Rat infrarenal abdominal aortic aneurysm model constructed through retroperitoneal approach and construction method

Abstract

The invention relates to the technical field of biological and medical technologies, in particular to a rat infrarenal abdominal aortic aneurysm model constructed through a retroperitoneal approach and a construction method. The operation is relatively simple, risks caused by the abdominal approach can be avoided, pressurized perfusion is not adopted, the perfusion time is shortened to 20 min, lower limb ischemia does not occur, the tumor formation rate is not affected, damage to blood vessels is extremely small, bleeding can be stopped through local compression after perfusion is finished, and the operation success rate is remarkably increased; The construction method comprises the following steps: S1, performing fasting for 12 hours before an operation of a rat, anesthetizing the rat with pentobarbital sodium according to 40-50mg / kg, taking a right side lying position after satisfaction of anesthesia, conventionally preserving skin, performing disinfecting, taking a connecting line from the lower part of a left side posterior axillary line costal margin to the root of a thigh as an incision with the length of 3-3.5 cm, cutting the skin, subcutaneous tissue and muscles layer by layer to see the retroperitoneal fat tissue, finding a potential gap between the peritoneum and lumbar dorsal muscles along the fat tissue, separating the gap by a cotton swab to reach the aorta abdominalis, and separating the aorta abdominalis from the inferior vena cava.

Description

technical field [0001] The invention relates to the technical field of biology and medical technology, in particular to constructing a rat hyporenal abdominal aortic aneurysm model and a constructing method through a retroperitoneal approach. Background technique [0002] The disadvantages of the traditional intraluminal elastase perfusion combined with extraluminal calcium chloride infiltration to establish a rat abdominal aortic aneurysm model: [0003] 1. The modeling method is transperitoneal approach, which is complicated and difficult to operate, and there is a risk of abdominal infection. During the operation, intestinal traction is required, which is very easy to cause intestinal obstruction and damage internal organs. , Retroperitoneal separation prolongs the operation time and increases the risk of death; [0004] 2. Elastase perfusion needs to use hydraulic pressure or a micropump syringe to maintain a perfusion pressure of 100mmHg or higher. However, in practice...

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Problems solved by technology

[0003] 1. The modeling method is transperitoneal approach, which is complicated and difficult to operate, and there is a risk of abdominal infection. During the operation, intestinal traction is required, which is very easy to cause intestinal obstruction and damage internal organs. , Retroperitoneal separation prolongs the operation time and increases the risk of death;

[0004] 2. Elastase perfusion needs to use hydraulic pressure or a micropump syringe to maintain a perfusion pressure of 100mmHg or higher. However, in practice, the same pressure cannot be accurately achieved and maintained. Excessive pressure can cause elastase to enter the circulation and cause the death of rats;

[0005] 3. The perfusion time is long, which takes 30 minutes to 2 hours. If the abdominal aorta is blocked for too long, it is easy to form a thrombus, and the blood concentrates above the blocking site for a long time, which will easily lead to cerebral edema and lower limb ischemia, and the mortality rate is high;

[0006] 4. Ligation and puncture of the iliac artery or femoral artery is required after the end of perfusion, which is prone to unilateral limb ischemic necrosis and high disability rate

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