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Application of curcumol in intervening endometrial stromal cells

A technology of endometrium and curcumol, which is applied in the field of biomedicine to achieve the effect of inhibiting proliferation, inducing the generation of reactive oxygen species in cells, and promoting the generation of reactive oxygen species in apoptotic cells

Active Publication Date: 2017-01-25
江西省妇幼保健院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report that curcumol can treat adenomyosis and that it can treat adenomyosis by interfering with the proliferation, invasion, migration, and apoptosis of endometrial stromal cells.

Method used

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  • Application of curcumol in intervening endometrial stromal cells
  • Application of curcumol in intervening endometrial stromal cells
  • Application of curcumol in intervening endometrial stromal cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Isolation of primary endometrial stromal cells:

[0017] 1) Put the collected fresh endometrial tissue into an ultra-clean platform plate, and cut it to 1mm with sterilized scissors 3 , washed with PBS;

[0018] 2) Add 9mL DMEM / F-12+1mL diluted type IV collagenase, mix well and transfer to a centrifuge tube;

[0019] 3) Put it into a preheated (37°C) clean water bath, keep it in a constant temperature water bath for 120-180 minutes, shake and mix every 15 minutes;

[0020] 4) The tissue suspension in the ultra-clean bench passes through an 80-mesh sterilization screen;

[0021] 5) Pass the filtrate through a 300-mesh sieve, and absorb the filtered liquid to the centrifuge tube;

[0022] 6) Centrifuge at 1000rpm for 6-8min;

[0023] 7) Discard the supernatant, add PBS solution in the tube and blow evenly;

[0024] 8) Centrifuge at 1000rpm for 6-8min;

[0025] 9) Discard the supernatant, add 10% FBS culture medium in the centrifuge tube and resuspend to obtain t...

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Abstract

The invention discloses application of curcumol in intervening endometrial stromal cells; curcumol is a traditional Chinese medicinal monomer compound with a molecular formula of C15H24O2 and a molecular weight of 236.2. The compound can well inhibit the proliferation, invasion and migration of endometrial stromal cells and is also effective in promoting apoptosis and inducing generation of intracellular reactive oxygen.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an application of curcumol intervening endometrial stromal cells. Background technique [0002] Adenomyosis is a disease in which endometrial glands and stromal cells infiltrate and grow diffusely in the myometrium. It mainly occurs in women of childbearing age. refractory disease. Our and previous studies have found that endometrial stromal cells play an important role in the pathogenesis of adenomyosis and can be used as targets for the treatment of adenomyosis. [0003] Clinically, hormones or surgery are often used in the treatment of adenomyosis, but hormone therapy has a lot of side effects, and surgery has certain indications, and the recurrence rate is high, both of which have certain limitations. In recent years, traditional Chinese medicines have shown relatively reliable curative effects in the treatment of adenomyosis without obvious adverse reactions. [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/35A61P15/00
CPCA61K31/35
Inventor 邹阳刘发英杨必成张子宇罗勇
Owner 江西省妇幼保健院
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