71results about How to "Speed ​​up the R&D process" patented technology

The invention relates to a humanized gene genetically modified non-human animal, particularly a genetically modified rodent, especially a genetically modified mouse, and in particular relates to a construction method of a humanized PD-L1 gene animal model and application of the model in the biomedicine field.

The invention relates to a humanized gene genetically modified non-human animal, particularly a genetically modified rodent, especially a genetically modified mouse, and in particular relates to a construction method of a humanized CTLA-4 gene animal model and application of the model in the biomedicine field.

The invention relates to humanized gene modified non-human animals, especially genetically modified rodents, and especially genetically modified mice, and more specifically relates to a construction method of humanized CD137 gene animal models, and applications of the humanized CD137 gene animal models in biomedicine field.

The invention discloses an infrared-thermal-imaging-based continuous component bulk high-flux multi-field screening device and method, the device includes a control center, an experiment box, a loading platform, a temperature control module, a current control module, a vacuum generation device and an infrared thermal imaging system. In an experiment, different field excitations are inputted, according to different material characteristics, the upper surface of a to-be-detected sample has different temperature responses. Thermal radiation information of all sample surfaces is parallelly acquired. Color of each block of sample in a corresponding region in a thermal radiation energy image is identified by visual inspection to obtain temperature information. A temperature response curve of each block of sample is generated. According to a mathematical model of the test system, the physical characteristics of the material are calculated, and then screening results are obtained.

The invention discloses a material information cloud service system comprising a material data collection layer, a cloud service layer and an application layer. The material data collection layer comprises multiple sets of discretely distributed material data production equipment, wherein each set of material data production equipment is used for acquiring material data to be transmitted to the cloud service layer. The cloud service layer is used for receiving the material data and then analyzing the material data based on the preset analysis rules so as to form multiple material data analysis results. The application layer is used for acquiring the required material data and the material data analysis results of the required material data from the cloud service layer. The technical problems of high application limitation of the existing material data can be effectively solved so that the new material research and development process can be accelerated and the new material research and development time can be saved.

The invention provides a construction method of a CD226 gene humanized modified non-human animal. By utilizing a homologous recombination manner, a nucleotide sequence for encoding a human CD226 protein is introduced into a non-human animal genome. The humanized CD226 protein can be normally expressed in an animal body, and the non-human animal can be used as an animal model for researching humanCD226 signal mechanism and screening drugs for tumor and immune diseases, and has important application value for research and development of new drugs for immune targets. The invention also providesa CD226 chimeric protein, a CD226 chimeric gene, a targeting vector of the CD226 gene, the non-human animal constructed by the construction method, and an application of the non-human animal in the field of biological medicines.

The invention relates to a humanized genetically-modified non-human animal, in particular a genetically-modified rodent, but in particular to a genetically-modified mouse, and particularly relates toa building method of an animal model to express humanized CD3 protein and application of the animal model in the field of biomedicine.

The invention provides a novel wave generator cam design method. The method comprises the main steps of finite element model establishment, boundary condition introduction, analysis and calculation, fitting and the like. The outer edge curve of the cam designed by using the method conforms to a curve formula calculated by using the method instead of a standard ellipse, and the harmonic reducer ofthe wave generator cam designed by using the method can effectively reduce the problems of tooth surface abrasion, wave generator downward pressing, jumping, abnormal sound and the like.

The invention discloses a method for in-vitro culture of HEK293 for transient expression of protein. The method comprises the following steps: S1, resuscitating cryopreserved HEK293 cells in an original culture medium, and incubating the cryopreserved HEK293 cells in an incubator; S2, inoculating the cells into an HEK293 serum-free culture medium for culturing; S3, carrying out passage operation;S4, carrying out passage operation again; S5, preparing transfection plasmids; S6, acquiring data including cell density and survival rate, and diluting the cells; S7, diluting plasmid DNA; S8, diluting a transfection reagent; S9, adding the diluted transfection reagent into the diluted plasmid DNA, and incubating the mixture at room temperature; S10, slowly adding the mixture into the cells in the step S6, and performing shaking; and S11, carrying out culturing in an incubator, and harvesting the HEK293 cells and expression products. According to the invention, the expression quantity can beobviously increased, the expression efficiency is high, and more protein can be obtained in a shorter time.

The invention discloses a high-speed load dynamic pressure bearing testing system. The testing system comprises a testing table, a cooling system, a lubricating system, and an electric control system, wherein the testing table comprises a motor, a dynamic pressure bearing, a flywheel, a shaft coupler and a testing base; the cooling system and the lubricating system are connected through a pipeline testing table and are used for respectively providing the cooling function and the lubricating function for the testing system; the electric control system is connected with the testing table through a cable; the remote control can be realized through a programmable controller and a frequency converter, the starting speed, the stopping speed and the rotating speed of the motor can be changed; various possible working conditions in actual use are systematically simulated and analyzed, and the system can be monitored in real time through a temperature sensor and a vibration sensor; and therefore, the reliability of product research and development is guaranteed, the research and development time is shortened, and the research and development progress is accelerated.

The invention relates to the technical field of antibodies, in particular to a novel coronavirus Delta mutant strain specific antibody and application thereof. The invention provides an antibody specifically combined with a novel coronavirus Delta mutant strain, and the antibody can be used as a specific antibody for Delta mutant strain antigen detection, is used for identifying a novel coronavirus vaccine designed for the Delta mutant strain, and is used for quantitatively detecting the Spike protein content expressed by the vaccine. The antibody can be used for quality control of novel coronavirus vaccines and immunogenicity detection of clinical and preclinical research, or can be used as a quality control antibody for detection of protective antibodies in serum after vaccination.

The invention discloses a joint torque determination method for a four-footed wall-climbing robot with different postures. The method comprises the steps that adsorption posture information of the robot is described by adopting an adsorption posture; the robot adsorption posture and the leg tail end position are determined; the gravity of the robot is processed in a centralized mode based on the force translation principle and a centroid formula, and an overall force spiral balance equation of the robot under any adsorption posture is established; a static flexibility model of the leg supporting chain sub-assembly is established by utilizing finite element analysis to obtain a leg static flexibility model under any adsorption posture and linear deformation of the leg tail end through linear transformation, then a constraint equation is obtained and foot end force is solved; after the foot end force is obtained, joint torques of the robots with different postures can be obtained through mechanical analysis. According to the method for determining the joint torque of the four-foot wall-climbing robot with different postures, the foot end force of the four feet of the robot can be rapidly obtained, the torque of each joint of the robot can be determined, the method can be used for joint motor model selection and checking, and the research and development process of the robot is further accelerated.

The invention discloses a near-field communication modulation depth adaptive method and device and relates to the field of communication. The device comprises a master control chip, a non-contact chip and a first storage zone. The master control chip can control the non-contact chip to output the modulation-free amplitude according to a value of a first register; a numerical value is acquired from a data table or through calculation and written in a second register according to the data table stored in the first storage zone in advance and the measured value of the modulation-free amplitude, the non-contact chip is controlled to output the modulated amplitude according to the value of the second register, and the modulation depth is adaptively adjusted through control over the modulated amplitude. By the adoption of the technical scheme, the modulation depth can be kept within a reasonable range and the stability of data transmission in non-contact communication is improved.

The invention provides a siRNA molecule capable of inhibiting the expression of the COL1A1 gene in human beings and animals and a pharmaceutical composition, and application thereof. The siRNA molecule provided by the invention has long-acting effect in inhibiting the expression of the COL1A1 gene in human beings and animals, and can treat or improve hepatic fibrosis and hepatic diseases related to hepatic fibrosis.

The invention provides a construction method of a humanized CD40 gene modified animal model and an application thereof, and relates to the field of gene engineering. The CD40 gene humanized model animal is successfully prepared, the humanized CD40 protein can be normally expressed in the model body, and the product can be used for CD40 gene function research and humanized CD40 antibody screening and evaluation. The animal model prepared by the method can be applied to drug screening, drug effect research, immune related diseases, tumor treatment and the like for human CD40 target sites, the research and development process of new drugs is accelerated, the time and cost are saved, and the drug development risk is reduced. A powerful tool is provided for researching the function of the CD40 protein and screening tumor drugs.

The invention provides a main drive bearing test bench and a test system. The test bench comprises a bench frame; a power mechanism is integrated on the bench frame, a test bearing system is installedin the bench frame, and the test bearing system is connected with the power mechanism. According to the invention, through synchronous operation of the two sets of bearing systems, elimination of friction revolute pairs, loading of the test bearing system and transmission of the bearing force of the test bearing system to the to-be-tested bearing system, the performance test of the to-be-tested bearing system is further realized, and the stress condition of the main drive bearing under the real working condition is simulated; therefore, the test result is greatly improved to be close to the real environment, the powerful data support is conveniently provided for subsequent product development, the overall structural design is compact, equipment is highly integrated, and the operation reliability is improved.

The invention discloses a preparation method of a miniature pig with a human complement regulatory protein knocked into a Rosa26 site at a fixed point, which is characterized in that a human complement regulatory protein gene is inserted into a pig ROSA26 gene site in a fixed-point gene recombination mode, so that the miniature pig with constitutive expression of the human complement regulatory protein is constructed. The constructed miniature pig not only avoids the unclear genetic background caused by exogenous random insertion and the unstable expression quantity caused by chromosome separation in the progeny breeding process, but also provides important animal resources for the research of xenotransplantation and immune related diseases in the future, and has important clinical application value.

The invention provides a method for automatically detecting system I2C address abnormity based on sequence traversal. The method comprises the steps of designing a detection process of current BMC I2Cbus address abnormality based on layer sequence traversal, automatically scanning all slave devices under a BMC I2C bus layer by layer according to the detection process, determining actual addressesof the slave devices under the current BMC I2C bus, obtaining a preset address table, comparing the actual address of the slave device under the current BMC I2C bus with the preset address table, determining whether the current I2C address is abnormal or not,if the I2C address is not abnormal, marking the equipment as an effective level,and if the I2C address is abnormal, carrying out early warning and outputting an abnormal equipment address, and ending the detection process. The method has the beneficial effects that the method is used for effectively improving the detection efficiency of the address exception under the BMC I2C bus, saving manpower and material resources and accelerating the project research and development process, and is also suitable for the situation that other devices serve as hosts and downlink hitching is conducted on numerous I2C devices.

The invention relates to a financial asset transaction cloud system based on a block chain. The financial asset transaction cloud system comprises an IaaS layer, a PaaS layer, an SaaS layer and a client Client layer. The SaaS layer is a financial asset transaction cloud platform based on a block chain, and the transaction cloud platform comprises a user side asset transaction platform and a business side operation management platform; the asset transaction platform comprises a client center, an account center, a product and service center, a data center and an operation marketing center. The operation management platform comprises an operation management system, a risk monitoring management system and an operation and maintenance monitoring management system. The asset transaction platformforms the block chain composed of a financial transaction supervision mechanism, an asset registration and circulation mechanism, a personal transaction mechanism and an enterprise transaction mechanism. The PaaS layer comprises a service registration center, a data service layer, a basic service layer, a service warehouse layer, an operation and maintenance management platform, a risk managementplatform and an IDE development platform.

The invention relates to the field of drug-target interaction prediction technology and deep learning, in particular to a drug-target interaction prediction algorithm based on graph convolution and similarity. Comprising the following steps: 1) acquiring related data of drugs, proteins and DTI through public databases such as DygBank and RCSB; 2) extracting intermolecular structure information through global structure similarity; 3) extracting monomer characteristic information of the drug molecules and the protein molecules through DeepChem; 4) obtaining a drug-target interaction prediction result through feature dimension reduction, feature fusion and normalization processing; and 5) parameter adjustment and optimization are performed on the model, the prediction accuracy is continuously improved, and the optimal model is used for assisting research on drug discovery, drug verification, drug side effects and the like.

The invention discloses a physical simulation and calibration device and method for formation pressure testing. The physical simulation and calibration device comprises a rock core clamper and furthercomprises a confining pressure simulation module, a formation pressure simulation module, an annulus pressure simulation module, a suction system, a pushing force simulation module and a driving control system; the rock core clamper is internally provided with a rock core, the pushing force simulation module is provided with a thrust rod, and the thrust rod penetrates through the cavity wall of one side of the rock core clamper; the front end of the thrust rod is provided with a simulation probe, and the suction system is connected to the thrust rod; and the confining pressure simulation module, the formation pressure simulation module, the annulus pressure simulation module, the pushing force simulation module and the suction system are all connected with the driving control system. Thephysical simulation and calibration device has the beneficial effects that the physical simulation and calibration device can simulate the physical environment of the formation pressure testing, physical simulation of formation pressure testing is achieved, a formation pressure tester can be corrected and calibrated, and the formation pressure testing ability and control precision are improved.

The invention provides a construction method of an IL-13 gene humanized non-human animal of the IL-13 gene humanized non-human animal and an application in the field of biological medicine. The invention further provides a targeting vector, a humanized IL-13 gene, an IL-13 gene humanized cell and an application of the IL-13 gene humanized cell. According to the non-human animal obtained through the construction method, the human IL-13 protein specifically combined with the human IL-13 antibody can be normally expressed in vivo, and the non-human animal can be further used for preparing a disease animal model and evaluating and screening the in-vivo drug effect of the human IL-13 antibody.

The invention relates to a software-and-hardware integrated satellite navigation signal processing method. The method comprises the following steps of: (1), receiving data from two sources including asource containing intermediate frequency data collected by a receiving antenna and a radio frequency front end and a source containing data inputted by a data input module; (2), inputting the collected intermediate frequency data into a hardware correlator or a software correlator for related processing; (3), designing a single-channel correlator for each satellite signal, driving the hardware correlator by a clock signal, driving the software correlator by a logic clock signal, and enabling the mathematical models of the hardware correlator and the software correlator to be consistent and tohave the same functions; and (4), inputting a result of the correlator into a subsequent baseband processing and navigation resolving module, and adopting an HAL design for a baseband processing andnavigation resolving program to enable a receiving algorithm designed based on a software receiver to be directly transplanted to a hardware receiver module. According to the invention, the foundationis provided for direct application of a receiving algorithm result researched and developed based on a software receiver to a hardware receiver.

The invention relates to a method and device for predicting charging and discharging performance of a battery cell, a storage medium and electronic equipment. The method comprises the steps: in the process of predicting the target performance of a to-be-produced target battery cell, for multiple different preset environment temperatures, obtaining the voltage actual measurement data and battery cell temperatures, measured at multiple moments at the preset environment temperatures, of the small soft package battery cell, wherein the target performance comprises the charging performance or the discharging performance; calibrating model parameters of a preset small soft package battery cell model corresponding to the preset environment temperature according to the voltage measured data and the battery cell temperature to obtain a target small soft package model after parameter calibration; obtaining battery cell design parameters corresponding to the target battery cell; establishing a target battery cell model corresponding to the target battery cell according to the battery cell design parameters and the target small soft package model; and predicting charging and discharging performance parameters of the target cell during constant-current rate charging or discharging through the target cell model.

The invention relates to a method and a device for determining the charge and discharge performance of a battery cell, a storage medium and electronic equipment, a preset target battery cell model can be obtained, wherein the target battery cell model is used for simulating the charge and discharge performance of a target battery cell at a preset environment temperature. The target battery cell comprises a battery cell made of at least two layers of coated electrodes; the process of constant-current rate charging or constant-current rate discharging of the target battery cell at the preset environment temperature is simulated according to a first preset current through the target battery cell model; target performance parameters of a target battery cell are determined according to the simulation result, and the target performance parameters comprise charging performance parameters when the target battery cell is subjected to constant-current rate charging at the preset environment temperature or discharging performance parameters when the target battery cell is subjected to constant-current rate discharging at the preset environment temperature.

The invention relates to a wire holder for a low-voltage fixed-type circuit breaker, and the wire holder comprises a plurality of independent wiring terminals, a cover plate, and positioning parts at two ends. A guide rail passes through the positioning parts, the guide rail installation holes on all substrates and the cover plate to be connected with the positioning part. Moreover, each wiring terminal comprises a substrate, a long contact piece, a short contact piece, a wire pressing plate, a bolt, and a nut. Each substrate comprises a partitioning plate and a seat body, which are integrated together. The vertical side of each seat body is provided with short and long contact piece grooves from the front to the back, and each seat body is provided with a guide installation hole for allowing the guide rail to pass. The long contact pieces are disposed in the long contact piece grooves of the seat bodies, and the bolts pass through the via holes of the wire pressing plates and the long contact pieces to be connected with the screws in external limiting grooves. The short contact pieces are disposed in the short contact piece grooves of the seat bodies, and the bolts pass through the via holes of the wire pressing plates and the short contact pieces to be connected with the nuts in internal limiting grooves. The wire holder is simple in structure, is convenient to install, can achieve miniaturization, is suitable for different current levels, and can meet the vertical wiring demands of a user.

The invention discloses a method for improving precision of positioning defects on chip to a graphic layer. The method comprises the steps of: adding minimal repeated complete unit information to a silicon wafer according to a defect result observed by electron beam scanning; and reassigning coordinates to each defect according to the setting of an original point of an original complete unit of the minimal repeated complete unit, so as to improve the positioning of subsequent silicon wafer with the graphic layer. The method can automatically add the minimal repeated complete unit information,and improve the precision of positioning the defects in the bare wafer to later wafers with graphic layers.

The invention belongs to the field of a metal material, and particularly relates to a method for preparing oxide dispersion strengthened steel through high-flux continuous smelting. The method particularly comprises the steps of S1, selecting all raw materials according to components of the oxide dispersion strengthened steel of different oxide particles or clusters needed to be prepared by design: oxides of iron and mother alloy containing rare earth; S2, placing the oxides of the iron with different proportions into multiple smelting containers for standby application; and S3, heating and smelting the mother alloy containing rare earth, dropping in the smelting containers with the oxides of iron, and after cooling, obtaining multiple kinds of oxide dispersion strengthened steel with different contents. According to the method, the advantages of a method of smelting preparing of ODS are fused, ODS steel of different alloy components can be rapidly prepared largely within the extremelyshort time, meanwhile, the ODS steel prepared through the method can meet the subsequent heat machining, heat treatment and property detection no matter size or quality, and the development time andcost of research preparing can be greatly reduced.