Method of inhibiting pancreatic cancer development in mice by apolipoprotein AI mimetic peptide L-4F

Abstract

The invention belongs to the technical field of pancreatic cancer research, and in particular relates to a method of inhibiting pancreatic cancer development in mice by apolipoprotein AI mimetic peptide L-4F. Aiming at the problem that the research method for inhibiting the development of pancreatic cancer has high cost and no obvious effect, the following scheme is proposed, including the following steps: establishing a mouse model: C57BL / 6 mice are subjected to fasting for 24 hours before operation and can drink water; 2% sodium pentobarbital (1.5 mL / kg body weight) is injected intraperitoneally to anesthetize animals in each group; for all animals in each group, sodium pentobarbital is injected into the abdomen through a 1.5 cm median incision of the upper abdomen; after pancreas of thepancreatic cancer model group is exposed, apolipoprotein AI mimetic peptide L-4F suspension is injected into the tail of pancreas through a 1mL syringe and the mice are raised in common environment;in 3-5 months, the mental state, body weight and ascites of mice are observed at any time. According to the invention, the mouse model is more stable in establishment, the research cost is saved, theobservation is more intuitive, the multi-parameter experiment is carried out, and the accuracy of the experimental research is improved.

Description

technical field [0001] The invention relates to the technical field of pancreatic cancer research, in particular to a method for inhibiting the development of mouse pancreatic cancer by apolipoprotein AI mimetic peptide L-4F. Background technique [0002] The clinical manifestations of pancreatic cancer depend on the location of the cancer, the course of the disease, the presence or absence of metastasis, and the involvement of adjacent organs. Its clinical features are the short course of the disease, rapid development and rapid deterioration of the disease. The most common symptoms are epigastric fullness, discomfort and pain. Although there is conscious pain, not all patients have tenderness. If there is tenderness, it is consistent with the site of conscious pain. [0003] In existing scientific research, it is often necessary to inhibit the stemness characteristics of pancreatic cancer stem cells, but the existing methods are not ideal in terms of operational convenie...

AI Technical Summary

Problems solved by technology

A method of miR-200a inhibiting the stemness characteristics of pancreatic cancer stem cells in the above-mentioned patent has the following disadvantages: this method is not convenient for the research of pancreatic cancer, and the conventional research of pancreatic cancer mostly uses the method of white mouse experiment, and this method cannot flexibly observe pancreatic cancer. cancer development

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