Use of curcumol in the preparation of medicines for treating adenomyosis by interfering with endometrial stromal cells
A technology for adenomyosis and endometrium, which is applied in the direction of drug combinations, diseases, active ingredients of heterocyclic compounds, etc., to achieve the effects of inhibiting proliferation, inducing the generation of reactive oxygen species in cells, and promoting the generation of reactive oxygen species in apoptotic cells
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[0016] (1) Isolation of primary endometrial stromal cells:
[0017] 1) Put the collected fresh endometrial tissue into an ultra-clean platform plate, and cut it to 1mm with sterilized scissors 3 , washed with PBS;
[0018] 2) Add 9mL DMEM / F-12+1mL diluted type IV collagenase, mix well and transfer to a centrifuge tube;
[0019] 3) Put it into a preheated (37°C) clean water bath, keep it in a constant temperature water bath for 120-180 minutes, shake and mix every 15 minutes;
[0020] 4) The tissue suspension in the ultra-clean bench passes through an 80-mesh sterilization screen;
[0021] 5) Pass the filtrate through a 300-mesh sieve, and absorb the filtered liquid to the centrifuge tube;
[0022] 6) Centrifuge at 1000rpm for 6-8min;
[0023] 7) Discard the supernatant, add PBS solution in the tube and blow evenly;
[0024] 8) Centrifuge at 1000rpm for 6-8min;
[0025] 9) Discard the supernatant, add 10% FBS culture medium in the centrifuge tube and resuspend to obtain t...
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