid detection kit of β2-microglobulin and its application to solve the above-mentioned problems. question

Method used

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  • Rapid detection kit of beta 2-microglobulin and application of rapid detection kit
  • Rapid detection kit of beta 2-microglobulin and application of rapid detection kit
  • Rapid detection kit of beta 2-microglobulin and application of rapid detection kit

Examples

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Effect test

Embodiment 1

[0033] Example 1, a rapid detection kit for β2-microglobulin

[0034] The rapid detection kit of β2-microglobulin includes reagent R1 and reagent R2 with a volume ratio of 4:1

[0035] The reagent R1 consists of the following components and their concentrations: Potassium dihydrogen phosphate 100mmol / L, dodecyl glucoside 40mmol / L, octa(ethylene glycol)-(dodecyl) ether 50mmol / L, mono Dodecyl nonaethylene glycol ether 80mmol / L, calcium chloride 2mmol / L and paraben 1mmol / L, the balance is water; the pH value of the reagent R1 is 7.5.

[0036] The preparation method of reagent R1 is: the above-mentioned potassium dihydrogen phosphate, dodecyl glucoside, octa(ethylene glycol)-(dodecyl) ether, monododecyl nonaethylene glycol ether, calcium chloride and Add appropriate amount of water to dissolve the parabens, and then add water to adjust the pH to 7.5.

[0037] The reagent R2 consists of the following components and their concentrations: monododecyl nonaethylene glycol ether 80mmo...

Embodiment 2

[0041] Example 2, a rapid detection kit for β2-microglobulin

[0042] The rapid detection kit of β2-microglobulin comprises reagent R1 and reagent R2 with a volume ratio of 4:1,

[0043] The reagent R1 is composed of the following components and their concentrations: Potassium dihydrogen phosphate 120mmol / L, dodecyl glucoside 50mmol / L, octa(ethylene glycol)-(dodecyl) ether 80mmol / L, mono Lauryl nonaethylene glycol ether 100mmol / L, calcium chloride 3mmol / L and paraben 2mmol / L, surplus is water; The pH value of described reagent R1 is 7.6; The pH value of described reagent R1 The preparation method is as in Example 1.

[0044] The reagent R2 consists of the following components and their concentrations: monododecyl nonaethylene glycol ether 100mmol / L, dodecyl glucoside 50mmol / L, anti-human β2-microglobulin antibody latex 12g / L and Paraben 2mmol / L, surplus is water; The diameter of described anti-human β2-microglobulin antibody latex is 142nm;

[0045] The preparation method o...

Embodiment 3

[0048] Example 3, a rapid detection kit for β2-microglobulin

[0049] The rapid detection kit of β2-microglobulin includes reagent R1 and reagent R2 with a volume ratio of 4:1

[0050] The reagent R1 is composed of the following components and their concentrations: Potassium dihydrogen phosphate 160mmol / L, dodecyl glucoside 70mmol / L, octa(ethylene glycol)-(dodecyl) ether 100mmol / L, mono Lauryl nonaethylene glycol ether 120mmol / L, calcium chloride 4mmol / L and p-hydroxybenzoic acid ester 3mmol / L, surplus is water; The pH value of described reagent R1 is 7.8; The pH value of described reagent R1 The preparation method is as in Example 1.

[0051] The reagent R2 consists of the following components and their concentrations: monododecyl nonaethylene glycol ether 120mmol / L, dodecyl glucoside 70mmol / L, anti-human β2-microglobulin antibody latex 20g / L and Paraben 3mmol / L, surplus is water; The diameter of described anti-human β2-microglobulin antibody latex is 150nm;

[0052] The p...

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Abstract

The invention belongs to the technical field of medical detection and in particular relates to a rapid detection kit of beta 2-microglobulin and application of the rapid detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is composed of potassium dihydrogen phosphate, dodecyl glucoside, octa(ethyleneglycol)mono(dodecyl)ether, nonaethylene glycol monododecyl ether, calcium chloride, p-hydroxybenzoate and water; the reagent R2 is composed of nonaethylene glycol monododecyl ether, dodecyl glucoside, anti-human beta 2-microglobulin antibody latex, p-hydroxybenzoate and water; the diameter of the anti-human beta 2-microglobulin antibody latex is 130nm to 150nm. The rapid detection kit of the beta 2-microglobulin, provided by the invention, has the advantages of high determination sensitivity, high accuracy of a detection result, good repeatability and high stability and is a relatively ideal rapid detection kit of the beta 2-microglobulin; the kit is easy to popularize and apply.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a rapid detection kit for β2-microglobulin and its application. Background technique [0002] β2-microglobulin (β2-microglobin, β2-MG) is a β light chain of human histocompatibility antigen (HLA), which is synthesized and secreted by lymphocytes and nucleated cells. The molecule of β2-microglobulin contains a pair of disulfide bonds without sugar, and its serum protein electrophoresis is located in the β2 region. Almost all nucleated cells in the human body can synthesize β2-microglobulin, but it is mainly synthesized by lymphocytes. β2-microglobulin widely exists in human serum, cerebrospinal fluid, urine, colostrum, saliva and other body fluids, and the content is small. [0003] Diabetic nephropathy is very common in long-term diabetic patients. Long-term development can seriously damage renal function, seriously affect the quality of life of diabetic pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/6854G01N33/531
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner GUANGZHHOU HUAHONG BIOLOGICAL TECH
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