Corneal neovascularization animal model and construction method thereof

Abstract

The invention provides a corneal neovascularization animal model and a construction method thereof, and the animal model is constructed by making a sneak tunnel right in front of the corneal limbus at an incision in the center of the cornea of a mouse and implanting filter paper containing vascular endothelial growth factors into the top end of the tunnel, thereby obtaining the corneal neovascularization animal model. Before the central incision of the cornea of the mouse, the pentobarbital sodium solution is injected into the abdominal cavity of the mouse, and the Elkheline eye drops anesthetize the surfaces of the two eyes of the mouse; the filter paper is thin, short in manufacturing time, easy to implant in the operation process and high in molding success rate, so that the repeatability of molding is improved, and the cost investment is greatly reduced; the filter paper is combined with the vascular growth factor to be implanted into the mouse cornea interlayer to induce the mouse cornea neovascularization model, and the mouse cornea neovascularization model can be used for the mouse cornea, has the characteristics of short modeling time, low cost, small trauma and high repeatability, and provides a stable and reliable model system for researching the cornea neovascularization inhibitor.

Description

technical field [0001] The invention belongs to the technical field of animal models, and in particular relates to an animal model of corneal neovascularization and a construction method thereof. Background technique [0002] The animal model of corneal neovascularization is an important means to study the regulation mechanism of corneal neovascularization and to explore and evaluate its prevention and treatment methods. Through physical, chemical, biological, surgical and other means of modeling, corneal neovascularization is artificially induced to produce models similar to human diseases. At present, the main methods for inducing corneal neovascularization models are: (1) Corneal pocket method: The corneal pocket method creates a pocket between the corneal layers, puts the inducer into the pocket, and releases the inducer continuously to induce the An animal model of corneal neovascularization, the model has the advantages of less trauma and easy quantitative analysis af...

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Problems solved by technology

(2) Chemical method: Alkaline chemicals such as sodium hydroxide, silver nitrate, potassium nitrate, etc. are used. The effect of chemical reagents in the cornea lasts for a long time, and may work together with inhibitors on corneal neovascularization, interfering with experimental results

(3) Physical methods: including thermal cautery and corneal suture, this type of modeling method is difficult, traumatic, and inefficient, causing a certain degree of damage to the cornea and interfering with the test results

The trauma of corneal transplantation is large, and the postoperative recovery time is long, which affects and interferes with the experimental observation

[0003] The corneal pouch method usually uses an inducer to implant between the corneal stroma layers. In the past, a gelatin sponge was used to dip 500ng of basic fibroblast growth factor (bFGF), and then wrapped with 2% agarose, and the dried The pellets were implanted into the corneal stroma of rabbits to make corneal neovascularization models. On the one hand, the gelatin sponge-bFGF-agarose sustained-release pellets prepared by this method were relatively large, which was not suitable for mouse corneal pouch implantation, and the sustained-release pellets were prepared. Pills take 4-5 days, which takes a long time and increases the scientific research cycle

On the other hand, gelatin sponge has been used in clinical surgery for a long time to stop bleeding and promote wound healing, but it still has some stimulating factors for corneal neovascularization, which interferes with the experimental results

Therefore, there are disadvantages such as long modeling time, high cost, and difficulty in modeling, and it is easy to fail in modeling, which increases the investment of scientific research funds

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