71results about How to "Speed ​​up the R&D process" patented technology

The invention relates to a humanized gene genetically modified non-human animal, particularly a genetically modified rodent, especially a genetically modified mouse, and in particular relates to a construction method of a humanized PD-L1 gene animal model and application of the model in the biomedicine field.

The invention discloses an infrared-thermal-imaging-based continuous component bulk high-flux multi-field screening device and method, the device includes a control center, an experiment box, a loading platform, a temperature control module, a current control module, a vacuum generation device and an infrared thermal imaging system. In an experiment, different field excitations are inputted, according to different material characteristics, the upper surface of a to-be-detected sample has different temperature responses. Thermal radiation information of all sample surfaces is parallelly acquired. Color of each block of sample in a corresponding region in a thermal radiation energy image is identified by visual inspection to obtain temperature information. A temperature response curve of each block of sample is generated. According to a mathematical model of the test system, the physical characteristics of the material are calculated, and then screening results are obtained.

The invention discloses a material information cloud service system comprising a material data collection layer, a cloud service layer and an application layer. The material data collection layer comprises multiple sets of discretely distributed material data production equipment, wherein each set of material data production equipment is used for acquiring material data to be transmitted to the cloud service layer. The cloud service layer is used for receiving the material data and then analyzing the material data based on the preset analysis rules so as to form multiple material data analysis results. The application layer is used for acquiring the required material data and the material data analysis results of the required material data from the cloud service layer. The technical problems of high application limitation of the existing material data can be effectively solved so that the new material research and development process can be accelerated and the new material research and development time can be saved.

The invention relates to a humanized genetically-modified non-human animal, in particular a genetically-modified rodent, but in particular to a genetically-modified mouse, and particularly relates toa building method of an animal model to express humanized CD3 protein and application of the animal model in the field of biomedicine.

The invention provides a novel wave generator cam design method. The method comprises the main steps of finite element model establishment, boundary condition introduction, analysis and calculation, fitting and the like. The outer edge curve of the cam designed by using the method conforms to a curve formula calculated by using the method instead of a standard ellipse, and the harmonic reducer ofthe wave generator cam designed by using the method can effectively reduce the problems of tooth surface abrasion, wave generator downward pressing, jumping, abnormal sound and the like.

The invention discloses a method for in-vitro culture of HEK293 for transient expression of protein. The method comprises the following steps: S1, resuscitating cryopreserved HEK293 cells in an original culture medium, and incubating the cryopreserved HEK293 cells in an incubator; S2, inoculating the cells into an HEK293 serum-free culture medium for culturing; S3, carrying out passage operation;S4, carrying out passage operation again; S5, preparing transfection plasmids; S6, acquiring data including cell density and survival rate, and diluting the cells; S7, diluting plasmid DNA; S8, diluting a transfection reagent; S9, adding the diluted transfection reagent into the diluted plasmid DNA, and incubating the mixture at room temperature; S10, slowly adding the mixture into the cells in the step S6, and performing shaking; and S11, carrying out culturing in an incubator, and harvesting the HEK293 cells and expression products. According to the invention, the expression quantity can beobviously increased, the expression efficiency is high, and more protein can be obtained in a shorter time.

The invention discloses a high-speed load dynamic pressure bearing testing system. The testing system comprises a testing table, a cooling system, a lubricating system, and an electric control system, wherein the testing table comprises a motor, a dynamic pressure bearing, a flywheel, a shaft coupler and a testing base; the cooling system and the lubricating system are connected through a pipeline testing table and are used for respectively providing the cooling function and the lubricating function for the testing system; the electric control system is connected with the testing table through a cable; the remote control can be realized through a programmable controller and a frequency converter, the starting speed, the stopping speed and the rotating speed of the motor can be changed; various possible working conditions in actual use are systematically simulated and analyzed, and the system can be monitored in real time through a temperature sensor and a vibration sensor; and therefore, the reliability of product research and development is guaranteed, the research and development time is shortened, and the research and development progress is accelerated.

The invention relates to the technical field of antibodies, in particular to a novel coronavirus Delta mutant strain specific antibody and application thereof. The invention provides an antibody specifically combined with a novel coronavirus Delta mutant strain, and the antibody can be used as a specific antibody for Delta mutant strain antigen detection, is used for identifying a novel coronavirus vaccine designed for the Delta mutant strain, and is used for quantitatively detecting the Spike protein content expressed by the vaccine. The antibody can be used for quality control of novel coronavirus vaccines and immunogenicity detection of clinical and preclinical research, or can be used as a quality control antibody for detection of protective antibodies in serum after vaccination.

The invention provides a siRNA molecule capable of inhibiting the expression of the COL1A1 gene in human beings and animals and a pharmaceutical composition, and application thereof. The siRNA molecule provided by the invention has long-acting effect in inhibiting the expression of the COL1A1 gene in human beings and animals, and can treat or improve hepatic fibrosis and hepatic diseases related to hepatic fibrosis.

The invention provides a construction method of a humanized CD40 gene modified animal model and an application thereof, and relates to the field of gene engineering. The CD40 gene humanized model animal is successfully prepared, the humanized CD40 protein can be normally expressed in the model body, and the product can be used for CD40 gene function research and humanized CD40 antibody screening and evaluation. The animal model prepared by the method can be applied to drug screening, drug effect research, immune related diseases, tumor treatment and the like for human CD40 target sites, the research and development process of new drugs is accelerated, the time and cost are saved, and the drug development risk is reduced. A powerful tool is provided for researching the function of the CD40 protein and screening tumor drugs.

The invention discloses a preparation method of a miniature pig with a human complement regulatory protein knocked into a Rosa26 site at a fixed point, which is characterized in that a human complement regulatory protein gene is inserted into a pig ROSA26 gene site in a fixed-point gene recombination mode, so that the miniature pig with constitutive expression of the human complement regulatory protein is constructed. The constructed miniature pig not only avoids the unclear genetic background caused by exogenous random insertion and the unstable expression quantity caused by chromosome separation in the progeny breeding process, but also provides important animal resources for the research of xenotransplantation and immune related diseases in the future, and has important clinical application value.

The invention relates to the field of drug-target interaction prediction technology and deep learning, in particular to a drug-target interaction prediction algorithm based on graph convolution and similarity. Comprising the following steps: 1) acquiring related data of drugs, proteins and DTI through public databases such as DygBank and RCSB; 2) extracting intermolecular structure information through global structure similarity; 3) extracting monomer characteristic information of the drug molecules and the protein molecules through DeepChem; 4) obtaining a drug-target interaction prediction result through feature dimension reduction, feature fusion and normalization processing; and 5) parameter adjustment and optimization are performed on the model, the prediction accuracy is continuously improved, and the optimal model is used for assisting research on drug discovery, drug verification, drug side effects and the like.

The invention provides a construction method of an IL-13 gene humanized non-human animal of the IL-13 gene humanized non-human animal and an application in the field of biological medicine. The invention further provides a targeting vector, a humanized IL-13 gene, an IL-13 gene humanized cell and an application of the IL-13 gene humanized cell. According to the non-human animal obtained through the construction method, the human IL-13 protein specifically combined with the human IL-13 antibody can be normally expressed in vivo, and the non-human animal can be further used for preparing a disease animal model and evaluating and screening the in-vivo drug effect of the human IL-13 antibody.

The invention relates to a software-and-hardware integrated satellite navigation signal processing method. The method comprises the following steps of: (1), receiving data from two sources including asource containing intermediate frequency data collected by a receiving antenna and a radio frequency front end and a source containing data inputted by a data input module; (2), inputting the collected intermediate frequency data into a hardware correlator or a software correlator for related processing; (3), designing a single-channel correlator for each satellite signal, driving the hardware correlator by a clock signal, driving the software correlator by a logic clock signal, and enabling the mathematical models of the hardware correlator and the software correlator to be consistent and tohave the same functions; and (4), inputting a result of the correlator into a subsequent baseband processing and navigation resolving module, and adopting an HAL design for a baseband processing andnavigation resolving program to enable a receiving algorithm designed based on a software receiver to be directly transplanted to a hardware receiver module. According to the invention, the foundationis provided for direct application of a receiving algorithm result researched and developed based on a software receiver to a hardware receiver.

The invention relates to a method and device for predicting charging and discharging performance of a battery cell, a storage medium and electronic equipment. The method comprises the steps: in the process of predicting the target performance of a to-be-produced target battery cell, for multiple different preset environment temperatures, obtaining the voltage actual measurement data and battery cell temperatures, measured at multiple moments at the preset environment temperatures, of the small soft package battery cell, wherein the target performance comprises the charging performance or the discharging performance; calibrating model parameters of a preset small soft package battery cell model corresponding to the preset environment temperature according to the voltage measured data and the battery cell temperature to obtain a target small soft package model after parameter calibration; obtaining battery cell design parameters corresponding to the target battery cell; establishing a target battery cell model corresponding to the target battery cell according to the battery cell design parameters and the target small soft package model; and predicting charging and discharging performance parameters of the target cell during constant-current rate charging or discharging through the target cell model.

The invention relates to a wire holder for a low-voltage fixed-type circuit breaker, and the wire holder comprises a plurality of independent wiring terminals, a cover plate, and positioning parts at two ends. A guide rail passes through the positioning parts, the guide rail installation holes on all substrates and the cover plate to be connected with the positioning part. Moreover, each wiring terminal comprises a substrate, a long contact piece, a short contact piece, a wire pressing plate, a bolt, and a nut. Each substrate comprises a partitioning plate and a seat body, which are integrated together. The vertical side of each seat body is provided with short and long contact piece grooves from the front to the back, and each seat body is provided with a guide installation hole for allowing the guide rail to pass. The long contact pieces are disposed in the long contact piece grooves of the seat bodies, and the bolts pass through the via holes of the wire pressing plates and the long contact pieces to be connected with the screws in external limiting grooves. The short contact pieces are disposed in the short contact piece grooves of the seat bodies, and the bolts pass through the via holes of the wire pressing plates and the short contact pieces to be connected with the nuts in internal limiting grooves. The wire holder is simple in structure, is convenient to install, can achieve miniaturization, is suitable for different current levels, and can meet the vertical wiring demands of a user.

The invention belongs to the field of a metal material, and particularly relates to a method for preparing oxide dispersion strengthened steel through high-flux continuous smelting. The method particularly comprises the steps of S1, selecting all raw materials according to components of the oxide dispersion strengthened steel of different oxide particles or clusters needed to be prepared by design: oxides of iron and mother alloy containing rare earth; S2, placing the oxides of the iron with different proportions into multiple smelting containers for standby application; and S3, heating and smelting the mother alloy containing rare earth, dropping in the smelting containers with the oxides of iron, and after cooling, obtaining multiple kinds of oxide dispersion strengthened steel with different contents. According to the method, the advantages of a method of smelting preparing of ODS are fused, ODS steel of different alloy components can be rapidly prepared largely within the extremelyshort time, meanwhile, the ODS steel prepared through the method can meet the subsequent heat machining, heat treatment and property detection no matter size or quality, and the development time andcost of research preparing can be greatly reduced.