Method for constructing B6-Chr1<ZZ2>/Xiao mouse model through replacing No. 1 chromosome based on ZZ2 mus musculus source

Abstract

The invention relates to a method for constructing a B6-Chr1<ZZ2> / Xiao mouse model through replacing the No.1 chromosome based on a ZZ2 mus musculus source. The method comprises the steps as follows: a ZZ2 mus musculus is hybridized with a mouse C57BL / 6J, so as to obtain a F1-generation mouse; secondly, the F1-generation mouse is backcrossed with the C57BL / 6J, and a F2-generation mouse is obtained; thirdly, a mouse of which the No. 1 chromosome is in a heterozygosis state is screened out through genotyping; fourthly, the F2-generation mouse is backcrossed with the C57BL / 6J; fifthly, the same method is adopted for backcross until a 10-generation is screened out, and a mouse group of which the No.1 chromosomes are in the heterozygosis state while the other chromosomes are all pure C57BL / 6J genotypes is formed; sixthly, the DNA of the genes of the backcross mouse is identified; finally, when the backcross mouse with the No.1 chromosome in the heterozygosis state of ZZ2 and B6 is subjected to the 10-generation backcross, homologous genes carrying the No.1 chromosome of the ZZ2 mus musculus is bred and introduced into an inbred strain, and the mouse model is obtained through brother-sister selfing and gene identification.

Description

technical field [0001] The invention belongs to the field of animal model construction, in particular to a chromosome 1 replacement B6-Chr1 derived from ZZ2 Mus musculus ZZ2 / Xiao mouse model construction method. Background technique [0002] Mus musculus Mus musculus originated in South Asia and migrated to various parts of Asia and Europe about 500,000 to 1,000,000 years ago, forming three main subspecies of Mus musculus, M.m.musculus, M.m.castaneus and M.m.domesticus. Although all three subspecies of Mus musculus are ancestors of today's laboratory mice, the common classical inbred mouse genome is primarily derived from M.m. domesticus. There are at least two subspecies of Mus musculus, M.m.castaneus and M.m.musculus, in China. There are large differences between the genetic structure of Mus musculus populations in my country and the current experimental mouse strains, so it is a very important supplementary resource for related gene research. [0003] Although there ...

AI Technical Summary

Problems solved by technology

The fundamental reason is that the current experimental animal strains all come from a small number of primitive ancestors (pet mice), which brings a problem: for each gene, in the population (such as all hundreds of mouse experimental strains) considered as a group) the number of alleles is small, much lower than that of Mus musculus musculus subspecies in the natural state

[0004] Especially considering that the population itself belongs to a group with high genetic heterogeneity, it is often too simple to evaluate drug effects and other effects with simple inbred mouse models.

The single source of genetic background of existing inbred mice often makes a drug effective in mice but ineffective in large populations; or the opposite result

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