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244 results about "Mus spretus" patented technology

The Algerian mouse, or western Mediterranean mouse, (Mus spretus) is a wild species of mouse closely related to the house mouse, native to open habitats around the western Mediterranean.

Preparation method and application of humanized gene modification animal model

The invention relates to a humanized gene genetically modified non-human animal, particularly a genetically modified rodent, especially a genetically modified mouse, and in particular relates to a construction method of a humanized PD-L1 gene animal model and application of the model in the biomedicine field.
Owner:BIOCYTOGEN JIANGSU CO LTD +1

Preparation method and application of humanized SIRPA genetically modified animal model

The invention discloses a sgRNA capable of specifically targeting Sirpa gene, a targeting vector, and a SIRPA gene humanized animal model. The invention also discloses a preparation method of an sgRNAvector of the humanized animal model, a preparation method of the humanized animal model and related application. The invention also relates to humanized genetically-engineered non-human animals, inparticular relates to genetically engineered rodents, in particular relates to genetically engineered mice, and in particular relates to a method for constructing the humanized SIRPA gene animal modeland use of the humanized SIRPA gene animal model in the field of biomedicine.
Owner:BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD +1

Preparation method and application of humanized gene modification animal model

The invention relates to a humanized gene genetically modified non-human animal, particularly a genetically modified rodent, especially a genetically modified mouse, and in particular relates to a construction method of a humanized CTLA-4 gene animal model and application of the model in the biomedicine field.
Owner:BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD

Preparation method and applications of humanized gene modified animal models

The invention relates to humanized gene modified non-human animals, especially genetically modified rodents, and especially genetically modified mice, and more specifically relates to a construction method of humanized CD137 gene animal models, and applications of the humanized CD137 gene animal models in biomedicine field.
Owner:BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD +1

Preparation method and application of humanized gene modification animal model

The invention relates to humanized gene modification of non-human animals, especially gene modification of rodents, and particularly gene modification of mice, and specifically relates to an establishment method of a humanized CD27 gene animal model and application thereof in the field of biomedicine.
Owner:BIOCYTOGEN JIANGSU CO LTD +1

Preparation method and application of humanized gene modified animal model

The invention relates to a gene modified non-human animal of a humanized gene, especially a gene modified rodent animal and in especially a gene modified mouse, and in particular relates to a construction method of the humanized BTLA gene animal model, a subsequently generated animal and an application of the animal model in the field of bio-medicine.
Owner:BIOCYTOGEN JIANGSU CO LTD +1

Construction method of mouse model for conditional overexpression of HPV E6 gene at ROSA26 site

The invention provides a construction method of a mouse model for conditional overexpression of an HPV E6 gene at an ROSA26 site, which comprises the following steps: designing and obtaining sgRNA according to the sequence of the HPV E6 gene, and preparing a Cas9 / sgRNA mixture; constructing a targeting vector, carrying out microinjection and obtaining an F0-generation mouse; and obtaining an F1-generation mouse. The method has the advantages of high editing efficiency and targeting ability, easy targeting and more accurate and efficient experimental operation.
Owner:SHANGHAI TONEKER BIOTECH

Immunodeficient mice, manufacturing method thereof and application

The invention belongs to technical field of animal genetic engineering, and specifically relates to immunodeficient mice, a manufacturing method thereof and applications. The manufacturing method comprises using NOD-Scid IL2rg- / - immunodeficient mice (NSI mice), and deleting Foxnl gene or Fah gene. Through deleting the Fah gene on the NSI mice, NSIF mice are obtained. The NSIF mice can be used to efficiently establish a novel humanized mouse model, used for liver physiological and pathological research. Through deleting the Foxnl gene on the NSI mice, the NSIF mice are obtained. The NSIF mice have no body hair, and immune system is further defected. The NSIF mice provide convenience for establishment, monitoring, and measurement of solid tumor.
Owner:湖南昭泰生物医药有限公司

The Construction method and application of type ii animal model of mucopolysaccharide storage disease

This invention provides a method for constructing type ii animal model of mucopolysaccharide storage disease, which comprises synthesizing the oligonucleotide strand and annealing annealing the pUC57 T7 SgRNA with it, then connect to gain a sgRNA expression vector according to the mouse iDS gene design target; to obtain a mouse fertilized egg; mix the transferred Cas9mRNA and sgRNA to obtain an RNA mixture, microinject the RNA mixture to the cytoplasm of that fertilized egg, then implant the it into the female mice to get a F0 generation mice; extract the genomic DNA of them and use them as a template to carry out PCR reaction. The product was digested and the mutant ones were selected to determine Founder Mice; Founder Mice and those wild male mice were mated to obtain F1 mice. The invention also provides an animal model constructed by this method and the usage of the model in the study of mucopolysaccharide sequestration type ii.
Owner:SHAANXI HUIKANG BIO TECH CO LTD

Construction method of mouse with Foxp3 gene knocked out under conditions

The invention provides a construction method of a mouse with Foxp3 gene knocked out under conditions. The invention belongs to the technical field of gene engineering. The method comprises the following steps of: respectively carrying out in vitro transcription on EGE-ZXC-007-sgRNA1 and EGE-ZXC-007-sgRNA9 to obtain RNA1 and RNA9, introducing RNA1 and RNA9 and a targeting vector into fertilized eggs of mice to obtain an F0-generation mouse, and carrying out flox genotype detection to obtain an F0-generation positive mouse; hybridizing the F0-generation positive mouse with a wild mouse to obtainan F1-generation mouse, and detecting the flox genotype of the F1-generation mouse to obtain an F1-generation positive mouse; hybridizing the F1-generation positive mouse with a tissue-specific Cre mouse to obtain a floxed heterozygote mouse; and hybridizing the floxed heterozygote mouse with a Cre-deeter mouse to obtain the mouse with the Foxp3 gene knocked out. By adopting the method provided by the invention, the Foxp3 gene in the mouse can be knocked out in a specific tissue.
Owner:THE AFFILIATED HOSPITAL OF QINGDAO UNIV

Construction method for mouse model realizing conditional site-specific double-overexpression of HPV E6/E7 genes

The invention provides a construction method of a mouse model realizing conditional site-specific double-overexpression of HPV E6 / E7 genes. The construction method comprises the following steps: 1, acquiring mice: (1) respectively designing and acquiring sgRNAs; (2) respectively preparing Cas9 / sgRNA mixtures; (3) constructing a targeting vector; (4) carrying out microinjection and acquiring F0-generation mice, including F0-generation mice with HPV E6 knocked in at the specific ROSA26 site and F0-generation mice with HPVE7 knocked in at the specific H11 site; (5) acquiring F1-generation mice; and (6) acquiring F2-generation positive homozygote mice; and 2, acquiring conditional site-specific HPV E6 / E7 double-knock-in model mice: hybridizing the two F2-generation positive homozygote mice toobtain the conditional site-specific HPV E6 / E7 double-knock-in model mice. According to the method provided by the invention, the animal model which can be stably inherited and accurately regulated and controlled can be obtained.
Owner:SHANGHAI TONEKER BIOTECH

Construction method and application of HBeAg transgenic mouse model

InactiveCN108424930AStable expressionDoes not affect normal biological functionCompounds screening/testingHydrolasesTreatment effectChronic hepatitis
The invention provides a construction method and an application of an HBeAg transgenic mouse model. The method comprises the following steps: CRISPR / Cas9 technology is adopted, an HBeAg gene is inserted into a pliver-HBeAg expression cassette at the Rosa26 gene locus in a fixed-point manner through homologous recombination, and a recombinant R26-e(Alb-HBeAg)1 targeting vector is constructed with an In-Fusion cloning method and contains a 3.3kb 5' homologous arm, pliver-HBeAg and a 3.3kb 3' homologous arm; Cas9mRNA, gRNA and the recombinant R26-e(Alb-HBeAg)1 targeting vector are micro-injectedinto a zygote of a C57BL / 6J mouse, then the zygote is transplanted into the uterus of a C57BL / 6J female mouse, and an HBeAg transgenic mouse is obtained through propagation and purification step by step. The transgenic mouse can specifically express HBeAg in liver, thereby being capable of applying to experimental animal models for researching HBeAg infection mechanism and evaluating treatment effects of therapeutic drugs and vaccines for chronic hepatitis B.
Owner:XI AN JIAOTONG UNIV

Evaluation/screening method for diseases associated with D-amino acid utilizing Dao1-/-mouse

Disclosed is an evaluation method which can select a Dao- / - homozygote rapidly among many animals produced by the mating experiment between a DAO oxygen-defect mouse and another disease model mouse to quantify a D-amino acid contained in many samples rapidly. Specifically disclosed is a method for evaluating the influence of test conditions on a mouse biological tissue or a cultured tissue cell derived from the biological tissue. The method comprises the steps of: providing a Dao1- / - mouse or the like; exposing a biological tissue from the Dao1- / - mouse or the like, or the like, to the test conditions; and analyzing the influence of the exposure of the biological tissue from the Dao1- / - mouse or the like, or the like, to the test conditions.
Owner:KYUSHU UNIV +1

PirB gene-knock-in mouse animal model and construction method thereof

The invention discloses a PirB gene-knock-in mouse animal model, which comprises gRNA1 and gRNA2 for determining specific target sites to be knocked in by PirB gene, wherein the PirB gene is knocked into an intron 1 of ROSA26 gene of a C57BL / 6J mouse; the gene sequence of the gRNA1 is shown in SEQ ID NO.1; and the gene sequence of the gRNA2 is shown in SEQ ID NO.2. The invention further specifically discloses a construction method of the mouse animal model, the mouse animal model provides a good foundation for research on PirB gene function and in-vivo verification. Hybridization of PirB-knock-in animal model with different types of Cre mice can be used to study the functions of PirB in different organs or different cell types or different disease models.
Owner:XIAN MEDICAL UNIV

Embryonically modified animal and method of constructing the same

The present invention relates to a method for efficiently producing a reproducible animal using totipotent cells wherein mitochondrial DNAs (e.g., wild-type DNAs) adapted to nuclear DNAs have been introduced into or substituted with mitochondrial DNAs, and the present invention also relates to an animal obtained by such production method. When the totipotent cells are ES cells derived from an inbred mouse, the tetraploid rescue method is preferably used. In the production of chimeric animals, mitochondrial DNAs of totipotent cells derived from an animal to be used is substituted with wild-type mitochondrial DNAs by the back-crossing method, the nuclear replacement method, or the like, and the cells are injected into a tetraploid fertilized egg, so that a reproducible inbred chimeric animal is produced while avoiding death of the obtained inbred chimeric animal from respiratory disturbances and the like immediately after birth. The thus obtained reproducible chimeric animal can be used for gene function analyses, animal experiments, and the like without carrying out complicated manipulations for generating inbred animals.
Owner:NAGAO YASUMITSU +3

Method for efficiently establishing pure line gene knock-out mouse model

InactiveCN1580251AImprove the efficiency of culling miceOther foreign material introduction processesFermentationMammalBlastosphere
The invention provides a kind of transgenic inhuman mammal by producing klone gene without mouse model. Roll outside self murder gene into the mammal. The expression box contains promoter, self murder gene relation with promoter and termination codon. The natural masculine gene will express in testes organize after transgenic animal builds system. The transgenic animal can provide blastosphere for eliminating gene technic to improve the efficiency of the technic and to solve the problem that mouse genetic background is impure.
Owner:SHANGHAI BIOMODEL ORGANISM SCI & TECH DEV +4

Construction method of mouse model with conditional over-expression of HPV E7 gene at H11 locus

PendingCN110923265ASolve efficiency problemsSolve the problem of \"uncertainty of integration site\"Stable introduction of DNAViruses/bacteriophagesImmune escapeNude mouse
The invention provides a construction method of a mouse model with conditional over-expression of HPV E7 gene at H11 locus. The construction method of the mouse model with the conditional over-expression of the HPV E7 gene at the H11 locus comprises the following steps: designing and obtaining sgRNA; (2) preparing a Cas9 / sgRNA mixture; (3) constructing a targeting vector; (4) co-injecting the targeting vector and the Cas9 / sgRNA mixture into fertilized eggs of a mouse so as to subsequently obtain F0 generation mice; and (5) obtaining F1 generation mice. The construction method of the mouse model with the conditional over-expression of the HPV E7 gene at the H11 locus solves the problem that "nude mice are not suitable for studying role of HPV E7 in HPV E7-mediated processes of tumor microenvironment immune regulation, immune escape and the like for the nude mice lack competent immune systems" in the prior art. The mouse model provided by the invention has a competent immune system, so that the mouse model is suitable for all researches on interaction between the HPV E7 and the immune system.
Owner:SHANGHAI TONEKER BIOTECH

Il21 gene knockout mouse model as well as construction method and application thereof

The invention discloses an Il21 gene knockout mouse model and a construction method and an application thereof. The construction method comprises the following steps that S1, based on the CRISPR / Cas9 technology, a knockout area of an Il21 gene is determined, specific target sites sgRNA1 and sgRNA2 are designed according to the determined knockout area, and gene sequences of the gRNA1 and the gRNA2 are shown in SEQ ID NO.1 and SEQ ID NO.2 respectively; S2, microinjecting active gRNA1, gRNA2 and cas9 proteins into fertilized eggs of the mouse, transplanting the fertilized eggs survived after injection into the body of the false pregnant female mouse, and obtaining an F0-generation mouse after the false pregnant female mouse is pregnant and farrowing; S3, mating the F0-generation mouse obtained in the step S2 with a wild-type mouse to obtain an F1-generation heterozygote; and S4, carrying out inbreeding on the F1-generation heterozygote obtained in the step S3 to obtain F2-generation mice, namely the IL21 gene knockout mouse model. Based on the CRISPR / Cas9 technology, the mouse animal model is established by knocking out an exon3-exon5 region in an ENSMUST000029273.7 transcript of an IL21 gene for the first time by designing reasonable gene sequences of specific target sites gRNA1 and gRNA2, and the mouse animal model can be applied to related research after tumor healing.
Owner:成都药康生物科技有限公司

Establishment of C578L/6 mouse animal model of transbuman senile dementia APP gene and use

InactiveCN1686567AHave a behavioral disorderIn-vivo testing preparationsMicroorganismMale pronucleus
A mouse animal model C57BL / 6 for the APP gene of human presenile dementia (AD) is disclosed, which can be used to research the AD, develop its medicine and screen its vaccine. Its configuring process includes using PDGF promoter to regulate APP and configure its transgene, injecting it in the male pronucleus of fertilized ovum of mouse, PCR, southern discrimination to obtain transgenic male animal, cesarean section, and DNA recombination-inbred-line passage for configuring its line.
Owner:INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI

sgRNA targeting sequence specifically targeting mouse Krt14 gene and application thereof

The invention discloses a sgRNA targeting sequence specifically targeting a mouse Krt14 gene and application thereof, and belongs to the technical fields of medical genetics and molecular biology. A nucleotide sequence corresponding to sgRNA is any one of four sequences of SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention further discloses a method for editing the mouse Krt14 gene by using the sgRNA targeting sequencing specifically targeting the mouse Krt14 gene. The sgRNA targeting sequence can mediate the efficient cleavage of target DNA by Cas9 protein, and then be usedfor editing the mouse Krt14 gene to affect the function of protein encoded by the mouse Krt14 gene. The sgRNA targeting sequence can achieve efficient targeting by a CRISPR / Cas9 system, and the efficiency is 70%, 90%, 65% and 100% separately.
Owner:人科(北京)生物技术有限公司

Primers, probe, kit and method for RPA-LFD visualization rapid detection of Schistosoma nucleic acid

The invention discloses primers, probe, kit and method for the RPA-LFD visualization rapid detection of Schistosoma nucleic acid. Sequences of the primers are as shown in SEQ ID NO.1-2, and a sequenceof the probe is as shown in SEQ ID NO.3. The RPA primers and probe are designed with the SjCHGCS19 gene as a target sequence, and the sensitive and rapid visualization detection of the schistosome nucleic acid is realized by using a RPA amplification technology combined with lateral flow chromatography test strip method. The detection method of the invention has a detection limit of 1 fg for Schistosoma japonicum genome DNA, and is expected to be used in the general detection of Schistosoma mansoni and Schistosoma haematobium. The method can detect circulating nucleic acid of Schistosoma in the serum of mice at an early stage of infection, the operation is simple and fast, no special equipment is required, a reaction temperature is close to room temperature, results can be observed with the naked eye, and the realization of the early and sensitive and rapid detection of intermediate hosts of Schistosoma on site, and the timely and sensitive monitoring of environments with high schistosomiasis transmission risks are facilitated.
Owner:中国疾病预防控制中心寄生虫病预防控制所国家热带病研究中心 +1

Construction method and application of preeclampsia mouse animal model

The invention discloses a construction method and application of a preeclampsia mouse animal model. According to the construction method, the m6A modification level in a mouse is reduced, and a mutant mouse with the reduced RNA m6A modification level, namely the preeclampsia mouse animal model, is obtained; specifically, a Mett13<+ / >hybrid mutant mouse is constructed through an ES targeting technology, and a result shows that the sensitivity of the mutant mouse to a PE inducer L-NAME is increased, the mutant mouse presents the characteristics of PE, and a new thought and a new means are provided for etiological diagnosis, prevention and treatment of preeclampsia.
Owner:SOUTHERN MEDICAL UNIVERSITY

Method for constructing B6-Chr1<ZZ2>/Xiao mouse model through replacing No. 1 chromosome based on ZZ2 mus musculus source

The invention relates to a method for constructing a B6-Chr1<ZZ2> / Xiao mouse model through replacing the No.1 chromosome based on a ZZ2 mus musculus source. The method comprises the steps as follows: a ZZ2 mus musculus is hybridized with a mouse C57BL / 6J, so as to obtain a F1-generation mouse; secondly, the F1-generation mouse is backcrossed with the C57BL / 6J, and a F2-generation mouse is obtained; thirdly, a mouse of which the No. 1 chromosome is in a heterozygosis state is screened out through genotyping; fourthly, the F2-generation mouse is backcrossed with the C57BL / 6J; fifthly, the same method is adopted for backcross until a 10-generation is screened out, and a mouse group of which the No.1 chromosomes are in the heterozygosis state while the other chromosomes are all pure C57BL / 6J genotypes is formed; sixthly, the DNA of the genes of the backcross mouse is identified; finally, when the backcross mouse with the No.1 chromosome in the heterozygosis state of ZZ2 and B6 is subjected to the 10-generation backcross, homologous genes carrying the No.1 chromosome of the ZZ2 mus musculus is bred and introduced into an inbred strain, and the mouse model is obtained through brother-sister selfing and gene identification.
Owner:DONGHUA UNIV

Urine biomarker for Japanese schistosomiasis early diagnosis, screening method, and application

The invention discloses a urine biomarker for Japanese schistosomiasis early diagnosis. The urine biomarker is one or more of a xanthurenic acid, a naphthalenesulfonic acid, or enanthylcarnitine. Sensitivity and specificity of the marker are greater than 0.9, and an area under the curve (AUC) is greater than 0.9, indicating that the three indicators have better predictability and may be used for the Japanese schistosomiasis early diagnosis. The invention further discloses a metabolomics-based screening method for the urine biomarker for the Japanese schistosomiasis early diagnosis. By constructing a mouse Japanese schistosomiasis model, ultra performance liquid chromatography-tandem mass spectrometry is used to perform metabolomics analysis on mouse urine, and a characteristic differentialmetabolite, that is, the biomarker for the Japanese schistosomiasis early diagnosis, between a normal mouse and a Japanese schistosomiasis-infected mouse is found and analyzed. The screening method is non-invasive, convenient and fast, and can accurately reflect a metabolic spectrum difference between the Japanese schistosomiasis-infected mouse and the normal mouse and have high specificity.
Owner:SUN YAT SEN UNIV

Construction method of VDR gene conditionality knockout floxed mouse model

PendingCN110408653AAvoid fatal restrictionsControl time specificityCell receptors/surface-antigens/surface-determinantsVector-based foreign material introductionSusceptibility/Resistance GeneMolecular biology
The invention belongs to the technical field of biology, and relates to a construction method of a VDR gene conditionality knockout floxed mouse model. According to the method, a gene targeting technique and an ES cell recombination technique are utilized, an Frt-neo-Frt-loxP sequence and an loxP sequence are respectively put on two sides of a VDR gene exon 3 of a bacterium artificial chromosome,a VDR-floxed carrier is constructed, and ES cells are guided through electric shock; after positive ES cells screened out through drug-resistant genes are cloned, through sequencing determination withPCR and a product thereof, the positive ES cells are transplanted in the body of a surrogacy female mouse, and a chimera mouse containing ES cell sources is produced; a male chimera mouse and a female wild type mouse intercross to generate an F1-generation mouse, and through sequencing confirmation with the PCR and the product thereof, a VDR-floxed heterozygote mouse containing Neo genes is obtained; and then the VDR-floxed heterozygote mouse containing Neo genes and an Flp mouse hybridize so that a VDR-floxed heterozygote mouse without neo genes is obtained. Through experimental verification, the VDR-floxed mouse model constructed by the construction method can lead to knockout of a VDR gene exon 3 region, and lead to deletion of VDR expression.
Owner:AFFILIATED HUSN HOSPITAL OF FUDAN UNIV

Construction method and application of SDK2 gene mutation mice mice

The invention provides a construction method of an SDK2 gene mutation mice model. The mice model comprises the following steps of designing and constructing sgRNA capable of specifically identifying an SDK2 gene on the basis of a CRISPR / Cas9 system, injecting the sgRNA and a targeting vector constructed on the basis of the sgRNA into a mice fertilized egg; and after embryo transplantation, screening out F0-generation mice with SDK2 gene mutation from the output mice, and hybridizing the F0-generation mice with wild type mice to obtain an F1-generation mice model with SDK2 gene mutation. The mice model constructed by the method can be stably passaged, the action mechanism of the SDK2 gene in mice hereditary cataract can be conveniently researched in practical application, and under the condition that human patient research materials are not easy to obtain and are restricted by medical ethics, the SDK2 gene can be stably cloned. The mice model provided by the invention can become an important tool in the research of hereditary cataract, and a research model capable of realizing stable heredity is provided in the research of pathogenesis, treatment methods, drug screening, cataract surgery and the like.
Owner:BEIJING TONGREN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV

Pharmaceutical compositions for treating melancholia associated with functional dyspepsia and preparation

The present invention discloses a medicine compound for curing melancholia and associated functional dyspepsia made from the raw materials including ginseng, morinda root and largehead atractylode. The present invention also discloses a preparation method of the medicine compound. The experiment proves that the medicine compound provided by the present invention can obviously reduce the immobility time in tail suspension of tested small mice and the accumulative immobility time in forced swimming of tested large mice, and promote the gastrointestinal power of tested large mice, thus inferring that the medicine compound provided by the present invention has the efficacy of resisting experimental melancholia and curing dyspepsia.
Owner:张作光

Preparation method of transgenic mouse of specificity expression Cre recombinase of hematopoietic system

InactiveCN102860282AVector-based foreign material introductionAnimal husbandryHuman Growth Hormone GeneMale pronucleus
The invention relates to a preparation method of a transgenic mouse of specificity expression Cre recombinase of a hematopoietic system. The invention discloses a transgenic mouse animal model, a deoxyribonucleic acid (DNA) fragment pEDAG-Cre is integrated in a genome of the transgenic mouse animal model, the DNA fragment comprises a promoter at an end of a gene (EDAG) 5' which is related to human embryonic development, a Cre recombinase gene and a human growth hormone gene polyA fragment. The invention also comprises a preparation method of the transgenic mouse animal model. The method comprises the steps of cloning 2.3 kb of a control region of the promoter at the end of the EDAG gene 5' by using a polymerase chain reaction (PCR) method, constructing a transgenic expression vector pEDAG-Cre, leading 5.6 kb of transgenic fragments into a male pronucleus of the mouse by using a micro-injection method, and identifying a positive transgenic mouse by using the PCR method. The RT-PCR method and a mouse-identifying result report ROSA26LacZ show that the specificity expression Cre recombinase of the transgenic mouse can be achieved only in the hematopoietic system.
Owner:INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA

Mouse typhus salmonella UF110lux and application of mouse typhus salmonella UF110lux in living body imaging

InactiveCN102757910AStable bioluminescence performanceStable light quantityBacteriaMicroorganism based processesMicrobiological cultureSalmonella
The invention relates to mouse typhus salmonella UF110lux and application of mouse typhus salmonella UF110lux in living body imaging. The strain is conserved in the China General Microbiological Culture Collection Centre specified by the State Intellectual Property Office on March 13, 2012, and the conservation number is CGMCCNo.5893. The mouse typhus salmonella UF110 is a strain obtained by the fact that a spv gene on a virulence plasmid is knockout, and can be used for researching the function of mouse typhus salmonella virulence gene spv as a control. The UF110lux is a strain which is constructed on the basis of the mouse typhus salmonella UF110 and has the stable biological luminescence property. According to the invention, the shortage of a traditional fluorescent mark is overcome, and the invasion, proliferation and diffusion of the mouse typhus salmonella in a living body of mice can be monitored dynamically in real time by using a living body imaging instrument. The invention provides a convenient tool for further study on a pathogenic mechanism of mouse typhus salmonella.
Owner:SUZHOU UNIV
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