VX2 rabbit liver transplantation tumor model

Abstract

The invention discloses a VX2 rabbit liver transplantation tumor model. A construction method of the VX2 rabbit liver transplantation tumor model comprises the steps as follows: firstly, an anesthetic for intramuscular injection is prepared, the concentration of the anesthetic is 3% pentobarbital sodium, and the dosage is 1 mL / kg; then the VX2 tumor block for intramuscular injection is processed into a tumor block with the size of 1*1*1 mm<3>, the tumor block is inoculated into the body of a young rabbit in the anesthetic state for tumor formation, and the tumor formation parts are rabbit thigh muscle parts on the two sides of the young rabbit; and finally, separated VX2 tumor tissue is rapidly stored in PBS (Phosphate Buffer Solution) at 4 DEG C, muscular tissues around the tumor are removed by using a cell operation table, the tumor block is cut into 1*1*1 mm<3>, and the tumor block is transplanted into the liver of a healthy adult rabbit for planting. The VX2 rabbit liver transplantation tumor model is high in tumor formation rate, a formed tumor is a single tumor generally, the probability of abdominal cavity implantation metastasis and abdominal cavity infection is low, and follow-up experimental research is facilitated.

Description

technical field [0001] The invention relates to a VX2 rabbit liver transplantation tumor model, which belongs to the technical field of biomedicine. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the most common malignant tumors. Its global incidence rate ranks fifth among men, its cancer-related mortality rate ranks second, and its incidence among women ranks seventh. Ranked sixth. About 85% of them occur in developing countries, especially in areas where hepatitis B virus (hepatic B virus, HBV) is prevalent (Southeast Asia and Africa), where the incidence of liver cancer in China accounts for 46.6% of the global liver cancer incidence. There are usually no obvious symptoms in the early stage of liver cancer. Except for a small number of early liver cancer patients who can receive radical treatment, the vast majority of liver cancer patients are in the middle and late stage when they are discovered, and have lost the opport...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: the rabbit VX2 liver tumor model in the prior art has many complications, low tumor formation rate, poor single tumor formation effect, prone to abdominal dissemination, and time-consuming operation of the tumor seeding method, etc.